| 保藏机构: | KCTC |
|---|---|
| 菌种名称: | KCTC15143, KCTC15143 |
| 英文名称: | Rhodopseudomonas pentothenatexigens，五齿红假单胞菌 |
| 菌种又名: | |
| 中文名称: | 五齿红假单胞菌 |
| 菌种代号: | KCTC 15143; NBRC 108862; JA575 |
| 分离: | 土壤 |
| 来源历史: | << KCTC |
| 培养基: |
584
PYM Medium
Polypepton* 10 g
Distilled water 1 L
Agar (if needed) 15 g
Yeast extract 2 g
MgSO4·7H2O 1 g
pH 7.0 (*Prepared medium is available from Wako Pure Chemical Ind., Ltd., Osaka, Japan.)
924
Rhodopseudomonas medium
NaCl 0.4 g
NH4Cl 0.6 g
CaCl2·2H2O 0.05 g
Sodium pyruvate 3.0 g
Yeast extract 0.3 g
Ferric Citrate (0.1 % w/v) 5.0 mL
Micronutrient solution SL-7 1.0 mL
Vitamin B12 1.0 mL
KH2PO4 0.5 g
MgSO4·7H2O 0.2 g
final pH 7.0, add distilled water to 1.0 L.
Ferric Citrate (0.1 % w/v)
Ferric Citrate 0.1 g
ddH2O 100 mL
Ferric citrate was taken and add distilled water and dissolved by heating.
Micronutrient solution SL-7
ZnCl2 70 mg
MnCl2·4H2O 100 mg
CoCl2·6H2O 200 mg
H3BO3 60 mg
Na2MoO4·2H2O 40 mg
CuCl2·2H2O 20 mg
NiCl2·6H2O 20 mg
pH was adjusted to 6.8-7.0 with 1N NaOH/1N HCl before autoclaving.
|
| 培养条件: | 25°C 5天,厌氧 |
| 菌种说明: | 1.菌株復活：凍乾管破管後以0.2-0.5 ml無菌水回溶乾燥菌塊。回溶菌液必須全部吸取至 10 ml 的新鮮培養基中進行活化培養。 2.特殊培養條件：厭氧和光照。 3.增殖培養的接種量需求：接種菌液的體積必須佔新鮮培養基的 20% 至 50%。 |
| 生物安全等级: | 1 |
| 参考文献: |
B2333
Kumar, B. V., E.V.V. Ramaprasad, C. Sasikala, and C. V. Ramana 2013. Rhodopseudomonas pentothenatexigens sp. nov. and Rhodopseudomonas thermotolerans sp. nov., isolated from paddy soils. Int. J. Syst. Evol. Microbiol. 63: 200-207.
|
买家导航
