pCG

LOCUS       pCG                5015 bp DNA     circular SYN 12-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Biofeng
  TITLE     Direct Submission
  JOURNAL   Exported Jul 12, 2023 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     intron          704..1276
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    1473..1528
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     promoter        complement(1865..2061)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1879..2014
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2299..2887)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3058..3918)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3919..4023)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4355..4810)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4955..4971
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4978..4996
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        join(5015,1..303)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtatggctt
      661 ctagaattcg agctcggtac ccggggatcc tgagaacttc agggtgagtt tggggaccct
      721 tgattgttct ttctttttcg ctattgtaaa attcatgtta tatggagggg gcaaagtttt
      781 cagggtgttg tttagaatgg gaagatgtcc cttgtatcac catggaccct catgataatt
      841 ttgtttcttt cactttctac tctgttgaca accattgtct cctcttattt tcttttcatt
      901 ttctgtaact ttttcgttaa actttagctt gcatttgtaa cgaattttta aattcacttt
      961 tgtttatttg tcagattgta agtactttct ctaatcactt ttttttcaag gcaatcaggg
     1021 tatattatat tgtacttcag cacagtttta gagaacaatt gttataatta aatgataagg
     1081 tagaatattt ctgcatataa attctggctg gcgtggaaat attcttattg gtagaaacaa
     1141 ctacaccctg gtcatcatcc tgcctttctc tttatggtta caatgatata cactgtttga
     1201 gatgaggata aaatactctg agtccaaacc gggcccctct gctaaccatg ttcatgcctt
     1261 cttctctttc ctacagctcc tgggcaacgt gctggttgtt gtgctgtctc atcattttgg
     1321 caaagaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     1381 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     1441 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     1501 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     1561 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgccatat gctggctgcc
     1621 atgaacaaag gtggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     1681 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     1741 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     1801 tcctctcctg actactccca gtcatagctg tccctcttct cttatgaaga tcctcgagaa
     1861 gctgtttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag
     1921 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag
     1981 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg
     2041 ttgctgacta attgagatgc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     2101 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2161 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2221 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2281 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2341 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2401 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2461 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2521 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2581 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2641 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2701 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     2761 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2821 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2881 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2941 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3001 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3061 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3121 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3181 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3241 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3301 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3361 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3421 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3481 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3541 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3601 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3661 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3721 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3781 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3841 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3901 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3961 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4021 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     4081 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     4141 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     4201 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     4261 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     4321 gcacagatgc gtaaggagaa aataccgcat caggaaattg taaacgttaa tattttgtta
     4381 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     4441 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     4501 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     4561 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     4621 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     4681 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     4741 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     4801 cagggcgcgt cgcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     4861 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     4921 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa
     4981 tacgactcac tatagggcga attcgagctc gcccc
//