基因名称: | DYSF |
---|---|
基因又名: | FER1L1; LGMD2B; LGMDR2; MMD1 |
Gene ID: | 8291 |
种属 | Homo sapiens |
DNA序列编号: | NM_001130987.2 |
DNA描述 | Homo sapiens dysferlin (DYSF), transcript variant 1, mRNA |
DNA大小: | 6775 bp |
DNA编码区: |
atgctgtgctgcctgctggtgagggccagcaacctccccagtgcgaagaaggaccggcgc
agcgaccctgtcgcaagcctgactttccgaggggtgaagaagagaaccaaagtcatcaag
aacagcgtgaaccctgtatggaatgagggatttgaatgggacctcaagggcatccccctg
gaccagggctctgagcttcatgtggtggtcaaagaccatgagacgatggggaggaacagg
ttcctgggggaagccaaggtcccactccgagaggtcctcgccacccctagtctgtccgcc
agcttcaatgcccccctgctggacaccaagaagcagcccacaggggcctcgctggtcctg
caggtgtcctacacaccgctgcctggagctgtgcccctgttcccgccccctactcctctg
gagccctccccgactctgcctgacctggatgtagtggcaggcgggggacagagccgggcc
gagacttggtccctgctcagtgacagcaccatggacacgagatactctggaaagaagtgg
ccggcccccacggacacaggaggagaggaagacacagaggaccagggactcactggagat
gaggcggagccattcctggatcaaagcggaggcccgggggctcccaccaccccaaggaaa
ctaccttcacgtcctccgccccactaccccgggatcaaaagaaagcgaagtgcgcctaca
tctagaaagctgctgtcagacaaaccgcaggatttccagatcagggtccaggtgatcgag
gggcgccagctgccgggggtgaacatcaagcctgtggtcaaggttaccgctgcagggcag
accaagcggacgcggatccacaagggaaacagcccactcttcaatgagactcttttcttc
aacttgtttgactctcctggggagctgtttgatgagcccatctttatcacggtggtagac
tctcgttctctcaggacagatgctctcctcggggagttccggatggacgtgggcaccatt
tacagagagccccggcacgcctatctcaggaagtggctgctgctctcagaccctgatgac
ttctctgctggggccagaggctacctgaaaacaagcctttgtgtgctggggcctggggac
gaagcgcctctggagagaaaagacccctctgaagacaaggaggacattgaaagcaacctg
ctccggcccacaggcgtagccctgcgaggagcccacttctgcctgaaggtcttccgggcc
gaggacttgccgcagatggacgatgccgtgatggacaacgtgaaacagatctttggcttc
gagagtaacaagaagaacttggtggacccctttgtggaggtcagctttgcggggaaaatg
ctgtgcagcaagatcttggagaagacggccaaccctcagtggaaccagaacatcacactg
cctgccatgtttccctccatgtgcgaaaaaatgaggattcgtatcatagactgggaccgc
ctgactcacaatgacatcgtggctaccacctacctgagtatgtcgaaaatctctgcccct
ggaggagaaatagaagtggatgactacctgggcttcctccccacttttgggccctgctac
atcaacctctatggcagtcccagagagttcacaggcttcccagacccctacacagagctc
aacacaggcaagggggaaggtgtggcttatcgtggccggcttctgctctccctggagacc
aagctggtggagcacagtgaacagaaggtggaggaccttcctgcggatgacatcctccgg
gtggagaagtaccttaggaggcgcaagtactccctgtttgcggccttctactcagccacc
atgctgcaggatgtggatgatgccatccagtttgaggtcagcatcgggaactacgggaac
aagttcgacatgacctgcctgccgctggcctccaccactcagtacagccgtgcagtcttt
gacgggtgccactactactacctaccctggggtaacgtgaaacctgtggtggtgctgtca
tcctactgggaggacatcagccatagaatcgagactcagaaccagctgcttgggattgct
gaccggctggaagctggcctggagcaggtccacctggccctgaaggcgcagtgctccacg
gaggacgtggactcgctggtggctcagctgacggatgagctcatcgcaggctgcagccag
cctctgggtgacatccatgagacaccctctgccacccacctggaccagtacctgtaccag
ctgcgcacccatcacctgagccaaatcactgaggctgccctggccctgaagctcggccac
agtgagctccctgcagctctggagcaggcggaggactggctcctgcgtctgcgtgccctg
gcagaggagccccagaacagcctgccggacatcgtcatctggatgctgcagggagacaag
cgtgtggcataccagcgggtgcccgcccaccaagtcctcttctcccggcggggtgccaac
tactgtggcaagaattgtgggaagctacagacaatctttctgaaatatccgatggagaag
gtgcctggcgcccggatgccagtgcagatacgggtcaagctgtggtttgggctctcagtg
gatgagaaggagttcaaccagtttgctgaggggaagctgtctgtctttgctgaaacctat
gagaacgagactaagttggcccttgttgggaactggggcacaacgggcctcacctacccc
aagttttctgacgtcacgggcaagatcaagctacccaaggacagcttccgcccctcggcc
ggctggacctgggctggagattggttcgtgtgtccggagaagactctgctccatgacatg
gacgccggtcacctgagcttcgtggaagaggtgtttgagaaccagacccggcttcccgga
ggccagtggatctacatgagtgacaactacaccgatgtgaacggggagaaggtgcttccc
aaggatgacattgagtgcccactgggctggaagtgggaagatgaggaatggtccacagac
ctcaaccgggctgtcgatgagcaaggctgggagtatagcatcaccatccccccggagcgg
aagccgaagcactgggtccctgctgagaagatgtactacacacaccgacggcggcgctgg
gtgcgcctgcgcaggagggatctcagccaaatggaagcactgaaaaggcacaggcaggcg
