| 基因名称: | WNK1 |
|---|---|
| 基因又名: | HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1; |
| Gene ID: | 65125 |
| 种属 | Homo sapiens |
| DNA序列编号: | NM_018979.4 |
| DNA描述 | Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 1, mRNA |
| DNA大小: | 10796 bp |
| DNA编码区: |
atgtctggcggcgccgcagagaagcagagcagcactcccggttccctgttcctctcgccg
ccggctcctgcccccaagaatggctccagctccgattcctccgtgggggagaaactggga
gccgcggccgccgacgctgtgaccggcaggaccgaggagtacaggcgccgccgccacact
atggacaaggacagccgtggggcggccgcgaccactaccaccactgagcaccgcttcttc
cgccggagcgtcatctgtgactccaatgccactgcactggagcttcccggccttcctctt
tccctgccccagcccagcatccccgcggctgtcccgcagagtgctccaccggagccccac
cgggaagagaccgtgaccgccaccgccacttcccaggtagcccagcagcctccagccgct
gccgcccctggggaacaggccgtcgcgggccctgccccctcgactgtccccagcagtacc
agcaaagaccgcccagtgtcccagcctagccttgtggggagcaaagaggagccgccgccg
gcgagaagtggcagcggcggcggcagcgccaaggagccacaggaggaacggagccagcag
caggatgatatcgaagagctggagaccaaggccgtgggaatgtctaacgatggccgcttt
ctcaagtttgacatcgaaatcggcagaggctcctttaagacggtctacaaaggtctggac
actgaaaccaccgtggaagtcgcctggtgtgaactgcaggatcgaaaattaacaaagtct
gagaggcagagatttaaagaagaagctgaaatgttaaaaggtcttcagcatcccaatatt
gttagattttatgattcctgggaatccacagtaaaaggaaagaagtgcattgttttggtg
actgaacttatgacgtctggaacacttaaaacgtatctgaaaaggtttaaagtgatgaag
atcaaagttctaagaagctggtgccgtcagatccttaaaggtcttcagtttcttcatact
cgaactccacctatcattcaccgcgatcttaaatgtgacaacatctttatcaccggccct
actggctcagtcaagattggagacctcggtctggcaaccctgaagcgggcttcttttgcc
aagagtgtgataggtaccccagagttcatggcccctgagatgtatgaggagaaatatgat
gaatccgttgacgtttatgcttttgggatgtgcatgcttgagatggctacatctgaatat
ccttactcggagtgccaaaatgctgcacagatctaccgtcgcgtgaccagtggggtgaag
ccagccagttttgacaaagtagcaattcctgaagtgaaggaaattattgaaggatgcata
cgacaaaacaaagatgaaagatattccatcaaagaccttttgaaccatgccttcttccaa
gaggaaacaggagtacgggtagaattagcagaagaagatgatggagaaaaaatagccata
aaattatggctacgtattgaagatattaagaaattaaagggaaaatacaaagataatgaa
gctattgagttttcttttgatttagagagagatgtcccagaagatgttgcacaagaaatg
gtagagtctgggtatgtctgtgaaggtgatcacaagaccatggctaaagctatcaaagac
agagtatcattaattaagaggaaacgagagcagcggcagttggtacgggaggagcaagaa
aaaaaaaagcaggaagagagcagtctcaaacagcaggtagaacaatccagtgcttcccag
acaggaatcaagcagctcccttctgctagcaccggcatacctactgcttctaccacttca
gcttcagtttctacacaagtagaacctgaagaacctgaggcagatcaacatcaacaacta
cagtaccagcaacccagtatatctgtgttatctgatgggacggttgacagtggtcaggga
tcctctgtcttcacagaatctcgagtgagcagccaacagacagtttcatatggttcccaa
catgaacaggcacattctacaggcacagtcccagggcatataccttctactgtccaagca
cagtctcagccccatggggtatatccaccctcaagtgtggcacaggggcagagccagggt
cagccatcctcaagtagcttaacaggggtttcatcttcccaacccatacaacatcctcag
cagcagcagggaatacagcagacagcccctcctcaacagacagtgcagtattcactttca
cagacatcaacctccagtgaggccactactgcacagccagtgagtcagcctcaagctcca
caagtcttgcctcaagtatcagctggaaaacagcttccagtttcccagccagtaccaact
atccaaggcgaacctcagatcccagttgcgacacaaccctcggttgttccagtccactct
ggtgctcatttccttccagtgggacagccgctccctactcccttgctccctcagtaccct
gtctctcagattcccatatcaactcctcatgtgtctacggctcagacaggtttctcatcc
cttcccatcacaatggcagctggcattactcagcctctgctcacgttggcttcatctgct
