JCM16185

Desulfosporosinus Acidophilus Medium

Ca(NO3)2 x 5 H2O 0.02 g

D-Fructose 3.50 g

Distilled water 1000.00 mL

KCl 0.05 g

KH2PO4 10.00 g

L-Cysteine-HCl x H2O 0.50 g

MgSO4 x 7 H2O 0.50 g

(NH4)2SO4 0.45 g

Na2HPO4 1.00 g

Selenite-tungstate solution (see below) 1.00 mL

Trace element solution SL-10 (see below) 1.00 mL

Vitamin solution (see below) 10.00 mL

Yeast extract 0.50 g

Na-resazurin solution (0.1% w/v) 0.50 mL

Dissolve ingredients (except phosphates, vitamins, fructose and cysteine) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prepare a phosphate buffer stock solution with KH2PO4 (25% w/v) and Na2HPO4, adjust pH of the buffer to 5.8. Prior to inoculation add phosphate buffer, vitamins, fructose and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins and D-fructose should be sterilized by filtration. The pH of the complete medium should be at 5.8.

Selenite-tungstate solution

Distilled water 1000.0 mL

Na2SeO3 x 5 H2O 3.0 mg

NaOH 0.5 g

Na2WO4 x 2 H2O 4.0 mg

 

Vitamin solution (should be stored at -20°C)

Distilled water 1.0 L

p-Aminobenzonic acid 5.0 mg

Pyridoxin hydrochloride 10.0 mg

Thiamine-HCl x 2 H2O 5.0 mg

Vitamin B12 0.1 mg

Thioctic acid 5.0 mg

Riboflavin 5.0 mg

Pantothenic acid 5.0 mg

Nicotinic acid 5.0 mg

Folic acid 2.0 mg

Biotin 2.0 mg

 

Trace element solution SL-10

CoCl2·6H2O 190.0 mg

NiCl2·6H2O 24.0 mg

ZnCl2 70.0 mg

H3BO3 6.0 mg

Na2MoO4·2H2O 36.0 mg

MnCl2·4H2O 100.0 mg

HCl (25%; 7.7M) 10.0 mL

FeCl2·4H2O 1.5 g

Distilled water 990.0 mL

CuCl2·2H2O 2.0 mg

Trace element solution SL-10: First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 mL (This medium is modified from DSM #120: METHANOSARCINA MEDIUM)

B2469 

Alazard, D., M. Joseph, F. Battaglia-Brunet, J. L. Cayol, and B. Ollivier. 2010. Desulfosporosinus acidiphilus sp. nov.: a moderately acidophilic sulfate-reducing bacterium isolated from acid mining drainage sediments : New taxa: Firmicutes (Class Clostridia, Order Clostridiales, Family Peptococcaceae). Extremophiles. 14: 305-312.