| 保藏机构: | CIP |
|---|---|
| 菌种名称: | CIP109440, CIP 109440 |
| 英文名称: | Hungatella hathewayi |
| 中文名称 | |
| 存储人: | (Steer et al.) Kaur et al. |
| 来源历史: | << DSMZ << H. Hippe << P. A. Lawson << T. Steer; 1313 |
| 其他保藏中心编号: | DSM 13479 ;CCUG 43506 ;CIP 109440 ;MTCC 10951 |
| 分离来源: | 人类粪便 |
| 菌种特性 | |
| 备注： | |
| 培养条件： | 30°C |
| 生物安全等级: | 1 |
| 氧气需求: | 厌氧 |
| 培养基: |
TSB培养基：199
MODIFIED PYG-MEDIUM
Trypticase peptone 5.0 g
Peptone 5.0 g
Yeast extract 10.0 g
Beef extract 5.0 g
Tween 80 1.0 ml
Resazurin 1.0 mg
Salt solution (see below) 40.0 ml
Hemin solution (see below) 10.0 ml
Distilled water 950.0 ml
Glucose 5.0 g
K2HPO4 2.0 g
Vitamin K1 solution (see below) 0.2 ml
Cysteine·HCl 0.5 g
The vitamin K1, hemin solution, and cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.2 with 8N NaOH. Distribute under N2 and autoclave.
Salt solution:
CaCl2·2H2O 0.25 g
MgSO4·7H2O 0.5 g
K2HPO4 1.0 g
KH2PO4 1.0 g
NaHCO3 10.0 g
NaCl 2.0 g
Distilled water 1.0 L
72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar 15.0 g
Defibrinated Sheep Blood 50.0 mL
Distilled water 1.0 L
NaCl 5.0 g
Soytone 5.0 g
Tryptone 15.0 g
|
| 模式菌株: | 是 |
| 参考文献: |
B2298
Steer, T., M. D. Collins, G. R. Gibson, H. Hippe, and P. A. Lawson. 2001. Clostridium hathewayi sp. nov., from human faeces. Syst. Appl. Microbiol. 24 : 353.
B2299
Kaur, S., M. Yawar, P. A. Kumar, and K. Suresh. 2014. Hungatella effluvii gen. nov., sp. nov., an obligately anaerobic bacterium isolated from an effluent treatment plant, and reclassification of Clostridium hathewayi as Hungatella hathewayi gen. nov., comb. nov. 64 : 710.
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