| 保藏机构: | KCTC |
|---|---|
| 菌种名称: | KCTC5963, KCTC5963 |
| 英文名称: | Rhodovulum phaeolacus，小红卵菌 |
| 菌种又名: | |
| 中文名称: | 小红卵菌 |
| 菌种代号: | JA580; KCTC 5963; NBRC 107649 |
| 分离: | 棕色池塘的沉积物样本 |
| 来源历史: | << KCTC |
| 培养基: |
926
Rhodovulum medium
casein enzyme hydrolysate type I 1.0 g
CaCl2·2H2O 0.15 g
Ferric Citrate (0.1 % w/v) (refer to Medium 924, see below) 5.0 mL
KH2PO4 0.5 g
MgSO4·7H2O 2.0 g
Micronutrient solution SL7 (refer to Medium 924, see below) 1.0 mL
NaCl 20.0 g
NaS·9H2O (1M) 1.0 mL
NH4Cl 0.6 g
Sodium glutamate 1.0 g
Sodium pyruvate 1.0 g
Vitamin B12 (refer to Medium 924, see below) 1.0 mL
Yeast extract 0.3 g
add distilled water to 1.0 L, final pH was adjusted to 6.8-7.0 with 1N NaOH/1N HCl before autoclaving.
Ferric Citrate (0.1 % w/v)
ddH2O 100 mL
Ferric Citrate 0.1 g
Ferric citrate was taken and add distilled water and dissolved by heating.
Micronutrient solution SL-7
CoCl2·6H2O 200 mg
MnCl2·4H2O 100 mg
NiCl2·6H2O 20 mg
ZnCl2 70 mg
Na2MoO4·2H2O 40 mg
H3BO3 60 mg
CuCl2·2H2O 20 mg
Vitamin B12 (2 mg) was dissolved in 100 mL distilled water and filter sterilized by using Millipore cellulose acetate membrane filter (0.2 mm) into a sterile screw cap tube.
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| 培养条件: | 30°C 5天,厌氧 |
| 菌种说明: | 1.冷凍保存法：抗凍劑為 5% DMSO (最終濃度)。 2.冷凍小管只能解凍一次並且完全活化，不可重複冷凍解凍。 3.菌株復活：冷凍小管立即置於37-40℃水浴中，不時搖動，使冷凍菌液完全融解。回溶的菌液必須全部吸取至 10 ml 的新鮮培養基中進行活化培養。 4.特殊培養條件：厭氧和光照。 5.增殖培養的接種量需求：接種菌液的體積必須佔新鮮培養基的 20% 至 50%。 |
| 生物安全等级: | 1 |
| 参考文献: |
B2336
Lakshmi, K.V.N.S., C. Sasilaka, V. V. Ramana, E. V. V. Ramaprasad, and C. V. Ramana 2011. Rhodovulum phaeolacus sp. nov. a phototrophic alphaproteobacterium isolated from a brown pond. J. Gen. Appl. Microbiol. 57: 145-151.
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