gaggcggagggcgagggctgggagtacgcctctctttttggctggaagttccacctcgag
taccgcaagacagatgccttccgccgccgccgctggcgccgtcgcatggagccactggag
aagacggggcctgcagctgtgtttgcccttgagggggccctgggcggcgtgatggatgac
aagagtgaagattccatgtccgtctccaccttgagcttcggtgtgaacagacccacgatt
tcctgcatattcgactatgggaaccgctaccatctacgctgctacatgtaccaggcccgg
gacctggctgcgatggacaaggactctttttctgatccctatgccatcgtctccttcctg
caccagagccagaagacggtggtggtgaagaacacccttaaccccacctgggaccagacg
ctcatcttctacgagatcgagatctttggcgagccggccacagttgctgagcaaccgccc
agcattgtggtggagctgtacgaccatgacacttatggtgcagacgagtttatgggtcgc
tgcatctgtcaaccgagtctggaacggatgccacggctggcctggttcccactgacgagg
ggcagccagccgtcgggggagctgctggcctcttttgagctcatccagagagagaagccg
gccatccaccatattcctggttttgaggtgcaggagacatcaaggatcctggatgagtct
gaggacacagacctgccctacccaccaccccagagggaggccaacatctacatggttcct
cagaacatcaagccagcgctccagcgtaccgccatcgagatcctggcatggggcctgcgg
aacatgaagagttaccagctggccaacatctcctcccccagcctcgtggtagagtgtggg
ggccagacggtgcagtcctgtgtcatcaggaacctccggaagaaccccaactttgacatc
tgcaccctcttcatggaagtgatgctgcccagggaggagctctactgcccccccatcacc
gtcaaggtcatcgataaccgccagtttggccgccggcctgtggtgggccagtgtaccatc
cgctccctggagagcttcctgtgtgacccctactcggcggagagtccatccccacagggt
ggcccagacgatgtgagcctactcagtcctggggaagacgtgctcatcgacattgatgac
aaggagcccctcatccccatccagcttgcagacggtctgtcgagcttggcccccactaac
acggcttctcctccatccagtcctcatgaggaagagttcatcgattggtggagcaaattc
tttgcctccataggggagagggaaaagtgcggctcctacctggagaaggattttgacacc
ctgaaggtctatgacacacagctggagaatgtggaggcctttgagggcctgtctgacttt
tgtaacaccttcaagctgtaccggggcaagacgcaggaggagacagaagatccatctgtg
attggtgaatttaagggcctcttcaaaatttatcccctcccagaagacccagccatcccc
atgcccccaagacagttccaccagctggccgcccagggaccccaggagtgcttggtccgt
atctacattgtccgagcatttggcctgcagcccaaggaccccaatggaaagtgtgatcct
tacatcaagatctccatagggaagaaatcagtgagtgaccaggataactacatcccctgc
acgctggagcccgtatttggaaagatgttcgagctgacctgcactctgcctctggagaag
gacctaaagatcactctctatgactatgacctcctctccaaggacgaaaagatcggtgag
acggtcgtcgacctggagaacaggctgctgtccaagtttggggctcgctgtggactccca
cagacctactgtgtctctggaccgaaccagtggcgggaccagctccgcccctcccagctc
ctccacctcttctgccagcagcatagagtcaaggcacctgtgtaccggacagaccgtgta
atgtttcaggataaagaatattccattgaagagatagaggctggcaggatcccaaaccca
cacctgggcccagtggaggagcgtctggctctgcatgtgcttcagcagcagggcctggtc
ccggagcacgtggagtcacggcccctctacagccccctgcagccagacatcgagcagggg
aagctgcagatgtgggtcgacctatttccgaaggccctggggcggcctggacctcccttc
aacatcaccccacggagagccagaaggtttttcctgcgttgtattatctggaataccaga
gatgtgatcctggatgacctgagcctcacgggggagaagatgagcgacatttatgtgaaa
ggttggatgattggctttgaagaacacaagcaaaagacagacgtgcattatcgttccctg
ggaggtgaaggcaacttcaactggaggttcattttccccttcgactacctgccagctgag
caagtctgtaccattgccaagaaggatgccttctggaggctggacaagactgagagcaaa
atcccagcacgagtggtgttccagatctgggacaatgacaagttctcctttgatgatttt
ctgggctccctgcagctcgatctcaaccgcatgcccaagccagccaagacagccaagaag
tgctccttggaccagctggatgatgctttccacccagaatggtttgtgtccctttttgag
cagaaaacagtgaagggctggtggccctgtgtagcagaagagggtgagaagaaaatactg
gcgggcaagctggaaatgaccttggagattgtagcagagagtgagcatgaggagcggcct
gctggccagggccgggatgagcccaacatgaaccctaagcttgaggacccaaggcgcccc
gacacctccttcctgtggtttacctccccatacaagaccatgaagttcatcctgtggcgg
cgtttccggtgggccatcatcctcttcatcatcctcttcatcctgctgctgttcctggcc
atcttcatctacgccttcccgaactatgctgccatgaagctggtgaagcccttcagctga
|
蛋白序列编号: | NP_001124459.1 |
蛋白描述: | dysferlin isoform 1 [Homo sapiens] |
蛋白大小: | 2119 aa |
蛋白序列: |
MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPL
DQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVL
QVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTMDTRYSGKKW
PAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPT
SRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFF
NLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDD
FSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRA
EDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITL
PAMFPSMCEKMRIRIIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCY
INLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILR
VEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVF
DGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCST
EDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGH
SELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGAN
YCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETY
ENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDM
DAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTD
LNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQA
EAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDD
KSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFL
HQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGR
CICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDES
EDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECG
GQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTI
RSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTN
TASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDF
CNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVR
IYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEK
DLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQL
LHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLV
PEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTR
DVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAE
QVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKK
CSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERP
AGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLA
IFIYAFPNYAAMKLVKPFS
|
cDNA克隆和表达质粒购买
货号 | 载体 | 片段 | 标签 | 表达系统 | 价格 |
---|---|---|---|---|---|
NM001130987-1 | pOTB7: | 全长 | 无 | cDNA克隆 | 1000 |
NM001130987-2 | pET-28a | 全长 | N-His | 大肠 | 2800 |
NM001130987-3 | pET-21b | 全长 | C-His | 大肠 | 2800 |
NM001130987-4 | pET-32a | 全长 | N-His、N-Trx | 大肠 | 2800 |
NM001130987-5 | pGEX-4T-1 | 全长 | N-GST | 大肠 | 2800 |
NM001130987-6 | pcDNA3.1(+) | 全长 | 无 | 哺乳细胞 | 2800 |
NM001130987-7 | pcDNA3.1/His A | 全长 | N-His | 哺乳细胞 | 2800 |
NM001130987-8 | pcDNA3.1/MycHisA | 全长 | C-His、C-Myc | 哺乳细胞 | 2800 |
NM001130987-9 | pCMV-Tag 2A | 全长 | N-Flag | 哺乳细胞 | 2800 |
NM001130987-10 | pCMV-Tag 4A | 全长 | C-Flag | 哺乳细胞 | 2800 |
NM001130987-11 | pCMV-HA | 全长 | N-HA | 哺乳细胞 | 2800 |
NM001130987-12 | pEGFP-C1 | 全长 | N-EGFP | 哺乳细胞 | 2800 |
NM001130987-13 | pEGFP-N1 | 全长 | C-EGFP | 哺乳细胞 | 2800 |
NM001130987-14 | p3xFlag-CMV-10 | 全长 | N-3xFlag | 哺乳细胞 | 2800 |
NM001130987-15 | p3xFlag-CMV-14 | 全长 | C-3xflag | 哺乳细胞 | 2800 |
NM001130987-16 | pLVX-Puro | 全长 | 无 | 慢病毒 | 2800 |
NM001130987-17 | pLVX-AcGFP1-N1 | 全长 | C-AcGFP | 慢病毒 | 2800 |
NM001130987-18 | pLVX-AcGFP1-C1 | 全长 | N-AcGFP | 慢病毒 | 2800 |
NM001130987-19 | pLVX-IRES-zsGreen | 全长 | 无 | 慢病毒 | 2800 |
NM001130987-20 | pFastbacI | 全长 | 无 | 昆虫细胞 | 2800 |
NM001130987-21 | pFastbac-HTB | 全长 | N-His | 昆虫细胞 | 2800 |
NM001130987-22 | pFastbac-CHis | 全长 | C-His | 昆虫细胞 | 2800 |
NM001130987-23 | pFastbac-GST | 全长 | N-GST | 昆虫细胞 | 2800 |
蛋白购买
基因名称 | 表达系统 | 片段 | 标签 | 产品规格 | 价格 |
---|---|---|---|---|---|
DYSF | 大肠 | 全长 | N-His | 100 ug | 3500 |
DYSF | 哺乳 | 全长 | N-His | 100 ug | 5500 |
DYSF | 昆虫 | 全长 | N-His | 100 ug | 5500 |
抗体购买
产品名称 | 品牌 | 价格 |
---|---|---|
DYSF抗体/ DYSF抗体 | abcam | 询价 |
DYSF抗体/ DYSF抗体 | CST | 询价 |
DYSF抗体/ DYSF抗体 | Santa Cruz | 询价 |