载体序列 内容：
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LOCUS DQ058725 4985 bp DNA circular SYN 08-MAY-2006
DEFINITION Broad host range vector pBMTB-2, complete sequence.
ACCESSION DQ058725
TITLE Broad host range vectors for stable genomic library construction
AUTHORS Lynch,M.D. and Gill,R.T.
JOURNAL Submitted (12-MAY-2005) Chemical & Biological Engineering,
University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
80309, USA
FEATURES Location/Qualifiers
source 1..4985
/organism="Broad host range vector pBMTB-2"
/mol_type="other DNA"
/db_xref="taxon:334957"
regulatory 60..96
/regulatory_class="terminator"
/note="SoxR terminator"
gene complement(104..1123)
/gene="mob"
/note="mob gene from pBBR1MCS, GenBank Accession Number
U02374"
CDS complement(104..1123)
/gene="mob"
/note="required for mobilization"
/codon_start=1
/transl_table=11
/product="Mob"
/protein_id="AAY99701.1"
/db_xref="GI:69048177"
/translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
KPLLS"
gene 2117..2779
/gene="rep"
/note="rep gene from pBBR1, GenBank Accession Number
X66730"
CDS 2117..2779
/gene="rep"
/note="required for replication"
/codon_start=1
/transl_table=11
/product="Rep"
/protein_id="AAY99702.1"
/db_xref="GI:69048178"
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
RR"
gene 2876..3691
/gene="kan"
/note="kanamycin resistance gene from TN903"
CDS 2876..3691
/gene="kan"
/codon_start=1
/transl_table=11
/product="kanamycin kinase"
/protein_id="AAY99703.1"
/db_xref="GI:69048179"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGL
VGASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
regulatory 3708..3739
/regulatory_class="terminator"
/note="TonB terminator"
gene complement(3767..4696)
/gene="araC"
CDS complement(3767..4696)
/gene="araC"
/note="regulator"
/codon_start=1
/transl_table=11
/product="AraC"
/protein_id="AAY99704.1"
/db_xref="GI:69048180"
/translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
regulatory 4704..4971
/regulatory_class="promoter"
/note="pBAD promoter"
ORIGIN
1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt
121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct
181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct
241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa
301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc
361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt
421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt
481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt
541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct
601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct
661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct
721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg
781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga
841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt
901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc
961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt
1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca
1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt
1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg
1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc
1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc
1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt
1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag
1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc
1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc
1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg
1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc
1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt
1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc
1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg
1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa
1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc
1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt
2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta
2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa
2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc
2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa
2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt
2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg
2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga
2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga
2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca
2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc
2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc
2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt
2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc
2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag
2881 ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaaca tggatgctga
2941 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
3001 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
3061 caatgatgtt acagatgaga tggtcaggct aaactggctg acggaattta tgcctcttcc
3121 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
3181 agggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
3241 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
3301 cggcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttgg
3361 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
3421 gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
3481 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
3541 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
3601 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
3661 gtttcacttg atgctcgatg agtttttctg agggcggatc cccctcaagt caaaagcctc
3721 cggtcggagg cttttgactt tctgctatgg aggtcaggta tgatttttat gacaacttga
3781 cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg
3841 tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga
3901 cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt
3961 cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg
4021 acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt
4081 gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact
4141 cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct
4201 ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat
4261 gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt
4321 taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc
4381 gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac
4441 ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga
4501 gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt
4561 tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca
4621 ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca
4681 attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa
4741 ccaaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga
4801 caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc
4861 acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg gatcctacct
4921 gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg gctagagaat
4981 tcgtc
//
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