| 质粒名称: | pSQT1313 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 53370 |
| 存储实验室: | Keith Joung |
|
载体骨架基本信息 |
|
| 载体名称: | MLM3636 |
| 载体出品公司: | Joung lab |
| 载体抗性: | 氨苄霉素 |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | None |
| 启动子 | U6 |
| 克隆方法: | 未知 |
| 5' 克隆位点: | |
| 3' 克隆位点: | |
| 5' 测序引物 | OS280 (5’-CAGGGTTATTGTCTCATGAGCGG-3’) |
| 3' 测序引物 | |
|
文献引用方法 |
|
| 材料和方法部分: | pSQT1313 was a gift from Keith Joung (Addgene plasmid # 53370) |
| 参考文献部分: | Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908. 10.1038/nbt.2908 PubMed 24770325 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSQT1313 2320 bp DNA SYN
DEFINITION pSQT1313
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..2320
/organism="pSQT1313"
/mol_type="other DNA"
promoter 67..323
/label="hU6_promoter"
misc_feature 253..272
/label="LKO1_5_primer"
gene complement(1330..2190)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(1330..2190)
/label="ORF frame 2"
promoter complement(2232..2260)
/label="AmpR_promoter"
ORIGIN
1 GACGTCGCTA GCTGTACAAA AAAGCAGGCT TTAAAGGAAC CAATTCAGTC GACTGGATCC
61 GGTACCAAGG TCGGGCAGGA AGAGGGCCTA TTTCCCATGA TTCCTTCATA TTTGCATATA
121 CGATACAAGG CTGTTAGAGA GATAATTAGA ATTAATTTGA CTGTAAACAC AAAGATATTA
181 GTACAAAATA CGTGACGTAG AAAGTAATAA TTTCTTGGGT AGTTTGCAGT TTTAAAATTA
241 TGTTTTAAAA TGGACTATCA TATGCTTACC GTAACTTGAA AGTATTTCGA TTTCTTGGCT
301 TTATATATCT TGTGGAAAGG ACGAAACACC GTTCACTGCC GTATAGGCAG TGAGACGATT
361 AATGCGTCTC AGTTTTAGAG CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA
421 CTTGAAAAAG TGGCACCGAG TCGGTGCGTT CACTGCCGTA TAGGCAGTTT TTTTAAGCTT
481 GGGCCGCTCG AGGTACCTCT CTACATATGA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG
541 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA
601 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
661 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
721 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
781 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
841 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
901 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
961 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
1021 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
1081 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
1141 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG
1201 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA
1261 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG
1321 GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG
1381 TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC
1441 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC
1501 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC
1561 CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG
1621 TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT
1681 GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG
1741 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT
1801 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG
1861 ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG
1921 ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT
1981 AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT
2041 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC
2101 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT
2161 AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT
2221 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA
2281 AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT
//
