| 保藏机构: | JCM |
|---|---|
| 菌种名称: | JCM7849, JCM 7849 |
| 英文名称: | 产孢梭菌 |
| 中文名称 | Clostridium sporogenes |
| 存储人: | (Metchnikoff) Bergey et al. |
| 来源历史: | << IAM << S. Sugama << Inst. Pasteur, strain GO 1 |
| 其他保藏中心编号: | IAM 19234; JCM 7849 |
| 分离来源: | 土壤 |
| 菌种特性 | 厌氧菌 |
| 备注： | |
| 培养条件： | 37°C |
| 生物安全等级: | 2 |
| 氧气需求: | 需氧 |
| 培养基: |
TSB培养基：BEEF LIVER MEDIUM for Anaerobes
Tap water 1.0 L
Peptone 10.0 g
K2HPO4 1.0 g
Beef liver (cut in small pieces) 500.0 g
Soak beef liver with 1 L tap water overnight in refrigerator. Skim fat off top. Sterilize at 121°C for 10 minutes. Filter through cheesecloth. Save meat. Add peptone and K2HPO4. Adjust pH to 8.0. Filter through filter paper and make up volume to 1.0 L with tap water. Boil broth and cool under 100% N2.Place a small amount of CaCO3 in each tube, add meat to a depth of 1/2 inch, cover with broth to a total depth of 2 inches.
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| 模式菌株: | 否 |
| 参考文献: |
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