| 基因名称: | APAF1 |
|---|---|
| 基因又名: | APAF-1; CED4 |
| Gene ID: | 317 |
| 种属 | Homo sapiens |
| DNA序列编号: | NM_013229.3 |
| DNA描述 | Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 1, mRNA |
| DNA大小: | 7168 bp |
| DNA编码区: |
atggatgcaaaagctcgaaattgtttgcttcaacatagagaagctctggaaaaggacatc
aagacatcctacatcatggatcacatgattagtgatggatttttaacaatatcagaagag
gaaaaagtaagaaatgagcccactcaacagcaaagagcagctatgctgattaaaatgata
cttaaaaaagataatgattcctacgtatcattctacaatgctctactacatgaaggatat
aaagatcttgctgcccttctccatgatggcattcctgttgtctcttcttccagtgtaagg
acagtcctgtgtgaaggtggagtaccacagaggccagttgtttttgtcacaaggaagaag
ctggtgaatgcaattcagcagaagctctccaaattgaaaggtgaaccaggatgggtcacc
atacatggaatggcaggctgtgggaagtctgtattagctgcagaagctgttagagatcat
tcccttttagaaggttgtttcccagggggagtgcattgggtttcagttgggaaacaagac
aaatctgggcttctgatgaaactgcagaatctttgcacacggttggatcaggatgagagt
ttttcccagaggcttccacttaatattgaagaggctaaagaccgtctccgcattctgatg
cttcgcaaacacccaaggtctctcttgatcttggatgatgtttgggactcttgggtgttg
aaagcttttgacagtcagtgtcagattcttcttacaaccagagacaagagtgttacagat
tcagtaatgggtcctaaatatgtagtccctgtggagagttccttaggaaaggaaaaagga
cttgaaattttatccctttttgttaatatgaagaaggcagatttgccagaacaagctcat
agtattataaaagaatgtaaaggctctccccttgtagtatctttaattggtgcactttta
cgtgattttcccaatcgctgggagtactacctcaaacagcttcagaataagcagtttaag
agaataaggaaatcttcgtcttatgattatgaggctctagatgaagccatgtctataagt
gttgaaatgctcagagaagacatcaaagattattacacagatctttccatccttcagaag
gacgttaaggtgcctacaaaggtgttatgtattctctgggacatggaaactgaagaagtt
gaagacatactgcaggagtttgtaaataagtctcttttattctgtgatcggaatggaaag
tcgtttcgttattatttacatgatcttcaagtagattttcttacagagaagaattgcagc
cagcttcaggatctacataagaagataatcactcagtttcagagatatcaccagccgcat
actctttcaccagatcaggaagactgtatgtattggtacaactttctggcctatcacatg
gccagtgccaagatgcacaaggaactttgtgctttaatgttttccctggattggattaaa
gcaaaaacagaacttgtaggccctgctcatctgattcatgaatttgtggaatacagacat
atactagatgaaaaggattgtgcagtcagtgagaattttcaggagtttttatctttaaat
ggacaccttcttggacgacagccatttcctaatattgtacaactgggtctctgtgagccg
gaaacttcagaagtttatcagcaagctaagctgcaggccaagcaggaggtcgataatgga
atgctttacctggaatggataaacaaaaaaaacatcacgaatctttcccgcttagttgtc
cgcccccacacagatgctgtttaccatgcctgcttttctgaggatggtcagagaatagct
tcttgtggagctgataaaaccttacaggtgttcaaagctgaaacaggagagaaacttcta
gaaatcaaggctcatgaggatgaagtgctttgttgtgcattctctacagatgacagattt
atagcaacctgctcagtggataaaaaagtgaagatttggaattctatgactggggaacta
gtacacacctatgatgagcactcagagcaagtcaattgctgccatttcaccaacagtagt
catcatcttctcttagccactgggtcaagtgactgcttcctcaaactttgggatttgaat
