| 质粒名称: | pHAGE-PTCH1 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 116779 |
| 存储实验室: | Gordon Mills, Kenneth Scott |
|
载体骨架基本信息 |
|
| 载体名称: | pHAGE |
| 完整质粒大小: | 12857 bp |
| 载体类型: | Lentiviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | PTCH1 |
| 物种来源: | H. sapiens (human) |
| 基因突变: | WT |
| 克隆方法: | Gateway Cloning |
|
文献引用方法 |
|
| 材料和方法部分: | pHAGE-PTCH1 was a gift from Gordon Mills & Kenneth Scott (Addgene plasmid # 116779 ; http://n2t.net/addgene:116779 ; RRID:Addgene_116779) |
| 参考文献部分: | Systematic Functional Annotation of Somatic Mutations in Cancer. Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB. Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. 10.1016/j.ccell.2018.01.021 PubMed 29533785 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHAGE-PTCH1 12857 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral expression of PTCH1.
ACCESSION .
VERSION .
KEYWORDS pHAGE-PTCH1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12857)
AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K,
Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip
CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R,
Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott
KL, Yi S, Sahni N, Liang H, Mills GB
TITLE Systematic Functional Annotation of Somatic Mutations in Cancer.
JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi:
10.1016/j.ccell.2018.01.021.
PUBMED 29533785
REFERENCE 2 (bases 1 to 12857)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..12857
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2058..2175
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2223..3401
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2454..3392
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3349..3369
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
protein_bind 3421..3445
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(7817..7841)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
promoter 7849..7867
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(7892..7911)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(7893..7911)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind complement(7917..7937)
/gene="mutant version of attB"
/label=attB4
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
CDS 7991..8032
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
misc_feature 8053..8620
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 8070..8622
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(8214..8231)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 8441..8460
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 8634..9233
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(8634..8653)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 9130..9150
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 9247..9835
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(9300..9320)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(9718..9729)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 9910..10143
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(10262..10280)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 10348..10452
/gene="bla"
/label=AmpR promoter
CDS 10453..11313
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(10671..10690)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 11484..12072
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 11973..11992
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 12226..12243
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 12335..12354
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 12483..12504
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 12519..12549
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 12557..12573
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 12562..12584
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 12581..12597
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 12581..12597
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 12647..12666
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
3421 acaagtttgt acaaaaaagc agaagggccg tcaaggccca ccatggcctc ggctggtaac
3481 gccgccgagc cccaggaccg cggcggcggc ggcagcggct gtatcggtgc cccgggacgg
3541 ccggctggag gcgggaggcg cagacggacg ggggggctgc gccgtgctgc cgcgccggac
3601 cgggactatc tgcaccggcc cagctactgc gacgccgcct tcgctctgga gcagatttcc
3661 aaggggaagg ctactggccg gaaagcgccg ctgtggctga gagcgaagtt tcagagactc
3721 ttatttaaac tgggttgtta cattcaaaaa aactgcggca agttcttggt tgtgggcctc
3781 ctcatatttg gggccttcgc ggtgggatta aaagcagcga acctcgagac caacgtggag
3841 gagctgtggg tggaagttgg aggacgagta agtcgtgaat taaattatac tcgccagaag
3901 attggagaag aggctatgtt taatcctcaa ctcatgatac agacccctaa agaagaaggt
3961 gctaatgtcc tgaccacaga agcgctccta caacacctgg actcggcact ccaggccagc
