| 出品公司: | OligoEngine |
|---|---|
| 载体名称: | pSUPER.neo+gfp |
| 质粒类型: | 哺乳动物表达,RNAi |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 多克隆位点 |
| 启动子: | H1 |
| 载体大小: | 5429 bp |
| 5' 测序引物及序列: | T7: AATACGACTCACTATAG |
| 3' 测序引物及序列: | M13-R: CATGGTCATAGCTGTT |
| 载体标签: | EGFP |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neo |
| 克隆菌株: | DH5a |
| 宿主细胞（系）: | 293T细胞等 |
| 备注: | -- |
| 产品目录号: | VEC-PBS-0005/0006 |
| 稳定性: | -- |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 病毒 |
买家导航
pSUPER.neo+gfp载体质粒基本信息
pSUPER.neo+gfp质粒图谱载体图谱和pSUPER.neo+gfp载体序列质粒序列多克隆位点信息
pSUPER.neo+gfp质粒载体简介
pSUPER.neo+gfp质粒序列载体序列
LOCUS pSUPER.neo+GFP 5429 bp DNA SYN
DEFINITION pSUPER.neo+GFP
ACCESSION
KEYWORDS
SOURCE www.biofeng.com
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5429
/organism="pSUPER.neo+GFP"
/mol_type="other DNA"
rep_origin complement(135..441)
/label="f1_origin"
misc_feature complement(459..614)
/label="lacZ_a"
misc_feature 585..607
/label="M13_pUC_fwd_primer"
promoter 600..616
/label="M13_forward20_primer"
promoter 626..644
/label="T7_promoter"
misc_feature 677..693
/label="pBluescriptSK_primer"
terminator complement(731..779)
/label="synth_PA_terminator"
CDS 792..1328
/label="ORF frame 3"
CDS complement(843..1637)
/label="ORF frame 1"
CDS complement(1680..2417)
/label="ORF frame 1"
gene complement(1701..2414)
/label="EGFP"
/gene="EGFP"
misc_feature complement(1743..1764)
/label="EGFP_C_primer"
misc_feature complement(2205..2234)
/label="Y66 (EGFP)"
misc_feature 2351..2372
/label="EGFP_N_primer"
misc_feature complement(2500..2519)
/label="mPGK_F_primer"
misc_feature 2897..2920
/label="MSCV_rev_primer"
misc_feature 3101..3120
/label="H1_primer"
misc_feature complement(3196..3212)
/label="pBluescriptKS_primer"
promoter complement(3240..3259)
/label="T3_promoter"
promoter complement(3276..3294)
/label="M13_reverse_primer"
misc_feature complement(3293..3315)
/label="M13_pUC_rev_primer"
promoter complement(3329..3358)
/label="lac_promoter"
gene complement(4441..5301)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(4441..5301)
/label="ORF frame 3"
promoter complement(5343..5371)
/label="AmpR_promoter"
ORIGIN
1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG
481 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG
541 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG
601 TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT TGGAGCTCCA
661 CCGCGGTGGC GGCCGCTCTA GAACTAGTGG ATCCCCCGGG CTGCATCCCC GATCCTTATC
721 GCTATCGATT CACACAAAAA ACCAACACAC AGATGTAATG AAAATAAAGA TATTTTATTG
781 CGGCCATCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC
841 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG
901 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA
961 CGGGTAGCCA ACGCTATGTC CTGATAGCGG TCCGCCACAC CCAGCCGGCC ACAGTCGATG
1021 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC
1081 ACGACGAGAT CCTCGCCGTC GGGCATGCGC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC
1141 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA
1201 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA
1261 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG
1321 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT
1381 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC
1441 CGCGCTGCCT CGTCCTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA
1501 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT
1561 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT
1621 CCATCTTGTT CAATCATGGT GGCTCTAGCC TTAAGTTCGA GACTGTTGTG TCAGAAGAAT
1681 CAAGCTGATC TGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG
1741 GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGGGCG
1801 GACTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG
1861 TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG GCGGATCTTG
1921 AAGTTCACCT TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT GTGGCTGTTG
1981 TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC
2041 AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG
2101 TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC
2161 TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGC TGAAGCACTG CACGCCGTAG
2221 GTCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAAC
2281 TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC GCTGAACTTG
2341 TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA CCACCCCGGT GAACAGCTCC
2401 TCGCCCTTGC TCACCATGGT GGCGACCGGT AAGCTAGCTT GGGCTGCAGG TCGAAAGGCC
2461 CGGAGATGAG GAAGAGGAGA ACAGCGCGGC AGACGTGCGC TTTTGAAGCG TGCAGAATGC
2521 CGGGCCTCCG GAGGACCTTC GGGCGCCCGC CCCGCCCCTG AGCCCGCCCC TGAGCCCGCC
2581 CCCGGACCCA CCCCTTCCCA GCCTCTGAGC CCAGAAAGCG AAGGAGCAAA GCTGCTATTG
2641 GCCGCTGCCC CAAAGGCCTA CCCGCTTCCA TTGCTCAGCG GTGCTGTCCA TCTGCACGAG
2701 ACTAGTGAGA CGTGCTACTT CCATTTGTCA CGTCCTGCAC GACGCGAGCT GCGGGGCGGG
2761 GGGGAACTTC CTGACTAGGG GAGGAGTAGA AGGTGGCGCG AAGGGGCCAC CAAAGAACGG
2821 AGCCGGTTGG CGCCTACCGG CCGGTGGATG TGGAATGTGT GCGAGGCCAG AGGCCACTTG
2881 TGTAGCGCCA AGTGCCCAGC GGGGCTGCTA AAGCGCATGC TCCAGACTGC CTTGGGAAAA
2941 GCGCCTCCCC TACCCGGTAG AATTCGAACG CTGACGTCAT CAACCCGCTC CAAGGAATCG
3001 CGGGCCCAGT GTCACTAGGC GGGAACACCC AGCGCGCGTG CGCCCTGGCA GGAAGATGGC
3061 TGTGAGGGAC AGGGGAGTGG CGCCCTGCAA TATTTGCATG TCGCTATGTG TTCTGGGAAA
3121 TCACCATAAA CGTGAAATGT CTTTGGATTT GGGAATCTTA TAAGTTCTGT ATGAGACCAC
3181 AGATCTAAGC TTATCGATAC CGTCGACCTC GAGGGGGGGC CCGGTACCCA GCTTTTGTTC
3241 CCTTTAGTGA GGGTTAATTG CGCGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG
3301 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC
3361 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT
3421 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG
3481 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT
3541 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC
3601 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA
3661 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
3721 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
3781 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
3841 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
3901 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
3961 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
4021 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
4081 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG
4141 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
4201 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
4261 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
4321 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
4381 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
4441 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
4501 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
4561 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
4621 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
4681 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
4741 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
4801 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
4861 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
4921 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
4981 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
5041 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
5101 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
5161 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
5221 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
5281 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
5341 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
5401 TCCGCGCACA TTTCCCCGAA AAGTGCCAC
//
pSUPER.neo+gfp载体图谱质粒图谱pdf版和pSUPER.neo+gfp载体序列质粒序列等相关资料下载
pSUPER.neo+gfp质粒载体应用举例
- 上一篇：pTagBFP-N
- 下一篇：pCDNA3.0-HA-N
