| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pYES2-NTC, pYES2/NT C |
| 质粒类型: | 酿酒酵母蛋白表达载体 |
| 表达水平: | 高拷贝 |
| 诱导方法: | 半乳糖 |
| 启动子: | GAL1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6037 bp |
| 5' 测序引物及序列: | T7: TAATACGACTCACTATAGGG |
| 3' 测序引物及序列: | CYC1 Terminator: GTGACATAACTAATTACATGATG |
| 载体标签: | N-His, N-Xpress, C-His, C-V5 |
| 载体抗性: | 氨苄 |
| 筛选标记: | URA3 |
| 备注: | 利用半乳糖诱导蛋白在酿酒酵母中表达。 |
| 产品目录号: | V8252-20 |
| 稳定性: | 稳定 Stable |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pYES2-NTC载体质粒基本信息
pYES2-NTC质粒图谱载体图谱和pYES2-NTC载体序列质粒序列多克隆位点信息
pYES2-NTC质粒载体简介
pYES2 NTC的是一个6.0 kb的载体，设计用来在酿酒酵母（Saccharomyces cerevisiae）中诱导表达重组蛋白。
该载体包含以下元素：
1. 酵母GAL1启动子，能够在酿酒酵母中被半乳糖高水平的诱导蛋白表达目的蛋白，同时能够被葡萄糖抑制表达
2.多克隆位点可以使用的很多限制酶切位点，便于基因插入。
3.CYC1终止子能够有效终止mRNA的转录。
4.能够利用URA3基因筛选带有ura3基因型的酵母宿主菌株转化子。
5.氨苄抗性基因能够方便在大肠杆菌中的进行载体筛选。
6. 载体本身带有N端His和Xpress标签，同时载体C端也带有V5抗原表位和His纯化标签。
8. 在酵母中，利用载体上的2μ复制子维持质粒的高拷贝。
pYES2-NTC质粒序列载体序列
LOCUS pYES2/NT C 6037 bp DNA circular SYN
DEFINITION pYES2/NT C
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..6037
/organism="pYES2/NT C"
/mol_type="other DNA"
promoter 1..451
/label="GAL1_promoter"
misc_feature 414..437
/label="GAL1_primer"
promoter 475..493
/label="T7_promoter"
misc_feature 542..560
/label="Xpress_fwd_primer"
misc_feature 543..575
/label="T7_leader"
misc_feature 579..602
/label="Xpress_EK"
terminator 790..1035
/label="CYC1_terminator"
misc_feature complement(799..817)
/label="CYC1_primer"
misc_feature complement(799..817)
/label="pYESTrp_rev_primer"
rep_origin complement(1263..1882)
/label="pBR322_origin"
CDS complement(2037..2897)
/label="ORF frame 3"
CDS complement(2993..3796)
/label="ORF frame 1"
gene complement(2996..3796)
/label="URA3"
/gene="URA3"
CDS 3285..3761
/label="ORF frame 3"
promoter complement(3798..4023)
/label="URA3_promoter"
rep_origin 4026..5497
/label="2micron_origin"
ORIGIN
1 ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT
61 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
121 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
181 CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
241 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
301 TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
361 GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC
421 CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC
481 GACTCACTAT AGGGAATATT AAGCTTACCA TGGGGGGTTC TCATCATCAT CATCATCATG
541 GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA
601 AGGTACCCGG ATCCAGTGTG GTGGAATTCT GCAGATATCC AGCACAGTGG CGGCCGCTCG
661 AGTCTAGAGG GCCCTTCGAA GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
721 CGCGTACCGG TCATCATCAC CATCACCATT GAGTTTAAAC CCGCTGATCC TAGAGGGCCG
781 CATCATGTAA TTAGTTATGT CACGCTTACA TTCACGCCCT CCCCCCACAT CCGCTCTAAC
841 CGAAAAGGAA GGAGTTAGAC AACCTGAAGT CTAGGTCCCT ATTTATTTTT TTATAGTTAT
901 GTTAGTATTA AGAACGTTAT TTATATTTCA AATTTTTCTT TTTTTTCTGT ACAGACGCGT
