| 质粒名称: | c-myc-PT3EF1a |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 92046 |
| 存储实验室: | Xin Chen |
|
载体骨架基本信息 |
|
| 载体名称: | pT3-EF1a |
| 载体抗性: | 氨苄青霉素 |
| 载体类型: | 哺乳细胞表达 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | C-MYC,MYC |
| 物种来源: | H. sapiens (human) |
| GenBank编号 | P01106.1 |
| 启动子 | EF-1a |
| 克隆方法: | Gateway克隆 |
| 5' 测序引物 | GAGTTTGGATCTTGGTTCATTC |
| 3' 测序引物 | TAGAAGGCACAGTCGAGG |
|
文献引用方法 |
|
| 材料和方法部分: | c-myc-PT3EF1a was a gift from Xin Chen (Addgene plasmid # 92046 ; http://n2t.net/addgene:92046 ; RRID:Addgene_92046) |
| 参考文献部分: | A functional mTORC1 signaling is indispensable for c-Myc driven hepatocarcinogenesis. Liu P, Ge M, Hu J, Li X, Che L, Sun K, Cheng L, Huang Y, Pilo MG, Cigliano A, Pes GM, Pascale RM, Brozzetti S, Vidili G, Porcu A, Cossu A, Palmieri G, Sini MC, Ribback S, Dombrowski F, Tao J, Calvisi DF, Chen L, Chen X. Hepatology. 2017 Mar 30. doi: 10.1002/hep.29183. 10.1002/hep.29183 PubMed 28370287 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS c-myc-PT3EF1a 6494 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses c-Myc in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS c-myc-PT3EF1a
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6494)
AUTHORS Liu P, Ge M, Hu J, Li X, Che L, Sun K, Cheng L, Huang Y, Pilo MG,
Cigliano A, Pes GM, Pascale RM, Brozzetti S, Vidili G, Porcu A,
Cossu A, Palmieri G, Sini MC, Ribback S, Dombrowski F, Tao J,
Calvisi DF, Chen L, Chen X
TITLE A functional mTORC1 signaling is indispensable for c-Myc driven
hepatocarcinogenesis.
JOURNAL Hepatology. 2017 Mar 30. doi: 10.1002/hep.29183.
PUBMED 28370287
REFERENCE 2 (bases 1 to 6494)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6494
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..1179
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 232..1170
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1127..1147
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
protein_bind 1210..1234
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 1268..2584
/codon_start=1
/product="human c-Myc proto-oncogene"
/label=c-Myc
/translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
protein_bind complement(2601..2625)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
primer_bind 2653..2672
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(2707..2724)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2713..2937
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(3355..3371)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3355..3371)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3368..3390)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3379..3395
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3403..3433)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3448..3469
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3586..3603)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3757..4345)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3837..3856)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4516..5376)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5139..5158
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5377..5481)
/gene="bla"
/label=AmpR promoter
primer_bind 5549..5567
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(5605..5627)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 5727..5746
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5940..5962
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 5954..5971
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 5955..5971
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6353..6373)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtaccaata
1201 cggatatcaa caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg
1261 atccaccatg cccctcaacg ttagcttcac caacaggaac tatgacctcg actacgactc
1321 ggtgcagccg tatttctact gcgacgagga ggagaacttc taccagcagc agcagcagag
1381 cgagctgcag cccccggcgc ccagcgagga tatctggaag aaattcgagc tgctgcccac
1441 cccgcccctg tcccctagcc gccgctccgg gctctgctcg ccctcctacg ttgcggtcac
1501 acccttctcc cttcggggag acaacgacgg cggtggcggg agcttctcca cggccgacca
1561 gctggagatg gtgaccgagc tgctgggagg agacatggtg aaccagagtt tcatctgcga
1621 cccggacgac gagaccttca tcaaaaacat catcatccag gactgtatgt ggagcggctt
1681 ctcggccgcc gccaagctcg tctcagagaa gctggcctcc taccaggctg cgcgcaaaga
1741 cagcggcagc ccgaaccccg cccgcggcca cagcgtctgc tccacctcca gcttgtacct
1801 gcaggatctg agcgccgccg cctcagagtg catcgacccc tcggtggtct tcccctaccc
1861 tctcaacgac agcagctcgc ccaagtcctg cgcctcgcaa gactccagcg ccttctctcc
1921 gtcctcggat tctctgctct cctcgacgga gtcctccccg cagggcagcc ccgagcccct
1981 ggtgctccat gaggagacac cgcccaccac cagcagcgac tctgaggagg aacaagaaga
2041 tgaggaagaa atcgatgttg tttctgtgga aaagaggcag gctcctggca aaaggtcaga