acaacagctgcgatcccgggggtatcaactgtggttcctagtcagcttccaacccttctg
cagcctgtgactcagctgccaagtcaggttcacccacagctcctacaaccagcagttcag
tccatgggaataccagctaaccttggacaagctgctgaggttccactttcctctggagat
gttctgtaccagggcttcccacctcgactgccaccacagtacccaggagattcaaatatt
gctccctcttccaacgtggcttctgtttgcatccattctacagtcctatcccctcccatg
ccgacagaagtactggctacacctgggtactttcccacagtggtgcagccttatgtggaa
tcaaatcttttagttcctatgggtggtgtaggaggacaggttcaagtgtcccagccagga
gggagtttagcacaagcccccactacatcctcccagcaagcagttttggagagtactcag
ggagtctctcaggttgctcctgcagagccagttgcagtagcacagacccaagctacccag
ccgaccactttggcttcctctgtagacagtgcacattcagatgttgcttcaggtatgagt
gatggcaatgagaacgtcccatcttccagtggaaggcatgaaggaagaactacaaaacgg
cattaccgaaaatctgtaaggagtcgctctcgacatgaaaaaacttcacgcccaaaatta
agaattttgaatgtttcaaataaaggagaccgagtagtagaatgtcaattagagactcat
aataggaaaatggttacattcaaatttgacctagatggtgacaaccccgaggagatagca
acaattatggtgaacaatgactttattctagcaatagagagagagtcgtttgtggatcaa
gtgcgagaaattattgaaaaagctgatgaaatgctcagtgaggatgtcagtgtggaacca
gagggtgatcagggattggagagtctacaaggaaaggatgactatggcttttcaggttct
cagaaattggaaggagagttcaaacaaccaattcctgcgtcttccatgccacagcaaata
ggcattcctaccagttctttaactcaagttgttcattctgcgggaaggcggtttatagtg
agtcctgtgccagaaagccgattacgagaatcaaaagttttccccagtgaaataacagat
acagttgctgcctctacagctcagagccctggaatgaacttgtctcactctgcatcatcc
cttagtctacaacaggccttttctgaacttagacgtgcccaaatgacagaaggacccaac
acagcacctccaaactttagtcatacaggaccaacatttccagtagtacctcctttctta
agtagcattgctggagtcccaaccacagcagcagccacagcaccagtccctgcaacaagc
agccctcctaatgacatttccacatcagtaattcagtctgaggttacagtgcccactgaa
gaggggattgctggagttgccaccagcacaggtgtggtaacttcaggtggtctccccata
ccacctgtgtctgaatcaccagtactttccagcgtagtttcaagtatcacaatacctgca
gttgtctcaatatctactacatccccgtcacttcaagtccccacatccacatctgagatc
gttgtttctagtacagcactgtatccttcagtaacagtttcagcaacttcagcctctgca
gggggcagtactgctaccccaggtcctaagcctccagctgtagtatctcagcaggcagca
ggcagcactactgtgggagccacattaacatcagtttctaccaccacttcattcccaagc
acagcttcacagctgtgcattcagcttagcagcagtacttctactcctactttagctgaa
accgtggtagttagcgcacactcactagataagacatctcatagcagtacaactggattg
gctttctccctctctgcaccatcttcctcttcctctcctggagcaggagtgtctagttat
atttctcagcctggtgggctgcatcctttggtcattccatcagtgatagcttctactcct
attcttccccaagcagcaggacctacttctacacctttattaccccaagtacctagtatc
ccacccttggtacagcctgttgccaatgtgcctgctgtacagcagacactaattcatagt
cagcctcaaccagctttgcttcccaaccagccccatactcattgtcctgaagtagattct
gatacacaacccaaagctcctggaattgatgacataaagactctagaagaaaagctgcgg
tctctgttcagtgaacacagctcatctggagctcagcatgcctctgtctcactggagacc
tcactagtcatagagagcactgtcacaccaggcatcccaactactgctgttgcaccaagc
aaactcctgacttctaccacaagtacttgcttaccaccaaccaatttaccactaggaaca
gttgctttgccagttacaccagtggtcacacctgggcaagtttctaccccagtcagcact
actacatcaggagtgaaacctggaactgctccctccaagccacctctaactaaggctccg
gtgctgccagtgggtactgaacttccagcaggtactctacccagcgagcagctgccacct
tttccaggaccttctctaacccagtcccagcaacctctagaggatcttgatgctcaattg
agaagaacacttagtccagagatgatcacagtgacttctgcggttggtcctgtgtccatg
