| 质粒名称: | pAAV-FLEX-ArchT-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 28307 |
| 存储实验室: | Edward Boyden |
|
载体骨架基本信息 |
|
| 载体名称: | AAV-FLEX |
| 载体出品公司: | Scott Sternson |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5769 bp |
| 完整质粒大小: | 6513 bp |
| 载体类型: | 哺乳细胞表达，AAV, Cre/Lox ; Adeno-associated virus |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ArchT-GFP |
| 物种来源: | H. strain TP009 |
| 插入大小: | 744 bp |
| 基因又名: | archaerhodopsin;archaerhodopsin TP009;ArchT |
| 基因突变: | N/A |
| 启动子 | CAG |
| 标签/融合蛋白: |
GFP (C terminal on insert)
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | gctctagagcctctgctaacc |
| 3' 测序引物 | gcagcgtatccacatagcg |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV-FLEX-ArchT-GFP was a gift from Edward Boyden (爱迪基因 plasmid # 28307) |
| 参考文献部分: | A high-light sensitivity optical neural silencer: development and application to optogenetic control of non-human primate cortex. Han X, Chow BY, Zhou H, Klapoetke NC, Chuong A, Rajimehr R, Yang A, Baratta MV, Winkle J, Desimone R, Boyden ES. Front Syst Neurosci. 2011;5:18. Epub 2011 Apr 13. 10.3389/fnsys.2011.00018 PubMed 21811444 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAAV-FLEX-ArchT-GFP 6513 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAAV-FLEX-ArchT-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6513)
AUTHORS Han X, Chow BY, Zhou H, Klapoetke NC, Chuong A, Rajimehr R, Yang A,
Baratta MV, Winkle J, Desimone R, Boyden ES
TITLE A high-light sensitivity optical neural silencer: development and
application to optogenetic control of non-human primate cortex.
JOURNAL Front Syst Neurosci. 2011;5:18. Epub 2011 Apr 13.
PUBMED 21811444
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pAAV-FLEX-ArchT-GFP|
FEATURES Location/Qualifiers
source 1..6513
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(11..28)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(262..281)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1564..1583
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2069..2090)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2281..2300
/label=F1ori-R
/note="F1 origin, reverse primer"
repeat_region 2459..2588
/label=AAV2 ITR
promoter 2901..3178
/label=chicken beta-actin promoter
primer_bind 3458..3480
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 3542..3561
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
protein_bind complement(3596..3629)
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites."
protein_bind complement(3692..3725)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS complement(3738..4454)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(3783..3804)
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind 4132..4151
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4391..4412
/label=EGFP-N
/note="EGFP, reverse primer"
regulatory 5210..5219
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
protein_bind 5236..5269
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites."
protein_bind 5340..5373
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 5415..6003
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5468..5488)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5886..5897)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 6046..6167
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6057..6076)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 6111..6130
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
repeat_region 6329..6458
/label=AAV2 ITR
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
2701 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
2761 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
2821 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
2881 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
2941 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
3001 gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
3061 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
3121 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
3181 agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
3241 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
3301 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
3361 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg
3421 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
3481 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
3541 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgga tccccataac
3601 ttcgtataaa gtatcctata cgaagttata tcaaaatagg aagaccaatg cttcaccatc
3661 gacccgaatt gccaagcatc accatcgacc cataacttcg tataatgtat gctatacgaa
3721 gttatactag gatcccctta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
3781 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
3841 gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
3901 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
3961 aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg
4021 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
4081 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
4141 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
4201 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag
4261 gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac
4321 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
4381 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
4441 tcgcccttgc tcactgctac taccggtcgg ggctcggggg cctcggtgtc gcccaggatg
4501 gccctgctcc tcagcaggat gaagccgaag cccactttgg cggtcacgtc cagcaccatg
4561 aacagcaggg tctcgattcc cagtcccacc actccagctc cctcggttcc gatgatccac
4621 aggatggggt aggcggtcca cagcaccagc accagggcgg tcagggtgtt gaaggtgcta
4681 gccacctctg gtcccctctc cttagcagca gccctcaggc tggtagccag gaagtacagc
4741 accacgatca tgcagatggt gctgaacagc caccagctgt acctagccag tggggtgtgg
4801 ctcagggcgc cgatcaggcc ggtcacgatc atcagggcgt ccactcccac cagggttccg
4861 atgctcaccc tgtccacctt agccagcagg gccaggtcga gcagaagcaa aggggtggtg
4921 aacagccagt cggcgtatct ggcgtagtag atgtccagca cctcgccggc cacggtcacc
4981 tcggtcaggc cgatgccgaa gaacatgctc aggtaggcgg cgctggcgat gccgggcacc
5041 aggattgtga tgctgtagta ctccctggcc tccttgtcgg tcacgcccca gcccttcacg
5101 atgaagtaga aggtgccgat cagcatcagc agggttccga ttcccagcca cagggtctct
5161 ggccttccgt cgcccagcag gtcgtatcct gcctgcagag cgatggggtc catggtggcg
5221 gtaccgaatt ctagtataac ttcgtatagg atactttata cgaagttatc attgggattc
5281 ttcctatttt gatccaagca tcaccatcga ccctctagtc cagatctcac catcgaccca
5341 taacttcgta tagcatacat tatacgaagt tatgtccctc gaagaggttc gaattcgata
5401 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
5461 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
5521 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
5581 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
5641 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
5701 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
5761 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
5821 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
5881 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
5941 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
6001 cgcatcgata ccgtcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg
6061 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
6121 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
6181 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa
6241 acctctacaa atgtggtaaa atcgataagg atcttcctag agcatggcta cgtagataag
6301 tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc
6361 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
6421 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcattaatga atcggccaac
6481 gcgcggggag aggcggtttg cgtattgggc gct
//
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