2101 gtctggatca ccttctgctg gaggccacag caaacctcct cacagcccac tggtcctcaa
2161 gaggtgccac gtctccacac atcagcacaa ctacgcagcg cctccctcca ctcggaagga
2221 ctatcctgct gccaagaggg tcaagttgga cagtgtcaga gtcctgagac agatcagcaa
2281 caaccgaaaa tgcaccagcc ccaggtcctc ggacaccgag gagaatgtca agaggcgaac
2341 acacaacgtc ttggagcgcc agaggaggaa cgagctaaaa cggagctttt ttgccctgcg
2401 tgaccagatc ccggagttgg aaaacaatga aaaggccccc aaggtagtta tccttaaaaa
2461 agccacagca tacatcctgt ccgtccaagc agaggagcaa aagctcattt ctgaagagga
2521 cttgttgcgg aaacgacgag aacagttgaa acacaaactt gaacagctac ggaactcttg
2581 tgcggactcg agatatctag acccagcttt cttgtacaaa gtggttgata tcgcggccgc
2641 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct
2701 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
2761 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
2821 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
2881 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
2941 ctgaggcggg atccccttga aatacatcca caggtacacc tccaattgac tcaaatgatg
3001 tcaattagtc tatcagaagc ttctaaagcc atgacatcat tttctggaat tttccaagct
3061 gtttaaaggc acagtcaact tagtgtatgt aaacttctga cccactggaa ttgtgataca
3121 gtgaattata agtgaaataa tctgtctgta aacaattgtt ggaaaaatga cttgtgtcat
3181 gcacaaagta gatgtcctaa ctgacttgcc aaaactattg tttgttaaca agaaatttgt
3241 ggagtagttg aaaaacgagt tttaatgact ccaacttaag tgtatgtaaa cttccgactt
3301 caactgtata ggtctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata
3361 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
3421 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
3481 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
3541 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
3601 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
3661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
3721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
3781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
3841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
3901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
3961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
4021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
4081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
4141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
4201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
4261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
4321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
4381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
4441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
4501 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
4561 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
4621 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
4681 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
4741 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
4801 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
4861 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
4921 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
4981 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
5041 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
5101 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
5161 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
5221 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
5281 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
5341 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
5401 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
5461 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
5521 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
5581 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
5641 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
5701 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
5761 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
5821 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
5881 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
5941 ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctcggtaccc tacagttgaa
6001 gtcggaagtt tacatacact taagttggag tcattaaaac tcgtttttca actactccac
6061 aaatttcttg ttaacaaaca atagttttgg caagtcagtt aggacatcta ctttgtgcat
6121 gacacaagtc atttttccaa caattgttta cagacagatt atttcactta taattcactg
6181 tatcacaatt ccagtgggtc agaagtttac atacactaag ttgactgtgc ctttaaacag
6241 cttggaaaat tccagaaaat gatgtcatgg ctttagaagc ttctgataga ctaattgaca
6301 tcatttgagt caattggagg tgtacctgtg gatgtatttc aaggaattct gtggaatgtg
6361 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
6421 catatcgata ctagtttaat taagatcctc tttgcagcta atggaccttc taggtcttga
6481 aaggagtggg aatt
//
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