| 质粒名称: | pT7-VP7SA11 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 89167 |
| 存储实验室: | Takeshi Kobayashi |
|
载体骨架基本信息 |
|
| 载体名称: | p3E5 |
| 载体出品公司: | -- |
| 空载体大小: | 3074 bp |
| 完整质粒大小: | 4137 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Rotavirus SA11 VP7 |
| 物种来源: | Rotavirus SA11 |
| 插入大小: | 1063 bp |
| 基因突变: | -- |
| GenBank编号 | LC178569 |
| 启动子: | T7 promotor |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | T7 forward CTGTGGATAACCGTATTACCG |
| 3’测序引物 | T7 terminal GCTAGTTATTGCTCAGCGG |
|
文献引用方法 |
|
| 材料和方法部分: | pT7-VP7SA11 was a gift from Takeshi Kobayashi (Addgene plasmid # 89167 ; http://n2t.net/addgene:89167 ; RRID:Addgene_89167) |
| 参考文献部分: | Entirely plasmid-based reverse genetics system for rotaviruses. Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M, Matsuura Y, Taniguchi K, Kobayashi T. Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 10.1073/pnas.1618424114. 10.1073/pnas.1618424114 PubMed 28137864 |
买家导航
pT7VP7SA11载体基本信息
pT7VP7SA11载体图谱质粒图谱和多克隆位点信息
pT7VP7SA11载体简介
pT7VP7SA11载体序列
LOCUS pT7-VP7SA11 4136 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Express Rotavirus SA11 VP7 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pT7-VP7SA11
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4136)
AUTHORS Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M,
Matsuura Y, Taniguchi K, Kobayashi T
TITLE Entirely plasmid-based reverse genetics system for rotaviruses.
JOURNAL Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi:
10.1073/pnas.1618424114.
PUBMED 28137864
REFERENCE 2 (bases 1 to 4136)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4136
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(40..62)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 162..181
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 391..410
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
promoter 700..718
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 700..717
/label=SP6
/note="SP6 promoter, forward primer"
terminator 786..833
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind 829..847
/label=T7 Term
/note="T7 terminator, reverse primer"
promoter complement(2055..2073)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2157..2174)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2328..2916)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2408..2427)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3087..3947)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3710..3729
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3948..4052)
/gene="bla"
/label=AmpR promoter
primer_bind 4120..2
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
61 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
121 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
181 actgagagtg caccattcga cgctctccct tatgcgactc ctgcattaag aagcagccca
241 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcaagg agatggcgcc
301 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
361 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
421 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctggctagc
481 gatgaccctg ctgattggtt cgctgaccat ttccgggtgc gggacggcgt taccagaaac
541 tcagaaggtt cgtccaacca aaccgactct gacggcagtt tacgagagag atgatagggt
601 ctgcttcagt aagccagatg ctacacaatt aggcttgtac atattgtcgt tagaacgcgg
661 ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac actatagaat
721 acaagcttgc atgcctgcag gtcgactcta gaggatctcg atccggatat agttcctcct
781 ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc tagttattgc
841 tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc cggatccttt
901 tttttgagct ctcccttagc catccgagtg gacgacgtcc tccttcggat gcccaggtcg
961 gaccgcgagg aggtggagat gccatgccga cccggtcaca tcatacaatt ctaacctaag
1021 ttatacctac actctgtaat aaaatgctgc tgaatttagt gatcttgatc ttttggacat
1081 aacttgtatt atctgatcta catagtctac tacagtataa aaaacttgcc accatttttt
1141 ccagttaatt cgcatcatcc gttctgtctg tggtgcagta gttggatcag cagttatatc
1201 gaggatgtca gaaccaccaa cttgtataac ggctacgttt tctcttggtc ccaatttctt
1261 acagtttcta atagtacacg ttgctgttgt gacatccagc ttatgattaa cgccatcaac
1321 cacgtcagta attaccaact tttcagctgt cgcaacttct tcaaaagttg tagcatcagt
1381 tgtcaagcat ccaattccaa gagtttgtgt attaagtgga catactttaa ttgtacatga
1441 tgagcccatt gatatccatt tattcgcttc gtcagtttgc tgataataat acagagtaat
1501 atccattgga ttacacaacc attcgtttaa tattagatcc gcaagttctg acatatccaa
1561 ttgcaacgtc gcgtcatatt tcattagtac tacgttataa tcacaataca actgcggatc
1621 aacagaaaac gatgcaatgt tagtatattc tttaaaatat acggatccag ttggccaccc
1681 tttcgtaaga aatagttgtg acagtgtgtc tttccatgaa ttatcgttta tttcagtcgc
1741 agcctcagtc ggataatata ggcaaagtgt agaagtgagg aatggttctt cttgcgttga
1801 attagcgtat gcaatgtcca tggagcctgt gattggaaga ttaataccat aattttgtgc
1861 tctgagaaat ggtgacaata tcacaattat aaaaagaaat ctataaatta taaagtccat
1921 tattctagtt aatgatttaa gtatgtaatt tagtagaata atcgatatca gaaaggttag
1981 aactgtggta tattcaatac catacattaa aaggagctaa ccgctagcca aacggaaatt
2041 ctctcttttt aaagcctata gtgagtcgta ttaaccggga tcgatatccc cctgcattaa
2101 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
2161 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
2221 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
2281 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
2341 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
2401 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
2461 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
2521 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
2581 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
2641 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
2701 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
2761 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
2821 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
2881 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
2941 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
3001 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
3061 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
3121 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
3181 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
3241 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
3301 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
3361 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
3421 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
3481 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
3541 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
3601 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
3661 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
3721 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
3781 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
3841 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
3901 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
3961 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
4021 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
4081 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgag
//
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