4021 cgtgtccatg tatacatgta caacaggcag tggaaattgg aacatttgtg ttacaaatca
4081 ggagagctta tcacagaaac aggttacatg gatcagataa tagaatatct ttacccttgt
4141 ttgattatta cacctttgga ctgcttctgg gaaggggcga aattacagtc tgggacagca
4201 tacctcctag gtaaacctcc tttgcggtgg acaaacttcg accctttgga attcctggaa
4261 gagttaaaga aaataaacta tcaagtggac agctgggagg aaatgctgaa taaggctgag
4321 gttggtcatg gttacatgga ccgcccctgc ctcaatccgg ccgatccaga ctgccccgcc
4381 acagccccca acaaaaattc aaccaaacct cttgatatgg cccttgtttt gaatggtgga
4441 tgtcatggct tatccagaaa gtatatgcac tggcaggagg agttgattgt gggtggcaca
4501 gtcaagaaca gcactggaaa actcgtcagc gcccatgccc tgcagaccat gttccagtta
4561 atgactccca agcaaatgta cgagcacttc aaggggtacg agtatgtctc acacatcaac
4621 tggaacgagg acaaagcggc agccatcctg gaggcctggc agaggacata tgtggaggtg
4681 gttcatcaga gtgtcgcaca gaactccact caaaaggtgc tttccttcac caccacgacc
4741 ctggacgaca tcctgaaatc cttctctgac gtcagtgtca tccgcgtggc cagcggctac
4801 ttactcatgc tcgcctatgc ctgtctaacc atgctgcgct gggactgctc caagtcccag
4861 ggtgccgtgg ggctggctgg cgtcctgctg gttgcactgt cagtggctgc aggactgggc
4921 ctgtgctcat tgatcggaat ttcctttaac gctgcaacaa ctcaggtttt gccatttctc
4981 gctcttggtg ttggtgtgga tgatgttttt cttctggccc acgccttcag tgaaacagga
5041 cagaataaaa gaatcccttt tgaggacagg accggggagt gcctgaagcg cacaggagcc
5101 agcgtggccc tcacgtccat cagcaatgtc acagccttct tcatggccgc gttaatccca
5161 attcccgctc tgcgggcgtt ctccctccag gcagcggtag tagtggtgtt caattttgcc
5221 atggttctgc tcatttttcc tgcaattctc agcatggatt tatatcgacg cgaggacagg
5281 agactggata ttttctgctg ttttacaagc ccctgcgtca gcagagtgat tcaggttgaa
5341 cctcaggcct acaccgacac acacgacaat acccgctaca gccccccacc tccctacagc
5401 agccacagct ttgcccatga aacgcagatt accatgcagt ccactgtcca gctccgcacg
5461 gagtacgacc cccacacgca cgtgtactac accaccgctg agccgcgctc cgagatctct
5521 gtgcagcccg tcaccgtgac acaggacacc ctcagctgcc agagcccaga gagcaccagc
5581 tccacaaggg acctgctctc ccagttctcc gactccagcc tccactgcct cgagcccccc
5641 tgtacgaagt ggacactctc atcttttgct gagaagcact atgctccttt cctcttgaaa
5701 ccaaaagcca aggtagtggt gatcttcctt tttctgggct tgctgggggt cagcctttat
5761 ggcaccaccc gagtgagaga cgggctggac cttacggaca ttgtacctcg ggaaaccaga
5821 gaatatgact ttattgctgc acaattcaaa tacttttctt tctacaacat gtatatagtc
5881 acccagaaag cagactaccc gaatatccag cacttacttt acgacctaca caggagtttc
5941 agtaacgtga agtatgtcat gttggaagaa aacaaacagc ttcccaaaat gtggctgcac
6001 tacttcagag actggcttca gggacttcag gatgcatttg acagtgactg ggaaaccggg
6061 aaaatcatgc caaacaatta caagaatgga tcagacgatg gagtccttgc ctacaaactc
6121 ctggtgcaaa ccggcagccg cgataagccc atcgacatca gccagttgac taaacagcgt
6181 ctggtggatg cagatggcat cattaatccc agcgctttct acatctacct gacggcttgg
6241 gtcagcaacg accccgtcgc gtatgctgcc tcccaggcca acatccggcc acaccgacca
6301 gaatgggtcc acgacaaagc cgactacatg cctgaaacaa ggctgagaat cccggcagca
6361 gagcccatcg agtatgccca gttccctttc tacctcaacg gcttgcggga cacctcagac
6421 tttgtggagg caattgaaaa agtaaggacc atctgcagca actatacgag cctggggctg
6481 tccagttacc ccaacggcta ccccttcctc ttctgggagc agtacatcgg cctccgccac
6541 tggctgctgc tgttcatcag cgtggtgttg gcctgcacat tcctcgtgtg cgctgtcttc
6601 cttctgaacc cctggacggc cgggatcatt gtgatggtcc tggcgctgat gacggtcgag
6661 ctgttcggca tgatgggcct catcggaatc aagctcagtg ccgtgcccgt ggtcatcctg
6721 atcgcttctg ttggcatagg agtggagttc accgttcacg ttgctttggc ctttctgacg
6781 gccatcggcg acaagaaccg cagggctgtg cttgccctgg agcacatgtt tgcacccgtc
6841 ctggatggcg ccgtgtccac tctgctggga gtgctgatgc tggcgggatc tgagttcgac
6901 ttcattgtca ggtatttctt tgctgtgctg gcgatcctca ccatcctcgg cgttctcaat
6961 gggctggttt tgcttcccgt gcttttgtct ttctttggac catatcctga ggtgtctcca
7021 gccaacggct tgaaccgcct gcccacaccc tcccctgagc caccccccag cgtggtccgc
7081 ttcgccatgc cgcccggcca cacgcacagc gggtctgatt cctccgactc ggagtatagt
7141 tcccagacga cagtgtcagg cctcagcgag gagcttcggc actacgaggc ccagcagggc
7201 gcgggaggcc ctgcccacca agtgatcgtg gaagccacag aaaaccccgt cttcgcccac
7261 tccactgtgg tccatcccga atccaggcat cacccaccct cgaacccgag acagcagccc
7321 cacctggact cagggtccct gcctcccgga cggcaaggcc agcagccccg cagggacccc
7381 cccagagaag gcttgtggcc acccccctac agaccgcgca gagacgcttt tgaaatttct
7441 actgaagggc attctggccc tagcaatagg gcccgctggg gccctcgcgg ggcccgttct
7501 cacaaccctc ggaacccagc gtccactgcc atgggcagct ccgtgcccgg ctactgccag
7561 cccatcacca ctgtgacggc ttctgcctcc gtgactgtcg ccgtgcaccc gccgcctgtc
7621 cctgggcctg ggcggaaccc ccgaggggga ctctgcccag gctaccctga gactgaccac
7681 ggcctgtttg aggaccccca cgtgcctttc cacgtccggt gtgagaggag ggattcgaag
7741 gtggaagtca ttgagctgca ggacgtggaa tgcgaggaga ggccccgggg aagcagctcc
7801 aactcaggcc tcatgggccc agctttcttg tacaaagtgg tgatatccaa ttaaccctca
7861 ctaaaggtta agtgaagtgt tgtttattga accctatagt gagtcgtatt accacccaac
7921 ttttctatac aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc
7981 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
8041 ttagtaatga gatccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
8101 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
8161 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
8221 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
8281 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
8341 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
8401 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
8461 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
8521 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
8581 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca catatgaccg
8641 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
8701 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
8761 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
8821 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
8881 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
8941 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
9001 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
9061 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
9121 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
9181 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc taaatcgata
9241 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
9301 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
9361 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
9421 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
9481 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
9541 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
9601 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
9661 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
9721 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
9781 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag
9841 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
9901 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
9961 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
10021 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
10081 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
10141 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
10201 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag
10261 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
10321 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
10381 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
10441 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
10501 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
10561 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
10621 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
10681 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
10741 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
10801 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
10861 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
10921 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
10981 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
11041 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
11101 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
11161 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
11221 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
11281 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
11341 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
11401 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
11461 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
11521 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
11581 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
11641 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
11701 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
11761 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
11821 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
11881 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
11941 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
12001 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
12061 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
12121 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
12181 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
12241 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
12301 taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
12361 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
12421 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
12481 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
12541 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
12601 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
12661 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
12721 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
12781 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
12841 actacaaact tagtagt
//
