| 质粒名称: | dCas9-KRAB-MeCP2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 110821 |
| 存储实验室: | Alejandro Chavez, George Church |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.3_TOPO |
| 载体抗性: | 氨苄青霉素 |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | dCas9-KRAB-MeCP2,dCas9-KM |
| 物种来源: | Synthetic |
| 插入大小: | 5300 bp |
| 基因突变: | Cas9 mutations to make dCas9=D10A+D839A+H840A+N863A |
| 启动子 | CMV |
| 克隆方法: | Gibson克隆 |
| 5' 测序引物 | TCAAGTACTTCGACACCACCA |
|
文献引用方法 |
|
| 材料和方法部分: | dCas9-KRAB-MeCP2 was a gift from Alejandro Chavez & George Church (Addgene plasmid # 110821 ; http://n2t.net/addgene:110821 ; RRID:Addgene_110821) |
| 参考文献部分: | An enhanced CRISPR repressor for targeted mammalian gene regulation. Yeo NC, Chavez A, Lance-Byrne A, Chan Y, Menn D, Milanova D, Kuo CC, Guo X, Sharma S, Tung A, Cecchi RJ, Tuttle M, Pradhan S, Lim ET, Davidsohn N, Ebrahimkhani MR, Collins JJ, Lewis NE, Kiani S, Church GM. Nat Methods. 2018 Jul 16. pii: 10.1038/s41592-018-0048-5. doi: 10.1038/s41592-018-0048-5. 10.1038/s41592-018-0048-5 PubMed 30013045 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS dCas9-KRAB-MeCP2 8461 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Improved dCas9 repressor-dCas9-KRAB-MeCP2.
ACCESSION .
VERSION .
KEYWORDS dCas9-KRAB-MeCP2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8461)
AUTHORS Yeo NC, Chavez A, Lance-Byrne A, Chan Y, Menn D, Milanova D, Kuo CC,
Guo X, Sharma S, Tung A, Cecchi RJ, Tuttle M, Pradhan S, Lim ET,
Davidsohn N, Ebrahimkhani MR, Collins JJ, Lewis NE, Kiani S, Church
GM
TITLE An enhanced CRISPR repressor for targeted mammalian gene regulation.
JOURNAL Nat Methods. 2018 Jul 16. pii: 10.1038/s41592-018-0048-5. doi:
10.1038/s41592-018-0048-5.
PUBMED 30013045
REFERENCE 2 (bases 1 to 8461)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8461
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 50..429
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 430..633
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 584..604
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 630..654
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 742..751
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 748..4851
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=Cas9m4
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the mutations D10A, D839A,
H840A, and N863A (Mali et al., 2013)"
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 4864..4884
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4945..5139
/codon_start=1
/product="Kruppel-associated box (KRAB) transcriptional
repression domain from the human zinc finger protein ZNF10
(Margolin et al., 1994)"
/label=KRAB
/translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
QLTKPDVILRLEKGEEPWLV"
CDS 5173..5193
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5203..6060
/codon_start=1
/product="transcriptional repression domain from rat
methyl-CpG-binding protein 2 (Nan et al., 1997)"
/label=MeCP2
/translation="VQVKRVLEKSPGKLLVKMPFQASPGGKGEGGGATTSAQVMVIKRP
GRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRE
TVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHH
HHHAESPKAPMPLLPPPPPPEPQSSEDPISPPEPQDLSSSICKEEKMPRAGSLESDGCP
KEPAKTQPMVAAAATTTTTTTTTVAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPV
TERVS"
primer_bind complement(6115..6134)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 6159..6207
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin complement(6521..7109)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6601..6620)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7280..8140)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7903..7922
