| 出品公司: | -- |
|---|---|
| 载体名称: | pBBR1MCS-2, pBBR1MCS2, pBBRMCS2 |
| 质粒类型: | 广宿主蛋白表达或克隆载体质粒，广宿主载体质粒 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 5.1 kb |
| 5' 测序引物及序列: | M13-F: TGTAAAACGACGGCCAGT |
| 3' 测序引物及序列: | M13-R: CAGGAAACAGCTATGAC |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 多种微生物物种，是广宿主质粒。 |
| 备注: |
参考文献： [1] Kovach ME, Phillips RW, Elzer PH, Roop RM, 2nd, Peterson KM. pBBR1MCS: a broad-host-range cloning vector. BioTechniques 1994;16:800-2. [2] Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM, 2nd, et al. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 1995;166:175-6. GenBank sequence：U23751 |
| 产品目录号: | -- |
| 稳定性/瞬表达: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pBBR1MCS-2载体 pBBR1MCS2质粒基本信息
pBBR1MCS-2质粒图谱 pBBR1MCS2载体图谱和pBBR1MCS-2载体序列 pBBR1MCS2质粒序列多克隆位点信息
pBBR1MCS-2质粒 pBBR1MCS2载体简介
pBBR1MCS-2质粒序列 pBBR1MCS2载体序列
LOCUS pBBR1MCS-2 5144 bp DNA circular SYN 10-JAN-2005
DEFINITION Cloning vector pBBR1MCS-2 plasmid pBBR1MCS-2, complete sequence.
ACCESSION U23751
VERSION U23751.1 GI:773412
KEYWORDS .
SOURCE Cloning vector pBBR1MCS-2
ORGANISM Cloning vector pBBR1MCS-2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5144)
AUTHORS Kovach,M.E., Phillips,R.W., Elzer,P.H., Roop,R.M. II and
Peterson,K.M.
TITLE pBBR1MCS: a broad-host-range cloning vector
JOURNAL BioTechniques 16 (5), 800-802 (1994)
PUBMED 8068328
REFERENCE 2 (bases 1 to 5144)
AUTHORS Kovach,M.E., Elzer,P.H., Hill,D.S., Robertson,G.T., Farris,M.A.,
Roop,R.M. II and Peterson,K.M.
TITLE Four new derivatives of the broad-host-range cloning vector
pBBR1MCS, carrying different antibiotic-resistance cassettes
JOURNAL Gene 166 (1), 175-176 (1995)
PUBMED 8529885
REFERENCE 3 (bases 1 to 5144)
AUTHORS Kovach,M.E., Elzer,P.H., Hill,S., Robertson,G.T., Ferris,M.,
Roop,R.M. and Peterson,K.M.
TITLE Direct Submission
JOURNAL Submitted (29-MAR-1995) Michael E. Kovach, Microbiology, Louisiana
State University Medical Center, 1501 Kings Highway, Shreveport, LA
71130, USA
FEATURES Location/Qualifiers
source 1..5144
/organism="Cloning vector pBBR1MCS-2"
/mol_type="genomic DNA"
/db_xref="taxon:39477"
/lab_host="Bordetella bronchiseptica"
/plasmid="pBBR1MCS-2"
/note="modification of cloning vector pBBR1MCS, GenBank
Accession Number U02374; gene encoding chloramphenical
resistance replaced by gene encoding kanamycin resistance"
gene complement(join(4945..5144,1..799))
/gene="mob"
CDS complement(join(4945..5144,1..799))
/gene="mob"
/function="required for plasmid mobilization"
/codon_start=1
/transl_table=11
/product="Mob"
/protein_id="AAB05991.1"
/db_xref="GI:773416"
gene 1793..2455
/gene="rep"
CDS 1793..2455
/gene="rep"
/function="required for plasmid replication"
/codon_start=1
/transl_table=11
/product="Rep"
/protein_id="AAB05988.1"
/db_xref="GI:773413"
gene complement(3013..3378)
/gene="lacZ"
CDS complement(3013..3378)
/gene="lacZ"
/note="alpha peptide of LacZ"
/codon_start=1
/transl_table=11
/product="beta galactosidase"
/protein_id="AAB05989.1"
/db_xref="GI:773414"
CDS complement(3893..4687)
/codon_start=1
/transl_table=11
/product="kanamycin resistance determinant"
/protein_id="AAB05990.1"
/db_xref="GI:773415"
ORIGIN
1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
3241 tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga
3301 cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
3361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
3421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
3481 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
3541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcata
3601 aaaactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
3661 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
3721 gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc
3781 cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt
3841 gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga
3901 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
3961 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
4021 acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
4081 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat
4141 cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
4201 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
4261 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
4321 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
4381 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
4441 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
4501 cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
4561 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
4621 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
4681 caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc ctgcgccatc
4741 agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag
4801 agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct
4861 atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg
4921 tccagatagc ccagtagctg acattcatcc caggtggcac ttttcgggga aatgtgcgcg
4981 cccgcgttcc tgctggcgct gggcctgttt ctggcgctgg acttcccgct gttccgtcag
5041 cagcttttcg cccacggcct tgatgatcgc ggcggccttg gcctgcatat cccgattcaa
5101 cggccccagg gcgtccagaa cgggcttcag gcgctcccga aggt
//
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