pLVX-EF1a-tetOn-IRES-G418 (EtO)

LOCUS       pLVX-EF1a-tetOn-IRES-G418 (EtO)               12247 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Second generation lenti tetracycline operator (on).
ACCESSION   .
VERSION     .
KEYWORDS    pLVX-EF1a-tetOn-IRES-G418 (EtO)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12247)
  AUTHORS   Mertens J, Paquola AC, Ku M, Hatch E, Bohnke L, Ladjevardi S, 
            McGrath S, Campbell B, Lee H, Herdy JR, Goncalves JT, Toda T, Kim Y,
            Winkler J, Yao J, Hetzer MW, Gage FH
  TITLE     Directly Reprogrammed Human Neurons Retain Aging-Associated 
            Transcriptomic Signatures and Reveal Age-Related Nucleocytoplasmic 
            Defects.
  JOURNAL   Cell Stem Cell. 2015 Oct 6. pii: S1934-5909(15)00408-7. doi: 
            10.1016/j.stem.2015.09.001.
  PUBMED    26456686
REFERENCE   2  (bases 1 to 12247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..12247
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2282..3460
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2513..3451
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3408..3428
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3483..4229
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4349..4811
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4405..4422)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4632..4651
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4825..5619
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4879..4898)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5489..5508
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    5815..6403
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5868..5888)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6286..6297)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6610..7243
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7350..7367)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7356..7580
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7626..8054
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7713..7732)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7923..7944
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8063..8083)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8068..8397
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8248..8383
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8310..8329
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8446..9471
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    9601..9722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9771..9787)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9771..9787)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9784..9806)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9795..9811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9819..9849)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9864..9885
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10002..10019)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10173..10761)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10253..10272)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10932..11792)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11555..11574
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11793..11897)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11945..12079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             12247..633
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgcgcccg acgataagct ttgcaaagat ggataaagtt
     2221 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
     2281 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     2341 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     2401 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     2461 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     2521 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     2581 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
     2641 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
     2701 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
     2761 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
     2821 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
     2881 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     2941 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     3001 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     3061 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     3121 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     3181 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     3241 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     3301 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     3361 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     3421 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaatttcga cccggatcca
     3481 ccatgtctag actggacaag agcaaagtca taaacggcgc tctggaatta ctcaatggag
     3541 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     3601 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     3661 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
     3721 ggaacaacgc caagtcattc cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     3781 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     3841 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     3901 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     3961 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc
     4021 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     4081 agctaaagtg cgaaagcggc gggccggccg acgcccttga cgattttgac ttagacatgc
     4141 tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg
     4201 attttgacct tgacatgctc cccgggtaac tagaattccg cccccccccc ccccccccta
     4261 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     4321 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     4381 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     4441 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     4501 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     4561 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     4621 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     4681 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     4741 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     4801 cacgatgata agcttgccac aaccatggct gaacaagatg gattgcacgc aggttctccg
     4861 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     4921 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     4981 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     5041 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     5101 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     5161 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     5221 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     5281 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     5341 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     5401 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     5461 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     5521 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     5581 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggatcggc tagagtcgac
     5641 ccggacggga cgctcctgcg cctgatacag aacgaattgc ttgcaggcat ctcatgagtg
     5701 tgtcttcccg ttttccgcct gaggtcactg cgtggatgga gcgctggcgc ctgctgcgcg
     5761 acggcgagct gctcaccacc cactcgagtc tagagggccc gacgcgtctg gaacaatcaa
     5821 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5881 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5941 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6001 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6061 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6121 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6181 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     6241 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6301 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6361 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca
     6421 gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     6481 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     6541 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6601 agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     6661 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     6721 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     6781 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     6841 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     6901 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     6961 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     7021 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     7081 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7141 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7201 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     7261 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga
     7321 cattgctagc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     7381 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     7441 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     7501 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     7561 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     7621 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     7681 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     7741 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     7801 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     7861 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     7921 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     7981 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     8041 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     8101 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     8161 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8221 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     8281 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     8341 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     8401 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgatgaa aaagcctgaa
     8461 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     8521 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     8581 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     8641 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
     8701 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     8761 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     8821 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     8881 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     8941 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     9001 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     9061 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     9121 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     9181 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     9241 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     9301 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     9361 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     9421 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtgcta
     9481 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     9541 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     9601 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     9661 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9721 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     9781 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     9841 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     9901 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     9961 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    10021 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    10081 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    10141 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    10201 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    10261 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    10321 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    10381 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    10441 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    10501 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    10561 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    10621 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    10681 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    10741 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    10801 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    10861 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    10921 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10981 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    11041 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    11101 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    11161 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    11221 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    11281 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    11341 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    11401 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    11461 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    11521 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    11581 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    11641 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    11701 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    11761 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    11821 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    11881 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
    11941 gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    12001 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    12061 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
    12121 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
    12181 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
    12241 agtagtt
//