| 质粒名称: | pLJC5-3XHA-EGFP-PEX26 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 139054 |
| 存储实验室: | David Sabatini |
|
载体骨架基本信息 |
|
| 载体名称: | pLJC5 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | 3XHA-EGFP-PEX26 |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | UBC |
| 标签/融合蛋白: | HA（插入件上的N端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | EcorI (未破坏) |
| 5' 测序引物 | TGAAGCTCCGGTTTTGAACT |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLJC5-3XHA-EGFP-PEX26 was a gift from David Sabatini (Addgene plasmid # 139054 ; http://n2t.net/addgene:139054 ; RRID:Addgene_139054) |
| 参考文献部分: | A PEROXO-Tag enables rapid isolation of peroxisomes from human cells. Ray GJ, Boydston EA, Shortt E, Wyant GA, Lourido S, Chen WW, Sabatini DM. iScience, ISSN: 2589-0042, Vol: 0, Issue: 0, Page: 101109 10.1016/j.isci.2020.101109 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLJC5-3XHA-EGFP-PEX26 9076 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION HA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag..
ACCESSION .
VERSION .
KEYWORDS pLJC5-3XHA-EGFP-PEX26
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9076)
AUTHORS Ray GJ, Boydston EA, Shortt E, Wyant GA, Lourido S, Chen WW,
Sabatini DM
TITLE A PEROXO-Tag enables rapid isolation of peroxisomes from human cells
JOURNAL iScience, ISSN: 2589-0042, Vol: 0, Issue: 0, Page: 101109
REFERENCE 2 (bases 1 to 9076)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9076
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..227
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 228..408
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 455..580
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1073..1306
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1491..1535
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
promoter 1824..3035
/label=UbC promoter
/note="human ubiquitin C promoter"
primer_bind 2872..2891
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
CDS 3073..3099
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3112..3138
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3151..3177
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3190..3903
/codon_start=1
/product="enhanced GFP with monomerizing A206K mutation
(Zacharias et al., 2002)"
/label=mEGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(3232..3253)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3493..3512)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3840..3861
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 4170..4287
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 4336..4846
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 4765..4783
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 4868..5467
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4868..4887)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5364..5384
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 5595..5828
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5900..6021
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5937..5956)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5991..6010
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 6061..6196
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6123..6142
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(6216..6235)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6217..6235)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6245..6262)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6245..6261)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6254..6276)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6403..6858
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6490..6509)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6700..6721
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6885..6988
/gene="bla"
/label=AmpR promoter
CDS 6989..7849
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7207..7226)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8020..8608
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8509..8528
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8762..8779
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 8896..8917
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8932..8962
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8970..8986
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8975..8997
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 8994..9010
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8994..9010
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 9029..9049
/label=T3
/note="T3 promoter, forward primer"
promoter 9031..9049
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
901 gaccaccgca cagcaagcag cagctgatct tcagacctgg aggaggagat atgagggaca
961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
1801 gagactagcc tcgagcggga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
1861 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
1921 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
1981 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
2041 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
2101 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
2161 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
2221 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
2281 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
2341 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
2401 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
2461 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
2521 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
2581 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
2641 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
2701 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
2761 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
2821 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
2881 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
2941 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
3001 ctaaattctg gccgtttttg gcttttttgt tagacgaagc gctagcgcta ccggtttaat
3061 taagccacca tgtatcccta tgacgtgcct gattacgccg gcacaggatc ctacccctat
3121 gatgtgcctg actacgctgg cagcgccgga tacccttatg atgtgcctga ttatgctgga
3181 gggagcggcg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
3241 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
3301 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
3361 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
3421 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
3481 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
3541 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
3601 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
3661 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
3721 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
3781 aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
3841 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
3901 aagggaggga gcggacgcca gctttgggac tctgcggtga gccacttctt ttctctgccc
3961 ttcaaaaaga gtctcctggc tgccttgatc ctctgtctcc tggtggtgag atttgatcca
4021 gcttcccctt cctccctgca cttcctctac aagctggccc agctcttccg ctggatccgg
4081 aaggctgcat tttctcgcct ctaccagctc cgcatccgtg actgataaga attctcgacc
4141 tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
4201 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
4261 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
4321 tggccgcggc tcgagggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca
4381 gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg
4441 cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc
4501 cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg
4561 ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca
4621 gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg
4681 gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt
4741 gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc
4801 ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg atccaccgga
4861 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
4921 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
4981 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
5041 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
5101 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
5161 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
5221 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
5281 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
5341 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
5401 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
5461 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc
5521 atcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
5581 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
5641 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
5701 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
5761 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
5821 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
5881 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
5941 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
6001 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
6061 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
6121 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
6181 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
6241 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
6301 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
6361 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
6421 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
6481 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
6541 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
6601 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
6661 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
6721 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
6781 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
6841 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgtgcggaa cccctatttg
6901 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
6961 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
7021 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
7081 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
7141 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
7201 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
7261 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
7321 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
7381 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
7441 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
7501 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
7561 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
7621 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
7681 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
7741 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
7801 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
7861 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
7921 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
7981 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
8041 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
8101 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
8161 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
8221 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
8281 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
8341 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
8401 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
8461 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
8521 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
8581 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
8641 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
8701 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
8761 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
8821 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
8881 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
8941 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
9001 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
9061 ggagctgcaa gcttaa
//
