CIP106682

<< DSMZ << J.-H. Klemme, R1

TRYPTIC SOY BROTH OR TRYPTIC SOY AGAR (DIFCO 0369)

Tryptone 15.0 g

Soytone 5.0 g

NaCl 5.0 g

Agar 15.0 g

Distilled water 1.0 L

Adjust pH to 7.3.

996

RHODOSPIRILLACEAE MEDIUM (reduce yeast extract and pH)

(NH4)-acetate 0.50 g

CaCl2 · 2 H2O 0.05 g

Distilled water 1000 mL

Fe(III) citrate solution (0.1% in H2O) 5.00 mL

KH2PO4 0.50 g

L-Cysteiniumchloride 0.30 g

MgSO4 · 7 H2O 0.40 g

Na2-succinate 1.00 g

NaCl 0.40 g

NH4Cl 0.40 g

Resazurin (0.1%) 0.50 mL

Trace element solution SL-6 (see below) 1.00 mL

Vitamin B12 solution (10 mg in 100 mL H2O) 0.40 mL

Yeast extract 0.20 g

Reduce Medium 903 the yeast extract concentration to 0.2 g/L. Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 mL in 15 mL tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp. Adjust pH to 5.7.

Trace element solution SL-6

CoCl2· 6 H2O 0.20 g

CuCl2 ·2 H2O 0.01 g

Distilled water 1000 mL

H3BO3 0.30 g

MnCl2·4 H2O 0.03 g

Na2MoO4·2 H2O 0.03 g

NiCl2· 6 H2O 0.02 g

ZnSO4·7 H2O 0.10 g

B4513 

Hougardy, A., B. J. Tindall, and J. H. Klemme. 2000. Rhodopseudomonas rhenobacensis sp. nov., a new nitrate-reducing purple non-sulfur bacterium. Int.J.Syst.Evol.Microbiol. 50 : 985-992.