961 GTACGCATGT AACATTATAC TGAAAACCTT GCTTGAGAAG GTTTTGGGAC GCTCGAAGGC
1021 TTTAATTTGC AAGCTGCGGC CCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT
1081 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
1141 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
1201 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGCCCAGGAA CCGTAAAAAG
1261 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
1321 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
1381 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
1441 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
1501 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
1561 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
1621 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
1681 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT
1741 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
1801 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA
1861 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
1921 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
1981 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
2041 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
2101 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGC GCTTACCATC TGGCCCCAGT
2161 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
2221 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
2281 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
2341 GTTGGCATTG CTACAGGCAT CGTGGTGTCA CTCTCGTCGT TTGGTATGGC TTCATTCAGC
2401 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
2461 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
2521 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
2581 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
2641 TGCCCGGCGT CAATACGGGA TAATAGTGTA TCACATAGCA GAACTTTAAA AGTGCTCATC
2701 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
2761 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
2821 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
2881 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATGGGTAA TAACTGATAT AATTAAATTG
2941 AAGCTCTAAT TTGTGAGTTT AGTATACATG CATTTACTTA TAATACAGTT TTTTAGTTTT
3001 GCTGGCCGCA TCTTCTCAAA TATGCTTCCC AGCCTGCTTT TCTGTAACGT TCACCCTCTA
3061 CCTTAGCATC CCTTCCCTTT GCAAATAGTC CTCTTCCAAC AATAATAATG TCAGATCCTG
3121 TAGAGACCAC ATCATCCACG GTTCTATACT GTTGACCCAA TGCGTCTCCC TTGTCATCTA
3181 AACCCACACC GGGTGTCATA ATCAACCAAT CGTAACCTTC ATCTCTTCCA CCCATGTCTC
3241 TTTGAGCAAT AAAGCCGATA ACAAAATCTT TGTCGCTCTT CGCAATGTCA ACAGTACCCT
3301 TAGTATATTC TCCAGTAGAT AGGGAGCCCT TGCATGACAA TTCTGCTAAC ATCAAAAGGC
3361 CTCTAGGTTC CTTTGTTACT TCTTCTGCCG CCTGCTTCAA ACCGCTAACA ATACCTGGGC
3421 CCACCACACC GTGTGCATTC GTAATGTCTG CCCATTCTGC TATTCTGTAT ACACCCGCAG
3481 AGTACTGCAA TTTGACTGTA TTACCAATGT CAGCAAATTT TCTGTCTTCG AAGAGTAAAA
3541 AATTGTACTT GGCGGATAAT GCCTTTAGCG GCTTAACTGT GCCCTCCATG GAAAAATCAG
