pBad/gIII A

价格：1000元

联系方式：I47-825O-882O

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pBad/gIII A载体 pBadgIII A质粒基本信息

出品公司:	Invitrogen
载体名称:	pBad/gIII A， pBadgIII A, pBadgIIIA. pBad/gIIIA
质粒类型:	大肠杆菌蛋白表达载体
表达水平:	高
启动子:	araBad
克隆方法:	多克隆位点，限制性内切酶
载体大小:	4145 bp
5' 测序引物及序列:	pBAD-F: ATGCCATAGCATTTTTATCC
3' 测序引物及序列:	pBAD-R: gatttaatctgtatcagg
载体标签:	N-GIII signal, C-His, C-Myc
载体抗性:	氨苄
备注:	--
产品目录号:	V450-01
稳定性:	瞬时表达 Transient
组成型:	诱导表达
病毒/非病毒:	非病毒

pBad/gIII A质粒图谱 pBadgIII A载体图谱和pBad/gIII A载体序列 pBadgIII A质粒序列多克隆位点信息

pBad/gIII A载体图谱

pBad/gIII A质粒 pBadgIII A载体简介

pBAD/gIII载体质粒是衍生于pBR322载体。载体设计用来在大肠杆菌中进行可调节分泌性表达和纯化重组目的蛋白。使用基因III信号序列来定位重组蛋白，使其分泌到大肠杆菌周质腔中。使用大肠杆菌araBAD启动子（pBAD）增强了大肠杆菌重组蛋白可溶性表达的水平。载体上的调节蛋白AraC能够调控pBad启动子。

pBad/gIII A质粒序列 pBadgIII A载体序列

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCA
AACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACA
AAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAG
CATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACC
CGTTTTTTGGGCTAACAGGAGGAATTAACCATGAAAAAACTGCTGTTCGCGATTCCGCTGGTGGTGCCGTTC
TATAGCCATAGCACCATGGAGCTCGAGATCTGCAGCTGGTACCATATGGGAATTCGAAGCTTTCTAGAACAA
AAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTC
TCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCG
GTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAG
TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATA
AAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT
AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCG
CCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACT
CTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTC
AATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCAT
TTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCAC
GAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTC
CAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAAC
TCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGG
ATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTC
TGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTG
ATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGG
CAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGA
TGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAAT
CTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCG
TAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCT
CACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATT
TTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTT
CGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAA
TCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTC
TTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAG
GCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTG
CCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGG
GCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGC
GTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCG
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCC
ACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACG
CGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATT
CTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCG
AGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCAC
ACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATC
GCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCT
GCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTC
ATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCA
AATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAA
TTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCC
GGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGAT
AGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCT
AATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGC
GATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGG
TGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAG
CTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTG
CGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGC
GCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTC
TCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTG
ACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACC
GTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATT
TTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

pMal-c2G	pKLAC1
pcDNA5/FRT/V5-His-TOPO	pCYM1
pMTD1.3xA	pFLAG-CMV2
pMal-c4X	pLLP-OmpA
pAN7-1	pSH65
pHSG422	c-Flag pcDNA3
pPIC9k-His	pRSET-EGFP
pIC20H	pML21
pEF1/V5-HisA	pRB373
pNEO	pCL-Ampho
pUC4-KISS	pLVX-DD-ZsGreen1 Control
pREP4	pET-21a(+)
pBD-NF-kB	pGL4.38[luc2P/p53 RE/Hygro]
pFLAG-CMV-3	pMT-Bip-V5-HisA
pcDNA3.1(+)/CAT	pPIC ZαGB
pPIC9	pET-32 EK/LIC
pDC515	pProLabel-C
pCH2	pUCTag
pGL4.41[luc2P/HSE/Hygro]	pSP72
pCGN978	pACT-MyoD
pBad43	pWF1
Pgex-HRV3C	pENTR11-Dual
pCRE-MetLuc2-Reporter	pcDNA4/HisMax B
pIJ702-del35-55	pBT-4
pHV23	pSUMO
pCas9D10A-GFP	pCRE-DD-AmCyan1
pTi	pSET152
pcDNA6.2-DsRed2-MCS1 miR	pcDNA5/FRT
pAcGFP1-N2	pIκB-EGFP
pET-28a-BFP-C	pNEO-ars
pVPack-GP	pALEX a,b,c
EWD299	pAGO
pSC101	pLexm
pAD-SV40T	pdKeima-Red-S1
pcDNA3.1-GP130v1	pHB-1
pATPase_TA	pMDLg/pRRE
pUG6	pGL4.72[hRlucCP]
pHS85	pHY15
PX543	pBAD18
pFA1	pOM2
pDEST26	pTet-Splice
pJSC73	pTP-4
pOrf681_TA	pET-41a(+)
pEF6/myc-His B	pET-32a(+)
ISceI-GR-RFP	pSC101-EcoRImeth+
pLNCX2	pCDH-CMV-MCS-EF1a-RFP+BSD
pSUPER.neo+gfp	pGL4.45[luc2P/ISRE/Hygro]
pBluescript II KS(-)	pET-12c(+)
pCREj-2	pL-brevisGH3-MBP
pBBR1MCS-2	pDsRed-Monomer

