| 保藏机构: | NBRCC |
|---|---|
| 菌种名称: | NBRC102145 , NBRC 102145 |
| 英文名称: | Thiomonas delicata |
| 中文名称: | 得利卡硫单胞菌 |
| 菌种又名: | Thiobacillus delicatus |
| 存储日期: | 2006/10/26 |
| 历史来源: | DSM 5495 <- K.O. Stetter & H. Huber, Hö5 |
| 其他保藏中心编号: | DSM 5495 |
| 菌种代号: | Hö5 |
| 培养基: |
958
Thiobacillus Cuprinus medium
MgSO4 x7 H2O 3.45 g
NH4Cl 1.25 g
CaCl x 2H2O 0.14 g
K2HPO4 0.14 g
KH2PO4 0.14 g
NaCl 0.5 g
Trace element sloution 10 mL
NiCl2 x 6 H2O 2 mg
Distilled water 1000 mL
KCl 0.33 g
MgCl2 x 6 H2O 2.75 g
Adjust pH to 3.5 with sulfuric acid. After autoclaving add sterile sulfur powder (0.05% w slash v), or sulfitic ore (2% w slash v), or yeast extract (0.05% w slash v).
Trace element solution
Nitrilotriacetic acid 1.5 g
MgSO4 x 7 H2O 3 g
MnSO4 x H2O 0.5 g
NaCl 1 g
FeSO4 x 7 H2O 0.1 g
CoSO4 x 7 H2O 0.18 g
CaCl2 x 2 H2O 0.1 g
ZnSO4 x 7 H2O 0.18 g
CuSO4 x 5 H2O 0.01 g
KAl(SO4)2 x 12 H2O 0.02 g
H3BO3 0.01 g
Na2MoO4 x 2 H2O 0.01 g
NiCl2 x 6 H2O 0.03 g
Na2SeO3 x 5 H2O 0.3 mg
Na2WO4 x 2 H2O 0.4 mg
Distilled water 1000 mL
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).
|
| 培养温度: | 30°C |
| 是否需氧: | |
| 分离来源: | |
| 生物安全等级: | 1 |
| 菌株类型: | 是 |
| 参考文献: |
B2097
Huber, H., Stetter, K. O. 1990. Thiobacillus cuprinus sp. nov., a novel facultatively organotrophic metal-mobilizing bacterium. Appl.Environ.Microbiol. 56: 315-322.
B2098
Moreira, D., Amils, R. 1997. Phylogeny of Thiobacillus cuprinus and other mixotrophic Thiobacilli: proposal for Thiomonas gen. nov. Int.J.Syst.Bacteriol. 47: 522-528.
B2099
Battaglia-Brunet, F., El Achbouni, H., Quemeneur, M., Hallberg, K. B., Kelly, D. P., Joulian, C. 2011. Proposal that the arsenite-oxidizing organisms Thiomonas cuprina and 'Thiomonas arsenivorans' be reclassified as strains of Thiomonas delicata, and emended description of Thiomonas delicata. Int. J. Syst. Evol. Microbiol. 61: 2816-2821.
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