3601 TCAAGATATC CACATGTGTT TTTAGTAAAC AAATTTTGGG ACCTAATGCT TCAACTAACT
3661 CCAGTAATTC CTTGGTGGTA CGAACATCCA ATGAAGCACA CAAGTTTGTT TGCTTTTCGT
3721 GCATGATATT AAATAGCTTG GCAGCAACAG GACTAGGATG AGTAGCAGCA CGTTCCTTAT
3781 ATGTAGCTTT CGACATGATT TATCTTCGTT TCCTGCAGGT TTTTGTTCTG TGCAGTTGGG
3841 TTAAGAATAC TGGGCAATTT CATGTTTCTT CAACACTACA TATGCGTATA TATACCAATC
3901 TAAGTCTGTG CTCCTTCCTT CGTTCTTCCT TCTGTTCGGA GATTACCGAA TCAAAAAAAT
3961 TTCAAAGAAA CCGAAATCAA AAAAAAGAAT AAAAAAAAAA TGATGAATTG AATTGAAAAG
4021 CTAGCTTATC GATGATAAGC TGTCAAAGAT GAGAATTAAT TCCACGGACT ATAGACTATA
4081 CTAGATACTC CGTCTACTGT ACGATACACT TCCGCTCAGG TCCTTGTCCT TTAACGAGGC
4141 CTTACCACTC TTTTGTTACT CTATTGATCC AGCTCAGCAA AGGCAGTGTG ATCTAAGATT
4201 CTATCTTCGC GATGTAGTAA AACTAGCTAG ACCGAGAAAG AGACTAGAAA TGCAAAAGGC
4261 ACTTCTACAA TGGCTGCCAT CATTATTATC CGATGTGACG CTGCAGCTTC TCAATGATAT
4321 TCGAATACGC TTTGAGGAGA TACAGCCTAA TATCCGACAA ACTGTTTTAC AGATTTACGA
4381 TCGTACTTGT TACCCATCAT TGAATTTTGA ACATCCGAAC CTGGGAGTTT TCCCTGAAAC
4441 AGATAGTATA TTTGAACCTG TATAATAATA TATAGTCTAG CGCTTTACGG AAGACAATGT
4501 ATGTATTTCG GTTCCTGGAG AAACTATTGC ATCTATTGCA TAGGTAATCT TGCACGTCGC
4561 ATCCCCGGTT CATTTTCTGC GTTTCCATCT TGCACTTCAA TAGCATATCT TTGTTAACGA
4621 AGCATCTGTG CTTCATTTTG TAGAACAAAA ATGCAACGCG AGAGCGCTAA TTTTTCAAAC
4681 AAAGAATCTG AGCTGCATTT TTACAGAACA GAAATGCAAC GCGAAAGCGC TATTTTACCA
4741 ACGAAGAATC TGTGCTTCAT TTTTGTAAAA CAAAAATGCA ACGCGACGAG AGCGCTAATT
4801 TTTCAAACAA AGAATCTGAG CTGCATTTTT ACAGAACAGA AATGCAACGC GAGAGCGCTA
4861 TTTTACCAAC AAAGAATCTA TACTTCTTTT TTGTTCTACA AAAATGCATC CCGAGAGCGC
4921 TATTTTTCTA ACAAAGCATC TTAGATTACT TTTTTTCTCC TTTGTGCGCT CTATAATGCA
4981 GTCTCTTGAT AACTTTTTGC ACTGTAGGTC CGTTAAGGTT AGAAGAAGGC TACTTTGGTG
5041 TCTATTTTCT CTTCCATAAA AAAAGCCTGA CTCCACTTCC CGCGTTTACT GATTACTAGC
5101 GAAGCTGCGG GTGCATTTTT TCAAGATAAA GGCATCCCCG ATTATATTCT ATACCGATGT
5161 GGATTGCGCA TACTTTGTGA ACAGAAAGTG ATAGCGTTGA TGATTCTTCA TTGGTCAGAA
5221 AATTATGAAC GGTTTCTTCT ATTTTGTCTC TATATACTAC GTATAGGAAA TGTTTACATT
5281 TTCGTATTGT TTTCGATTCA CTCTATGAAT AGTTCTTACT ACAATTTTTT TGTCTAAAGA
5341 GTAATACTAG AGATAAACAT AAAAAATGTA GAGGTCGAGT TTAGATGCAA GTTCAAGGAG
5401 CGAAAGGTGG ATGGGTAGGT TATATAGGGA TATAGCACAG AGATATATAG CAAAGAGATA
5461 CTTTTGAGCA ATGTTTGTGG AAGCGGTATT CGCAATGGGA AGCTCCACCC CGGTTGATAA
5521 TCAGAAAAGC CCCAAAAACA GGAAGATTGT ATAAGCAAAT ATTTAAATTG TAAACGTTAA
5581 TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACGAATAGCC
5641 CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT
5701 TCCAGTTTCC AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA
5761 AAGGGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG
5821 GTCGAGGTGC CGTAAAGCAG TAAATCGGAA GGGTAAACGG ATGCCCCCAT TTAGAGCTTG
5881 ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGGGC
5941 TAGGGCGGTG GGAAGTGTAG GGGTCACGCT GGGCGTAACC ACCACACCCG CCGCGCTTAA
6001 TGGGGCGCTA CAGGGCGCGT GGGGATGATC CACTAGT
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