| 保藏机构: | NBRC |
|---|---|
| 菌种名称: | NBRC15271 , NBRC 15271 |
| 英文名称: | Magnetospirillum gryphiswaldense |
| 中文名称: | 磁螺菌 |
| 菌种又名: | Magnetospirillum gryphiswaldense Schleifer et al. 1992 |
| 存储日期: | 1992/03/02 |
| 历史来源: | IFO 15271 <- DSM 6361 <- D. Schuler, MSR-1 |
| 其他保藏中心编号: | DSM 6361=IAM 14722=JCM 21280 |
| 菌种代号: | MSR-1 |
| 培养基: |
Magntospirillum medium
Yeast extract 0.1g
Na-thioglycolate 0.5g
Distilled water 1000ml
K2HPO4 0.5g
Fe (Ⅲ) citrate 20μM
Na-acetate 1g
NH4Cl 0.1g
Adjust pH to 6.8. Prepare medium 2-3 days before use. Dispense 5 ml of medium in sterile screw capped culture tubes. Inoculate with 10% (v/v) culture. Incubate tubes upright, do not shake.
611
Magntospirillum medium
KH2PO4 0.68g
Na-thioglycolate 0.05g
L(+)-Tartaric acid 0.37g
Succinic acid 0.37g
Trace elements solution 5ml
Resazurin 0.5mg
Distilled water 1000ml
NaNO3 0.12g
Na-acetate 0.05g
Vitamin solution 10ml
Fe(Ⅲ) quinate solution 2ml
Dissolve components in the order given. Adjust pH to 6.75 with NaOH and boil medium for 1 min. Purge medium with N2 gas for 10 min. Under the same atmosphere, anaerobically fill tubes to 1/3 of their volume and seal. Autoclave at 121℃ for 15 min. Before inoculation, ad
Trace elements solution
Nitrilotriacetic acid 1.5g
MgSO4·7 H2O 3g
MnSO4·2 H2O 0.5g
NaCl 1g
CaCl2·2 H2O 0.1g
Zn SO4·7 H2O 0.18g
Cu SO4·5 H2O 0.01g
Kal(SO4)2·12 H2O 0.02g
H3BO3 0.01g
Na2MoO4·2 H2O 0.01g
Distilled water 1000ml
FeSO4·7 H2O 0.1g
CoSO4·7 H2O 0.18g
NiCl2·6 H2O 0.025g
Na2SeO3·5 H2O 0.3mg
Na2WO4·2 H2O 0.4 mg
Trace elements solution:First dissolve nitrilotriacetic acid adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).
Vitamin solution
Biotin 2mg
Folic acid 2mg
Pyridoxine-HCl 10mg
Thiamine-HCl·2 H2O 5mg
Riboflavin 5mg
Vitamin B12 0.1mg
p-Aminobenzoic acid 5mg
Lipoic acid 5mg
Distilled water 1000ml
Nicotinic acid 5mg
D-Ca-pantothenate 5mg
Ferric quinate solution, 0.01 M
FeCl3·6H2O 0.45g
Quinic acid 0.19g
Distilled water 100ml
Ferric quinate solution:Dissolve and autoclave at 121℃ for 15 min.
|
| 培养温度: | 30°C |
| 是否需氧: | |
| 分离来源: | |
| 生物安全等级: | 1 |
| 菌株类型: | 是 |
| 参考文献: | Schleifer, K. H., D. Schuler, S. Spring, M. Weizenegger, R. Amann, W. Ludwig, and M. Kohler. 1991. The genus Magnetospirillum gen. nov. description of Magnetospirillum gryphiswaldense sp. nov. and transfer of Aquaspirillum magnetotacticum to Magnetospirillum magnetotacticum comb. nov. Syst. Appl. Microbiol. 14 : 379–385. |
NBRC15271
价格：3200元
代购价格：询价
联系方式：I47-825O-882O
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