pNN9	BECLIN-HA WT
pcDNA3.4_7C11-1 heavy chain	pLenti X2 Puro DEST (w16-1)
p58sheLL	pICH86966::AtU6p::sgRNA_PDS
pAAVS1-PC-GCaMP6f	pFRT/TO/GFP-SCAF4 (CRISPR_resistant)
pNeae2	pMSCV-IRES-GFP II (pMIG II)
pM1s3AsR_TS-C7	AbVec1.1-IGKC
pLVX-M-puro	800 pSG5L HA PTEN wt
pQUAST>stop>mCD8-GFP	pUCC001
VVT1	LeGO-G
pGH335_MS2-AID*Δ-Hygro	pBHt2
ilux pGEX(-)	pNK9
PB_tre_Cas9	pCS2TAL3-RR
pGL3 2 kb prom. CD274	pHAGE-PTCH1
pT3-Neo-EF1a-GFP	pJKR-L-tetR
pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV	pAAV2/8
pGGA008	pRC-CMV-Rev1b
pLenti-EML4-ALK variant 3a	pNEU-hMbPylRS-4xU6M15
FUmGW	pYES-mtGFP
pLenti-DsRed_IRES_MAPT:EGFP	pSELECT-HA-mFOXO1
pMJ329: pCMV-MmeFz2	pTEF:ATP
pAAV-EFS-CjCas9-eGFP-HIF1a	pC003 - LshC2C2 locus into pACYC184 for
pEGFP-LC3	c-myc-PT3EF1a
pTRE Tight ArchT-GFP	pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-
pDIRECT_23D	Lenti-luciferase-P2A-Neo
401 pSG5L	pJSC131 - Bacterial expression plasmid f
phage UbiC NLS HA stdMCP stdHalo	EC2_10_dCas9_VPR_HC_sgRNA
pMSCV-hygro-STING	pAc-sgRNA-Cas9
pCDH-puro-Bcl-XL	LB1366 (Bcl-2 promoter with MHS1234)
pEGFP-puro	pMXs-hOCT4 (Plath)
PVALB mAb_BCN14.1.1A2_HC_Mouse IgG2a	pMonAID_nCas9-PmCDA_Hyg_ALS
pSBtet-GP	pLJC5-3XHA-EGFP-PEX26
GST-HA-GFP11-N1	pSR47
pFC334	Cbh_v5 AAV-CBE N-terminal
pLentiV2-dCas9-VP64	pRAD1
pM-Cas9	pAc-Neo-OVA
1306 pSG5L HA FKHR wt	EC1000
pAC1404-pCR8-mRuby2_NLSPUFa	pCW-Cas9
pCMV-MMLVgag-3xNES-ABE8e	pTRKH2
pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-I	pCAS9-mCherry empty
MSCV-(pBabe mcs)- human p210BCR-ABL-IRES	pXAT2
pMal-T-Avi-His/BirA	pAraGFPCDF
p201G Cas9	pPPEM
NM9-SpCas9-NLS3	3XMEF2-luc
EC2_3_dCas9_Mxi1_sgRNA	pET28a-At-TRL
pCMV-hA3G-BE3	Tet-O-FUW-Neurod1
CKS2 gRNA (BRDN0001149143)	TAL3393
pCXLE-hOCT3/4	pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK
BPK3082	pT4-CMV-GFP
pCAG-GBP1-10gly-lexADBD	pAAV-EF1a-FLEX-GTB
pCS2TAL3-DD	pAAVS1-PDi-CRISPRn

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pBad/gIII A

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pBad/gIII A载体 pBadgIII A质粒基本信息

pBad/gIII A质粒图谱 pBadgIII A载体图谱和pBad/gIII A载体序列 pBadgIII A质粒序列多克隆位点信息

pBad/gIII A质粒 pBadgIII A载体简介

pBad/gIII A质粒序列 pBadgIII A载体序列

pBad/gIII A载体图谱 pBadgIII A质粒图谱pdf版和pBad/gIII A载体序列 pBadgIII A质粒序列等相关资料下载

pBad/gIII A质粒 pBadgIII A载体应用举例

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