JCM33280

价格:3200元

代购价格:询价

联系方式:I47-825O-882O

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JCM33280标准菌株基本信息

保藏机构: JCM
菌种名称: JCM4926, JCM 4926
英文名称: Prevotella sp.
中文名称 普雷沃菌
存储人:  
来源历史: << Huang and Liou, gm001
其他保藏中心编号: JCM33280
分离来源: 人类粪便
菌种特性  
备注:  
培养条件: 37°C
生物安全等级: 1
氧气需求:  
培养基: TSB培养基:72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar 15.0 g
Defibrinated Sheep Blood 50.0 mL
Distilled water 1.0 L
NaCl 5.0 g
Soytone 5.0 g
Tryptone 15.0 g
983
YCFA GSC
Glucose 0.2 g
Maltose 0.2 g
Cellobiose 0.2 g
Min I 15 mL
Min II 15 mL
VFA mix 0.31 mL
Haemin solution 1 mL
Vitamin solution 1 100.00 μL
Vitamin solution 2 100.00 μL
Resazurine 0.1 mL
L-cysteine 0.1 g
Distilled water up to 100 mL
Agar (if need) 1.5 g
Bacto casitone 1 g
Yeast extract 0.25 g
Boil medium gently while adding ingredients except Cysteine HCl and Vitamin solution 2. Add the cysteine last after the medium has been cooled by bubbling oxygen free CO2 through it. When the medium has cooled to below 60°C, the pH is checked and adjusted if required. The complete medium is dispensed into appropiate vessels under a CO2 blanket, immediately sealed and autoclaved.
VFA mix
Acetic acid 17 mL
Propionic acid 6 mL
n-Valeric acid 1 mL
Isovaleric acid 1 mL
Isobutyric acid 1 mL
The VFA mix does not contain butyric acid for the purposes of the VFA analysis. The formula of the haemin and vitamin solutions are explained below.
 
Haemin solution
KOH 0.28 g
Eathanol 95% 25 mL
Haemin 100 mg
Distilled water up to 100 mL
 
Vatamin solution 1
Biotin 1 mg
Cobalamin 1 mg
PABA 3 mg
Folic acid 5 mg
Pyridoxamine 15 mg
Distilled water up to 100 mL
 
Vitamin solution 2
Thiamin 5 mg
Riboflavin 5 mg
Distilled water up to 100 mL
Vitamin solution 2 is added to the medium after autoclaving as a filtered-sterilised solution. The pH of the medium is adjusted to 7.3 with NaOH before autoclaving and it will drop to about 6.8 after autoclaving.
 
Mineral I
K2HPO4 3 g
Distilled water 1 L
 
Mineral II
KH2PO4 3 g
(NH4)2SO4 6 g
NaCl 6 g
MgSO4 0.6 g
CaCl2(dry) 0.6 g
Distilled water 1 L
模式菌株:
参考文献:  

常用微生物培养基推荐(可定制)


JCM33280标准菌株说明书pdf版和相关资料下载

JCM33280标准菌株应用举例

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