caaaaagaatgtcgaaataccatgtttggtcatacaaattcagtcaatcactgcagattt
tcaccagatgataagcttttggctagttgttcagctgatggaaccttaaagctttgggat
gcgacatcagcaaatgagaggaaaagcattaatgtgaaacagttcttcctaaatttggag
gaccctcaagaggatatggaagtgatagtgaagtgttgttcgtggtctgctgatggtgca
aggataatggtggcagcaaaaaataaaatctttctttttgacattcatactagtggccta
ttgggagaaatccacacgggccatcacagcaccatccagtactgtgacttctccccacaa
aaccatttggcagtggttgctttgtcccagtactgtgtagagttgtggaatacagactca
cgttcaaaggtggctgattgcagaggacatttaagttgggttcatggtgtgatgttttct
cctgatggatcatcatttttgacatcttctgatgaccagacaatcaggctctgggagaca
aagaaagtatgtaagaactctgctgtaatgttaaagcaagaagtagatgttgtgtttcaa
gaaaatgaagtgatggtccttgcagttgaccatataagacgtctgcaactcattaatgga
agaacaggtcagattgattatctgactgaagctcaagttagctgctgttgcttaagtcca
catcttcagtacattgcatttggagatgaaaatggagccattgagattttagaacttgta
aacaatagaatcttccagtccaggtttcagcacaagaaaactgtatggcacatccagttc
acagccgatgagaagactcttatttcaagttctgatgatgctgaaattcaggtatggaat
tggcaattggacaaatgtatctttctacgaggccatcaggaaacagtgaaagactttaga
ctcttgaaaaattcaagactgctttcttggtcatttgatggaacagtgaaggtatggaat
attattactggaaataaagaaaaagactttgtctgtcaccagggtacagtactttcttgt
gacatttctcacgatgctaccaagttttcatctacctctgctgacaagactgcaaagatc
tggagttttgatctccttttgccacttcatgaattgaggggccacaacggctgtgtgcgc
tgctctgccttctctgtggacagtaccctgctggcaacgggagatgacaatggagaaatc
aggatatggaatgtctcaaacggtgagcttcttcatttgtgtgctccgctttcagaagaa
ggagctgctacccatggaggctgggtgactgacctttgcttttctccagatggcaaaatg
cttatctctgctggaggatatattaagtggtggaacgttgtcactggggaatcctcacag
accttctacacaaatggaaccaatcttaagaaaatacacgtgtcccctgacttcaaaaca
tatgtgactgtggataatcttggtattttatatattttacagactttagaataa
|
| 蛋白序列编号: | NP_037361.1 |
| 蛋白描述: | apoptotic protease-activating factor 1 isoform a [Homo sapiens] |
| 蛋白大小: | 1237 aa |
| 蛋白序列: |
MDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLTISEEEKVRNEPTQQQRAAMLIKMI
LKKDNDSYVSFYNALLHEGYKDLAALLHDGIPVVSSSSVRTVLCEGGVPQRPVVFVTRKK
LVNAIQQKLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPGGVHWVSVGKQD
KSGLLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWDSWVL
KAFDSQCQILLTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEILSLFVNMKKADLPEQAH
SIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEAMSIS
VEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDILQEFVNKSLLFCDRNGK
SFRYYLHDLQVDFLTEKNCSQLQDLHKKIITQFQRYHQPHTLSPDQEDCMYWYNFLAYHM
ASAKMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDCAVSENFQEFLSLN
GHLLGRQPFPNIVQLGLCEPETSEVYQQAKLQAKQEVDNGMLYLEWINKKNITNLSRLVV
RPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSTDDRF
IATCSVDKKVKIWNSMTGELVHTYDEHSEQVNCCHFTNSSHHLLLATGSSDCFLKLWDLN
QKECRNTMFGHTNSVNHCRFSPDDKLLASCSADGTLKLWDATSANERKSINVKQFFLNLE
DPQEDMEVIVKCCSWSADGARIMVAAKNKIFLFDIHTSGLLGEIHTGHHSTIQYCDFSPQ
NHLAVVALSQYCVELWNTDSRSKVADCRGHLSWVHGVMFSPDGSSFLTSSDDQTIRLWET
KKVCKNSAVMLKQEVDVVFQENEVMVLAVDHIRRLQLINGRTGQIDYLTEAQVSCCCLSP
HLQYIAFGDENGAIEILELVNNRIFQSRFQHKKTVWHIQFTADEKTLISSSDDAEIQVWN
WQLDKCIFLRGHQETVKDFRLLKNSRLLSWSFDGTVKVWNIITGNKEKDFVCHQGTVLSC
DISHDATKFSSTSADKTAKIWSFDLLLPLHELRGHNGCVRCSAFSVDSTLLATGDDNGEI
RIWNVSNGELLHLCAPLSEEGAATHGGWVTDLCFSPDGKMLISAGGYIKWWNVVTGESSQ
TFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILQTLE
|
cDNA克隆和表达质粒购买
| 货号 | 载体 | 片段 | 标签 | 表达系统 | 价格 |
|---|---|---|---|---|---|
| NM013229-1 | pOTB7: | 全长 | 无 | cDNA克隆 | 1000 |
| NM013229-2 | pET-28a | 全长 | N-His | 大肠 | 2800 |
| NM013229-3 | pET-21b | 全长 | C-His | 大肠 | 2800 |
| NM013229-4 | pET-32a | 全长 | N-His、N-Trx | 大肠 | 2800 |
| NM013229-5 | pGEX-4T-1 | 全长 | N-GST | 大肠 | 2800 |
| NM013229-6 | pcDNA3.1(+) | 全长 | 无 | 哺乳细胞 | 2800 |
| NM013229-7 | pcDNA3.1/His A | 全长 | N-His | 哺乳细胞 | 2800 |
| NM013229-8 | pcDNA3.1/MycHisA | 全长 | C-His、C-Myc | 哺乳细胞 | 2800 |
| NM013229-9 | pCMV-Tag 2A | 全长 | N-Flag | 哺乳细胞 | 2800 |
| NM013229-10 | pCMV-Tag 4A | 全长 | C-Flag | 哺乳细胞 | 2800 |
| NM013229-11 | pCMV-HA | 全长 | N-HA | 哺乳细胞 | 2800 |
| NM013229-12 | pEGFP-C1 | 全长 | N-EGFP | 哺乳细胞 | 2800 |
| NM013229-13 | pEGFP-N1 | 全长 | C-EGFP | 哺乳细胞 | 2800 |
| NM013229-14 | p3xFlag-CMV-10 | 全长 | N-3xFlag | 哺乳细胞 | 2800 |
| NM013229-15 | p3xFlag-CMV-14 | 全长 | C-3xflag | 哺乳细胞 | 2800 |
| NM013229-16 | pLVX-Puro | 全长 | 无 | 慢病毒 | 2800 |
| NM013229-17 | pLVX-AcGFP1-N1 | 全长 | C-AcGFP | 慢病毒 | 2800 |
| NM013229-18 | pLVX-AcGFP1-C1 | 全长 | N-AcGFP | 慢病毒 | 2800 |
| NM013229-19 | pLVX-IRES-zsGreen | 全长 | 无 | 慢病毒 | 2800 |
| NM013229-20 | pFastbacI | 全长 | 无 | 昆虫细胞 | 2800 |
| NM013229-21 | pFastbac-HTB | 全长 | N-His | 昆虫细胞 | 2800 |
| NM013229-22 | pFastbac-CHis | 全长 | C-His | 昆虫细胞 | 2800 |
| NM013229-23 | pFastbac-GST | 全长 | N-GST | 昆虫细胞 | 2800 |
蛋白购买
| 基因名称 | 表达系统 | 片段 | 标签 | 产品规格 | 价格 |
|---|---|---|---|---|---|
| APAF1 | 大肠 | 全长 | N-His | 100 ug | 3500 |
| APAF1 | 哺乳 | 全长 | N-His | 100 ug | 5500 |
| APAF1 | 昆虫 | 全长 | N-His | 100 ug | 5500 |
抗体购买
| 产品名称 | 品牌 | 价格 |
|---|---|---|
| APAF1抗体/ APAF1抗体 | abcam | 询价 |
| APAF1抗体/ APAF1抗体 | CST | 询价 |
| APAF1抗体/ APAF1抗体 | Santa Cruz | 询价 |
