TEV protease





出品公司: invitrogen
载体名称: TEV protease
质粒类型: 大肠杆菌表达载体
高拷贝/低拷贝: --
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: --
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: 6xHis
载体抗性: 氨苄青霉素(Ampicillin)
筛选标记: --
备注: --
产品目录号: 12575-015
稳定性: --
组成型: --
病毒/非病毒: --



AcTEV™ Protease specifically recognizes a seven amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly), making it 

useful for removing affinity tags from fusion proteins. AcTEV™ Protease is an improved version of Tobacco Etch Virus (TEV) protease that is 

highly site-specific, highly active, and significantly more stable than native TEV protease, resulting in enhanced long-term activity. AcTEV™ 

Protease features:

• Highly specific cleavage activity
• Enhanced enzyme stability for prolonged protease activity
• Activity over a broad temperature (+4°C to 30°C) and pH (6.0 to 8.5) range
• Six-histidine sequence to facilitate its removal from the digested protein sample
• Greater than 85% single-band purity with no nonspecific protease contaminationApplications 
Incubation with AcTEV™ Protease releases the protein of interest from the fusion tag. This is an effective way to remove solubility, secretion, 

detection, and purification tags from recombinant proteins.Enzyme specifications
Purified from E. coli expressing the AcTEV™ Protease gene.

Unit definition
One unit of AcTEV™ Protease cleaves 85% of a 3 µg control substrate in 1 hr at 30°C.

Unit reaction conditions
50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA, 1 mM DTT, 3 µg control substrate, and 1 unit enzyme in 30 µL for 1 hr at 30°C. AcTEV™ Protease is 

functionally tested for the absence of any nonspecific protease activity.




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