| 保藏机构: | JCM |
|---|---|
| 菌种名称: | JCM4926, JCM 4926 |
| 英文名称: | Lophiostoma winteri |
| 中文名称 | |
| 存储人: | (Saccardo) G. Winter |
| 来源历史: |
<< CBS
|
| 其他保藏中心编号: | CBS 491.96; JCM 14134 |
| 分离来源: | 灌丛 |
| 菌种特性 | |
| 备注： | |
| 培养条件： | 20°C |
| 生物安全等级: | 1 |
| 氧气需求: | 需氧 |
| 培养基: |
TSB培养基：13
OATMEAL AGAR I (ISP MEDIUM 3)
Oatmeal (Quaker white oats) 20.0g
Agar 18.0g
Distilled water 1.0L
Adjust pH to 7.2. Cook or steam 20g of oatmeal in 1.0L distilled water for 20 minutes. Filter through cheesecloth. Add 18g agar and make up to 1.0L. Add 1 ml of trace salts solution (see below).
Trace salts solution:
FeSO4·7H2O 0.1g
MnCl2·4H2O 0.1g
ZnSO4·7H2O 0.1g
Distilled water 100.0ml
163
OATMEAL AGAR IV
Heat 30g oat flakes in 1.0L water till boiling and simmer gently for 2 hours. Filter through cheesecloth and sterilize for 1h at 1 atm. Fill up to 1.0L. When using powdered oatmeal, filtering is superfluous. Lupin stems may be placed in slants with oatmeal agar.
79
CORN MEAL AGAR (DIFCO 0386)
Corn meal, infusion from 50.0g
Agar 15.0g
Distilled water 1.0L
|
| 模式菌株: | 否 |
| 参考文献: |
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