保藏机构: | JCM |
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菌种名称: | JCM4926, JCM 4926 |
英文名称: | Prevotella sp. |
中文名称 | 普雷沃菌 |
存储人: | |
来源历史: | << Huang and Liou, gm001 |
其他保藏中心编号: | JCM33280 |
分离来源: | 人类粪便 |
菌种特性 | |
备注: | |
培养条件: | 37°C |
生物安全等级: | 1 |
氧气需求: | |
培养基: |
TSB培养基:72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar 15.0 g
Defibrinated Sheep Blood 50.0 mL
Distilled water 1.0 L
NaCl 5.0 g
Soytone 5.0 g
Tryptone 15.0 g
983
YCFA GSC
Glucose 0.2 g
Maltose 0.2 g
Cellobiose 0.2 g
Min I 15 mL
Min II 15 mL
VFA mix 0.31 mL
Haemin solution 1 mL
Vitamin solution 1 100.00 μL
Vitamin solution 2 100.00 μL
Resazurine 0.1 mL
L-cysteine 0.1 g
Distilled water up to 100 mL
Agar (if need) 1.5 g
Bacto casitone 1 g
Yeast extract 0.25 g
Boil medium gently while adding ingredients except Cysteine HCl and Vitamin solution 2. Add the cysteine last after the medium has been cooled by bubbling oxygen free CO2 through it. When the medium has cooled to below 60°C, the pH is checked and adjusted if required. The complete medium is dispensed into appropiate vessels under a CO2 blanket, immediately sealed and autoclaved.
VFA mix
Acetic acid 17 mL
Propionic acid 6 mL
n-Valeric acid 1 mL
Isovaleric acid 1 mL
Isobutyric acid 1 mL
The VFA mix does not contain butyric acid for the purposes of the VFA analysis. The formula of the haemin and vitamin solutions are explained below.
Haemin solution
KOH 0.28 g
Eathanol 95% 25 mL
Haemin 100 mg
Distilled water up to 100 mL
Vatamin solution 1
Biotin 1 mg
Cobalamin 1 mg
PABA 3 mg
Folic acid 5 mg
Pyridoxamine 15 mg
Distilled water up to 100 mL
Vitamin solution 2
Thiamin 5 mg
Riboflavin 5 mg
Distilled water up to 100 mL
Vitamin solution 2 is added to the medium after autoclaving as a filtered-sterilised solution. The pH of the medium is adjusted to 7.3 with NaOH before autoclaving and it will drop to about 6.8 after autoclaving.
Mineral I
K2HPO4 3 g
Distilled water 1 L
Mineral II
KH2PO4 3 g
(NH4)2SO4 6 g
NaCl 6 g
MgSO4 0.6 g
CaCl2(dry) 0.6 g
Distilled water 1 L
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模式菌株: | 是 |
参考文献: |
JCM33280
价格:3200元
代购价格:询价
联系方式:I47-825O-882O