pEF1α-tdTomato

价格:2300元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pEF1α-tdTomato
质粒类型: 哺乳动物细胞表达载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: EF1α
载体大小: 6149 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 卡那霉素
筛选标记: 新霉素(Neomycin)
克隆菌株: DH5α, HB101
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: pEF1α-tdTomato载体组成型表达tdTomato;
tdTomato基因片段上下游有酶切位点,很容易克隆到其它载体上去。
产品目录号: 631975
稳定性: 稳表达 或 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pEF1α-tdTomato载体图谱



pEF1α-tdTomato 载体特征

载体简介

载体序列

LOCUS       pEF1a-tdTomato   6149 bp    DNA   circular            17-MAR-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631975
FEATURES             Location/Qualifiers
     misc_feature    4045..4839
                     /label=Kan(R)/Neo(R)
     CDS             1385..2812
                     /label=tdTomato
     promoter        12..1346
                     /label=EF1a\Promoter
     polyA_signal    2968..3002
                     /label=SV40\polyA\signal
     rep_origin      complement(3065..3520)
                     /label=f1\origin
     rep_origin      3861..3999
                     /label=SV40\origin
     polyA_signal    5075..5093
                     /label=HSV\TK\polyA\signals
     rep_origin      5424..6067
                     /label=pUC\origin
     misc_feature    1378..1388
                     /label=Kozak\sequence
     misc_feature    3694..3962
                     /label=SV40\early\Promoter
BASE COUNT     1352 a      1725 c      1751 g      1321 t 
ORIGIN
        1 tagttattaa tgagtaattc atacaaaagg actcgcccct gccttgggga atcccaggga 
       61 ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg ccccctcacc 
      121 cgcccgctct cgtcatcact gaggtggaga agagcatgcg tgaggctccg gtgcccgtca 
      181 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 
      241 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 
      301 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 
      361 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg 
      421 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc 
      481 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc 
      541 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggctt gggcgctggg 
      601 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc 
      661 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg 
      721 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg 
      781 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga 
      841 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc 
      901 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg 
      961 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg 
     1021 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg 
     1081 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct 
     1141 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca 
     1201 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat 
     1261 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt 
     1321 tttttcttcc atttcaggtg tcgtgacgct agcgctaccg gactcagatc caccggtcgc 
     1381 caccatggtg agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat 
     1441 ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta 
     1501 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg 
     1561 ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga 
     1621 catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa 
     1681 cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct 
     1741 gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa 
     1801 gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa 
     1861 gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa 
     1921 gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa 
     1981 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga 
     2041 gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg 
     2101 caccgcctcc tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt 
     2161 gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg 
     2221 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt 
     2281 cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc 
     2341 cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt 
     2401 gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg 
     2461 cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat 
     2521 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt 
     2581 gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga 
     2641 gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga 
     2701 caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg 
     2761 ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca agtaggcggc 
     2821 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa 
     2881 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact 
     2941 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 
     3001 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag 
     3061 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc 
     3121 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc 
     3181 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 
     3241 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 
     3301 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg 
     3361 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 
     3421 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 
     3481 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga 
     3541 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 
     3601 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg 
     3661 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 
     3721 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 
     3781 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 
     3841 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     3901 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 
     3961 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa 
     4021 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg 
     4081 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct 
     4141 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac 
     4201 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg 
     4261 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 
     4321 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa 
     4381 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca 
     4441 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt 
     4501 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc 
     4561 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc 
     4621 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg 
     4681 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt 
     4741 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag 
     4801 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa 
     4861 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct 
     4921 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 
     4981 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg 
     5041 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg 
     5101 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata 
     5161 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc 
     5221 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca 
     5281 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 
     5341 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 
     5401 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 
     5461 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 
     5521 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 
     5581 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 
     5641 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 
     5701 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 
     5761 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 
     5821 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 
     5881 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 
     5941 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 
     6001 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 
     6061 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 
     6121 ctgtggataa ccgtattacc gccatgcat 
//

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