JCM21509

AZOTOBACTER MEDIUM

CaCl2 x 2 H2O 0.10 g

MgSO4 x 7 H2O 0.10 g

Na2MoO4 x 2 H2O 5.00 mg

K2HPO4 0.90 g

KH2PO4 0.10 g

FeSO4 x 7 H2O 0.01 g

CaCO3 5.00 g

Agar 15.00 g

Distilled water 950.00 ml

Glucose 5.00 g

Mannitol 5.00 g

Adjust pH to 7.3. Sterilize glucose and mannitol separately (in 50 ml H2O) and add to the medium after autoclaving. Calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies.

B1418 

1981. Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 6. Int. J. Syst. Bacteriol. 31: 215-218. 

B2120 

Malik, K. A. 1988. A new freeze-drying method for the preservation of nitrogen-fixing and other fragile bacteria. J. Microbiol. Methods 8:259-271. 

B2411 

Thompson, J. P., Skerman, V. B. D. 1979. Azotobacteraceae - the taxonomy and ecology of the aerobic nitrogen-fixing bacteria.Academic Press. 

B2412 

Kirakosyan, A. V., Melkonyan, Z. 1964. New Azotobacter agile varieties from the soil of ARMSSR Dokl.Akad.Nauk Arm.SSR 17: 33-42. 

B2413 

De Smedt, J., Bauwens, M., Tytgat, R., de Ley, J. 1980. Intra- and intergeneric similarities of ribosomal ribonucleic acid cistrons of free living, nitrogen-fixing bacteria Int.J.Syst.Bacteriol. 30: 106-122. 

B2414 

Steinbuchel, A., Malik, K. A., Jendrossek, D. 1992. PHB and other polyhydroxyalkanoic acids as a basis for biodegradable plastics. In Proceedings of the 2nd Asia-Pacific Biotechnology Congress, Los Banos, Philippines, 1991 , pp. 141-153. Edited by L. L. Ilag and A. K. Raymundo, Kluwer Academic Publishers.