| 出品公司: | Amersham Biosciences/ GE |
|---|---|
| 载体名称: | pCantab 5E, pCantab5E, pCantab-5E |
| 质粒类型: | 重组噬菌体抗体系统, 噬菌体展示系统 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | Lac |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4522 bp |
| 5' 测序引物及序列: | pCantab5-R1: ccatgattacgccaagctttggagcc |
| 3' 测序引物及序列: | pCantab5-R2: cgatctaaagttttgtcgtctttcc |
| 载体标签: | -- |
| 载体抗性: | 氨苄 |
| 筛选标记: | -- |
| 备注: | 由噬菌体展示系统发展而来。 |
| 产品目录号: | 27-9400-01/ 27-9401-01/ 27-9402-01 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | 非病毒 |
pCantab 5E
价格：1500元
联系方式：I47-825O-882O
买家导航
pCantab5E载体基本信息
pCantab5E载体质粒图谱和多克隆位点信息
pCantab5E载体简介
重组噬菌体抗体系统是设计用来克隆小鼠的抗体基因，表达，检测功能性抗体。本载体是基于噬菌体展示系统，将克隆得到的抗体片段表达出来并且在噬菌体表面展示出来。当抗体基因被克隆进入pCantab 5E载体，可溶性的重组抗体也可以作为免疫试剂使用。
pCantab5E载体序列
LOCUS pCANTAB 5E 4591 bp DNA circular SYN 31-OCT-2013
DEFINITION pCANTAB5E.
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
promoter 131..159
/vntifkey="30"
/label=AmpR_promoter
CDS 201..1061
/gene="Ampicillin"
/vntifkey="4"
/label=Ampicillin
/note="ORF frame 3"
gene 201..1061
/gene="Ampicillin"
/vntifkey="60"
rep_origin 1216..1835
/vntifkey="33"
/label=pBR322_origin
promoter 2144..2173
/vntifkey="30"
/label=lac_promoter
misc_feature 2187..2209
/vntifkey="21"
/label=M13_pUC_rev_primer
misc_feature 2208..2226
/vntifkey="21"
/label=M13_reverse_primer
CDS 2218..3713
/vntifkey="4"
/label=ORF\frame\1
misc_feature complement(3723..3739)
/vntifkey="21"
/label=M13_forward20_primer
misc_feature complement(3732..3754)
/vntifkey="21"
/label=M13_pUC_fwd_primer
misc_feature 3720..3875
/vntifkey="21"
/label=lacZ_a
rep_origin complement(4067..4373)
/vntifkey="33"
/label=f1_origin
misc_feature 4541..4563
/vntifkey="21"
/label=pGEX_3_primer
misc_feature 2329..2342
/vntifkey="21"
/label=VH
misc_feature 2437..2441
/vntifkey="21"
/label=VL
misc_feature 2376..2398
/vntifkey="21"
/label=pCantab5-S3
misc_feature complement(2376..2398)
/vntifkey="21"
/label=pCantab5-S4
primer 2222..2247
/vntifkey="27"
/label=pCantab5-R1
primer 2265..2288
/vntifkey="27"
/label=pCantab5-S1
misc_feature complement(2523..2545)
/vntifkey="21"
/label=pCantab-S6
misc_feature complement(2554..2578)
/vntifkey="21"
/label=pCantab-R2
misc_feature 2451..2489
/vntifkey="21"
/label=E\Tag
misc_feature 2496..2498
/vntifkey="21"
/label=Amber\Stop\Codon
BASE COUNT 1112 a 1099 c 1172 g 1196 t 12 others
ORIGIN
1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
301 ctgaagatca gttgggtgct cgagtgggtt acatcgaact ggatctcaac agcggtaaga
361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
1561 cgtgcataca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
2221 accatgatta cgccaagctt tggagccttt tttttggaga ttttcaacgt gaaaaaatta
2281 ttattcgcaa ttcctttagt tgttcctttc tatgcggccc agccggccnn nnnnggggcc
2341 aaggcaccac ggtcaccgtc tcctcaggtg gaggcggttc aggcggaggt ggctctggcg
2401 gtggcggatc ggacatcgag ctcactcagt ctccannnnn ngcggccgca ggtgcgccgg
2461 tgccgtatcc ggatccgctg gaaccgcgtg ccgcatagac tgttgaaagt tgtttagcaa
2521 aacctcatac agaaaattca tttactaacg tctggaaaga cgacaaaact ttagatcgtt
2581 acgctaacta tgagggctgt ctgtggaatg ctacaggcgt tgtggtttgt actggtgacg
2641 aaactcagtg ttacggtaca tgggttccta ttgggcttgc tatccctgaa aatgagggtg
2701 gtggctctga gggtggcggt tctgagggtg gcggttctga gggtggcggt actaaacctc
2761 ctgagtacgg tgatacacct attccgggct atacttatat caaccctctc gacggcactt
2821 atccgcctgg tactgagcaa aaccccgcta atcctaatcc ttctcttgag gagtctcagc
2881 ctcttaatac tttcatgttt cagaataata ggttccgaaa taggcagggt gcattaactg
2941 tttatacggg cactgttact caaggcactg accccgttaa aacttattac cagtacactc
3001 ctgtatcatc aaaagccatg tatgacgctt actggaacgg taaattcaga gactgcgctt
3061 tccattctgg ctttaatgag gatccattcg tttgtgaata tcaaggccaa tcgtctgacc
3121 tgcctcaacc tcctgtcaat gctggcggcg gctctggtgg tggttctggt ggcggctctg
3181 agggtggcgg ctctgagggt ggcggttctg agggtggcgg ctctgagggt ggcggttccg
3241 gtggcggctc cggttccggt gattttgatt atgaaaaaat ggcaaacgct aataaggggg
3301 ctatgaccga aaatgccgat gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt
3361 ctgtcgctac tgattacggt gctgctatcg atggtttcat tggtgacgtt tccggccttg
3421 ctaatggtaa tggtgctact ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg
3481 gtgacggtga taattcacct ttaatgaata atttccgtca atatttacct tctttgcctc
3541 agtcggttga atgtcgccct tatgtctttg gcgctggtaa accatatgaa ttttctattg
3601 attgtgacaa aataaactta ttccgtggtg tctttgcgtt tcttttatat gttgccacct
3661 ttatgtatgt attttcgacg tttgctaaca tactgcgtaa taaggagtct taataagaat
3721 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
3781 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
3841 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
3901 cttacgcatc tgtgcggtat ttcacaccgc atataaattg taaacgttaa tattttgtta
3961 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
4021 aaaatccctt ataaatcaaa agaatagccc gagatagggt tgagtgttgt tccagtttgg
4081 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
4141 cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc
4201 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
4261 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
4321 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
4381 cagggcgcgt actatggttg ctttgacggg tgcagtctca gtacaatctg ctctgatgcc
4441 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
4501 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
4561 aggttttcac cgtcatcacc gaaacgcgcg a
//
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pCantab5E载体应用举例
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