| 产品名称： | 寄生虫细胞培养基；ATCC培养基 2222 |
|---|---|
| 英文名称： | Cell cultivation medium for parasites；ATCC medium 2222 |
| 培养基类型： | 加富培养基 |
| 级别： | for microbiology |
| 品牌： | ELITE-MEDIA（艾礼培养基） |
| 产品目录号： | M2854 |
| 产品规格： | 250g、500g |
| 产品外观： | 乳白色至浅黄色粉末。 |
| 灭菌后颜色与澄清度： | 浅琥珀色，透明，无沉淀。 |
| 保存条件： | 密封，2-25°C保存。 |
| 注意事项： | 避免摄入、吸入、皮肤接触。 |
| 相关产品： | -- |
产品描述：
胰蛋白胨大豆肉汤（TSB）培养基是加富培养基，用于营养需求高的微生物（难养菌）的培养和回收，不需要加血清。胰蛋白胨大豆肉汤培养基适用范围广泛，可用于培养好氧菌、厌氧菌和真菌。例如：
Bacillus subtilis 枯草芽孢杆菌
Bacteroides vulgatus 普通拟杆菌
Candida albicans 白色念珠菌
Escherichia coli 大肠杆菌
Micrococcus luteus 藤黄微球菌
Staphylococcus aureus 金黄色葡萄球菌
Staphylococcus epidermidis 表皮葡萄球菌
Streptococcus pneumonia 肺炎链球菌
Streptococcus pyogenes 酿脓链球菌
用途：
作为致病菌的富集培养基。用于配制血液培养基和血琼脂培养基。
用于无菌试验，检测真菌和好氧菌污染。
在医学上，常用来培养从临床样品中分离的致病微生物。
用于药敏试验和纸片扩散法试验。
配方与配制方法
| Dulbecco's Minimal Essential Medium (DMEM)* | 450.0 ml |
| Fetal Bovine Serum (FBS)** | 50.0 ml |
将DMEM和冷却的热灭活血清无菌结合。
*ATCC Cat. #30-2002
**ATCC Cat. #30-2021
*ATCC Cat. #30-2002
**ATCC Cat. #30-2021
TCC测试的胎牛血清（FBS）可作为ATCC Cat#获得30-2020（500毫升）和Cat.#30-2021（100毫升）。ATCC分布式FBS未进行热灭活。
Thaw serum: Remove the serum from frozen storage and place it overnight in a refrigerator at 2C to 6C. Transfer the bottles to a 37C water bath for approximately 5 minutes. Agitate the bottles from time-to-time in order to mix the solutes that tend to concentrate at the bottom of the bottle. Do not keep the serum at 37C any longer than necessary to completely thaw it. Thawing serum in a bath above 40C without mixing may lead to the formation of a precipitate inside the bottle. We don't recommend thawing the serum at a high temperature. After the serum has warmed in the 37C water bath, continue with the heat-inactivation process. Heat-inactivate the FBS: Pre-heat water bath to 56C. There must be sufficient water to immerse the bottle above the level of serum. Mix thawed serum by gentle inversion and place serum bottle in the 56C water bath. (The temperature of the water bath will drop.) When the temperature of the water bath reaches 56C again, continue to heat for a additional 30 minutes. Mix gently every five minutes to insure uniform heating. Remove serum from water bath and cool. To avoid contamination, do not allow the level of the water in the bath to come in contact with the lip of the screw cap. It is best to leave one inch between the serum level in the bottle and the lip of the cap and to fill the water bath to a level just slightly above the level of the serum.
Thaw serum: Remove the serum from frozen storage and place it overnight in a refrigerator at 2C to 6C. Transfer the bottles to a 37C water bath for approximately 5 minutes. Agitate the bottles from time-to-time in order to mix the solutes that tend to concentrate at the bottom of the bottle. Do not keep the serum at 37C any longer than necessary to completely thaw it. Thawing serum in a bath above 40C without mixing may lead to the formation of a precipitate inside the bottle. We don't recommend thawing the serum at a high temperature. After the serum has warmed in the 37C water bath, continue with the heat-inactivation process. Heat-inactivate the FBS: Pre-heat water bath to 56C. There must be sufficient water to immerse the bottle above the level of serum. Mix thawed serum by gentle inversion and place serum bottle in the 56C water bath. (The temperature of the water bath will drop.) When the temperature of the water bath reaches 56C again, continue to heat for a additional 30 minutes. Mix gently every five minutes to insure uniform heating. Remove serum from water bath and cool. To avoid contamination, do not allow the level of the water in the bath to come in contact with the lip of the screw cap. It is best to leave one inch between the serum level in the bottle and the lip of the cap and to fill the water bath to a level just slightly above the level of the serum.