gcggctccaacagcaatcacagaagcaggaacacagcctcagaagggtgtttctcaagtc
aaagaaggccctgtcctagcaactagttcaggagctggtgtttttaagatgggacgattt
caggtttctgttgcagcagacggtgcccagaaagagggtaaaaataagtcagaagatgca
aagtctgttcattttgaatccagcacctcagagtcctcagtgctatcaagtagtagtcca
gagagtaccttggtgaaaccagagccgaatggcataaccatccctggtatctcttcagat
gtgccagagagtgcccacaaaactactgcctcagaggcaaagtcagacactgggcagcct
accaaggttggacgttttcaggtgacaactacagcaaacaaagtgggtcgtttctctgta
tcaaaaactgaggacaagatcactgacacaaagaaagaaggaccagtggcatctcctcct
tttatggatttggaacaagctgttcttcctgctgtgataccaaagaaagagaagcctgaa
ctgtcagagccttcacatctaaatgggccgtcttctgacccggaggccgcttttttaagt
agggatgtggatgatggttccggtagtccacactcgccccatcagctgagctcaaagagc
cttcctagccagaatctaagtcaaagccttagtaattcatttaactcctcttacatgagt
agcgacaatgagtcagatatcgaagatgaagacttaaagttagagctgcgacgactacga
gataaacatctcaaagagattcaggacctgcagagtcgccagaagcatgaaattgaatct
ttgtataccaaactgggcaaggtgccccctgctgttattattcccccagctgctcccctt
tcagggagaagacgacgacccactaaaagcaaaggcagcaaatctagtcgaagcagttcc
ttggggaataaaagcccccagctttcaggtaacctgtctggtcagagtgcagcttcagtc
ttgcacccccagcagaccctccaccctcctggcaacatcccagagtccgggcagaatcag
ctgttacagccccttaagccatctccctccagtgacaacctctattcagccttcaccagt
gatggtgccatttcagtaccaagcctttctgctccaggtcaaggaaccagcagcacaaac
actgttggggcaacagtgaacagccaagccgcccaagctcagcctcctgccatgacgtcc
agcaggaagggcacattcacagatgacttgcacaagttggtagacaattgggcccgagat
gccatgaatctctcaggcaggagaggaagcaaagggcacatgaattacgagggccctgga
atggcaaggaagttctctgcacctgggcaactgtgcatctccatgacctcgaacctgggt
ggctctgcccccatctctgcagcatcagctacctctctaggtcacttcaccaagtctatg
tgccccccacagcagtatggctttccagctaccccatttggcgctcaatggagtgggacg
ggtggcccagcaccacagccacttggccagttccaacctgtgggaactgcctccttgcag
aatttcaacatcagcaatttgcagaaatccatcagcaaccccccaggctccaacctgcgg
accacttag
|
| 蛋白序列编号: | NP_061852.3 |
| 蛋白描述: | serine/threonine-protein kinase WNK1 isoform 1 [Homo sapiens] |
| 蛋白大小: | 2382 aa |
| 蛋白序列: |
MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHT
MDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPH
REETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPP
ARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLD
TETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLV
TELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGP
TGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEY
PYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQ
EETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEM
VESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQ
TGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQG
SSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQG
QPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAP
QVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYP
VSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLL
QPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNI
APSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPG
GSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMS
DGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETH
NRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEP
EGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIV
SPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPN
TAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTE
EGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEI
VVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPS
TASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSY
ISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHS
QPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLET
SLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVST
TTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQL
RRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRF
QVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSD
VPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPP
FMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKS
LPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIES
LYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASV
LHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTN
TVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPG
MARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGT
GGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT
|
cDNA克隆和表达质粒购买
| 货号 | 载体 | 片段 | 标签 | 表达系统 | 价格 |
|---|---|---|---|---|---|
| NM018979-1 | pOTB7: | 全长 | 无 | cDNA克隆 | 1000 |
| NM018979-2 | pET-28a | 全长 | N-His | 大肠 | 2800 |
| NM018979-3 | pET-21b | 全长 | C-His | 大肠 | 2800 |
| NM018979-4 | pET-32a | 全长 | N-His、N-Trx | 大肠 | 2800 |
| NM018979-5 | pGEX-4T-1 | 全长 | N-GST | 大肠 | 2800 |
| NM018979-6 | pcDNA3.1(+) | 全长 | 无 | 哺乳细胞 | 2800 |
| NM018979-7 | pcDNA3.1/His A | 全长 | N-His | 哺乳细胞 | 2800 |
| NM018979-8 | pcDNA3.1/MycHisA | 全长 | C-His、C-Myc | 哺乳细胞 | 2800 |
| NM018979-9 | pCMV-Tag 2A | 全长 | N-Flag | 哺乳细胞 | 2800 |
| NM018979-10 | pCMV-Tag 4A | 全长 | C-Flag | 哺乳细胞 | 2800 |
| NM018979-11 | pCMV-HA | 全长 | N-HA | 哺乳细胞 | 2800 |
| NM018979-12 | pEGFP-C1 | 全长 | N-EGFP | 哺乳细胞 | 2800 |
| NM018979-13 | pEGFP-N1 | 全长 | C-EGFP | 哺乳细胞 | 2800 |
| NM018979-14 | p3xFlag-CMV-10 | 全长 | N-3xFlag | 哺乳细胞 | 2800 |
| NM018979-15 | p3xFlag-CMV-14 | 全长 | C-3xflag | 哺乳细胞 | 2800 |
| NM018979-16 | pLVX-Puro | 全长 | 无 | 慢病毒 | 2800 |
| NM018979-17 | pLVX-AcGFP1-N1 | 全长 | C-AcGFP | 慢病毒 | 2800 |
| NM018979-18 | pLVX-AcGFP1-C1 | 全长 | N-AcGFP | 慢病毒 | 2800 |
| NM018979-19 | pLVX-IRES-zsGreen | 全长 | 无 | 慢病毒 | 2800 |
| NM018979-20 | pFastbacI | 全长 | 无 | 昆虫细胞 | 2800 |
| NM018979-21 | pFastbac-HTB | 全长 | N-His | 昆虫细胞 | 2800 |
| NM018979-22 | pFastbac-CHis | 全长 | C-His | 昆虫细胞 | 2800 |
| NM018979-23 | pFastbac-GST | 全长 | N-GST | 昆虫细胞 | 2800 |
蛋白购买
| 基因名称 | 表达系统 | 片段 | 标签 | 产品规格 | 价格 |
|---|---|---|---|---|---|
| WNK1 | 大肠 | 全长 | N-His | 100 ug | 3500 |
| WNK1 | 哺乳 | 全长 | N-His | 100 ug | 5500 |
| WNK1 | 昆虫 | 全长 | N-His | 100 ug | 5500 |
抗体购买
| 产品名称 | 品牌 | 价格 |
|---|---|---|
| WNK1抗体/ WNK1抗体 | abcam | 询价 |
| WNK1抗体/ WNK1抗体 | CST | 询价 |
| WNK1抗体/ WNK1抗体 | Santa Cruz | 询价 |
