| 保藏机构: | NCIMB |
|---|---|
| 菌种名称: | NCIMB8252, NCIMB 8252 |
| 英文名称: | Rhodopseudomonas pseudopalustris，假性沼泽红假单胞菌 |
| 菌种又名: | Rhodopseudomonas rutila |
| 中文名称: | 假性沼泽红假单胞菌 |
| 菌种代号: | NBRC 100419; DSM 123; NCIMB 8252 |
| 分离: | 地表水或泥浆 |
| 来源历史: | << NBRC << NCIMB << S.R. Elsden << C.B. van Niel, ATH 2.1.6 |
| 培养基: |
584
PYM Medium
Polypepton* 10 g
Distilled water 1 L
Agar (if needed) 15 g
Yeast extract 2 g
MgSO4·7H2O 1 g
pH 7.0 (*Prepared medium is available from Wako Pure Chemical Ind., Ltd., Osaka, Japan.)
903
RHODOSPIRILLACEAE MEDIUM (modified)
Na2-succinate 1.00 g
(NH4)-acetate 0.50 g
Fe(III) citrate solution (0.1% in H2O) 5.00 mL
KH2PO4 0.50 g
MgSO4 · 7 H2O 0.40 g
NaCl 0.40 g
NH4Cl 0.40 g
CaCl2 · 2 H2O 0.05 g
Vitamin B12 solution (10 mg in 100 mL H2O) 0.40 mL
Trace element solution SL-6 (see below) 1.00 mL
Resazurin (0.1%) 0.50 mL
Distilled water 1000 mL
Yeast extract 0.30 g
L-Cysteiniumchloride 0.30 g
Adjust pH to 6.8. Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp.
Trace element solution SL-6
ZnSO4·7 H2O 0.10 g
MnCl2·4 H2O 0.03 g
H3BO3 0.30 g
CoCl2· 6 H2O 0.20 g
CuCl2 ·2 H2O 0.01 g
NiCl2· 6 H2O 0.02 g
Na2MoO4·2 H2O 0.03 g
Distilled water 1000 mL
|
| 培养条件: | 30°C 7天 |
| 菌种说明: |
1.菌株復活：冷凍管立即置於37℃恆溫水槽回溶，回溶菌液必須全部吸取至 10 mL 的新鮮培養基中進行活化培養。冷凍管菌液不要重複冷凍解凍。 2.特殊培養條件：厭氧和光照。 3.增殖培養的接種量需求：接種菌液的體積必須佔新鮮培養基的 20% 至 50%。 |
| 生物安全等级: | 1 |
| 参考文献: |
B2160
Van Niel, C.B. 1944 . The culture, general physiology, morphology and classification of the nonsulfur purple and brown bacteria. Bacteriol. Rev. 8: 1-118.
B2207
Pfennig, N. and H. G. Truper. 1971. Type and neotype strains of the species of phototrophic bacteria maintained in pure culture. Int. J. Syst. Bacteriol. 21 : 19–24.
B2334
Venkata Ramana V., S. Kalyana Chakravarthy, P. Shalem Raj, B. Vinay Kumar, E. Shobha, E. V. V. Ramaprasad, C. Sasikala, and C. V. Ramana 2012. Descriptions of Rhodopseudomonas parapalustris sp. nov., Rhodopseudomonas harwoodiae sp. nov. and Rhodopseudomonas pseudopalustris sp. nov., and emended description of Rhodopseudomonas palustris. Int. J. Syst. Evol. Microbiol. 62 : 1790-1798.
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买家导航
NCIMB8252标准菌株基本信息
常用微生物培养基推荐（可定制）
NCIMB8252标准菌株说明书pdf版和相关资料下载
NCIMB8252标准菌株应用举例
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