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8141..8245)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8320..8339)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
721 actctagagg atcgaaccct tgccaccatg gacaagaagt actccattgg gctcgctatc
781 ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt acaaggtgcc gagcaaaaaa
841 ttcaaagttc tgggcaatac cgatcgccac agcataaaga agaacctcat tggcgccctc
901 ctgttcgact ccggggagac ggccgaagcc acgcggctca aaagaacagc acggcgcaga
961 tatacccgca gaaagaatcg gatctgctac ctgcaggaga tctttagtaa tgagatggct
1021 aaggtggatg actctttctt ccataggctg gaggagtcct ttttggtgga ggaggataaa
1081 aagcacgagc gccacccaat ctttggcaat atcgtggacg aggtggcgta ccatgaaaag
1141 tacccaacca tatatcatct gaggaagaag cttgtagaca gtactgataa ggctgacttg
1201 cggttgatct atctcgcgct ggcgcatatg atcaaatttc ggggacactt cctcatcgag
1261 ggggacctga acccagacaa cagcgatgtc gacaaactct ttatccaact ggttcagact
1321 tacaatcagc ttttcgaaga gaacccgatc aacgcatccg gagttgacgc caaagcaatc
1381 ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca gctccctggg
1441 gagaagaaga acggcctgtt tggtaatctt atcgccctgt cactcgggct gacccccaac
1501 tttaaatcta acttcgacct ggccgaagat gccaagcttc aactgagcaa agacacctac
1561 gatgatgatc tcgacaatct gctggcccag atcggcgacc agtacgcaga cctttttttg
1621 gcggcaaaga acctgtcaga cgccattctg ctgagtgata ttctgcgagt gaacacggag
1681 atcaccaaag ctccgctgag cgctagtatg atcaagcgct atgatgagca ccaccaagac
1741 ttgactttgc tgaaggccct tgtcagacag caactgcctg agaagtacaa ggaaattttc
1801 ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg gcggagcaag ccaggaggaa
1861 ttttacaaat ttattaagcc catcttggaa aaaatggacg gcaccgagga gctgctggta
1921 aagcttaaca gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg aagcatcccc
1981 caccagattc acctgggcga actgcacgct atcctcaggc ggcaagagga tttctacccc
2041 tttttgaaag ataacaggga aaagattgag aaaatcctca catttcggat accctactat
2101 gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa atcagaagag
2161 accatcactc cctggaactt cgaggaagtc gtggataagg gggcctctgc ccagtccttc
2221 atcgaaagga tgactaactt tgataaaaat ctgcctaacg aaaaggtgct tcctaaacac
2281 tctctgctgt acgagtactt cacagtttat aacgagctca ccaaggtcaa atacgtcaca
2341 gaagggatga gaaagccagc attcctgtct ggagagcaga agaaagctat cgtggacctc
2401 ctcttcaaga cgaaccggaa agttaccgtg aaacagctca aagaagacta tttcaaaaag
2461 attgaatgtt tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa cgcatccctg
2521 ggaacgtatc acgatctcct gaaaatcatt aaagacaagg acttcctgga caatgaggag
2581 aacgaggaca ttcttgagga cattgtcctc acccttacgt tgtttgaaga tagggagatg
2641 attgaagaac gcttgaaaac ttacgctcat ctcttcgacg acaaagtcat gaaacagctc
2701 aagaggcgcc gatatacagg atgggggcgg ctgtcaagaa aactgatcaa tgggatccga
2761 gacaagcaga gtggaaagac aatcctggat tttcttaagt ccgatggatt tgccaaccgg
2821 aacttcatgc agttgatcca tgatgactct ctcaccttta aggaggacat ccagaaagca
2881 caagtttctg gccaggggga cagtcttcac gagcacatcg ctaatcttgc aggtagccca
2941 gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg atgaactcgt caaagtaatg
3001 ggaaggcata agcccgagaa tatcgttatc gagatggccc gagagaacca aactacccag
3061 aagggacaga agaacagtag ggaaaggatg aagaggattg aagagggtat aaaagaactg
3121 gggtcccaaa tccttaagga acacccagtt gaaaacaccc agcttcagaa tgagaagctc
3181 tacctgtact acctgcagaa cggcagggac atgtacgtgg atcaggaact ggacatcaat
3241 cggctctccg actacgacgt ggctgctatc gtgccccagt cttttctcaa agatgattct
3301 attgataata aagtgttgac aagatccgat aaagctagag ggaagagtga taacgtcccc
3361 tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc agctgctgaa cgccaaactg
3421 atcacacaac ggaagttcga taatctgact aaggctgaac gaggtggcct gtctgagttg
3481 gataaagccg gcttcatcaa aaggcagctt gttgagacac gccagatcac caagcacgtg
3541 gcccaaattc tcgattcacg catgaacacc aagtacgatg aaaatgacaa actgattcga
3601 gaggtgaaag ttattactct gaagtctaag ctggtctcag atttcagaaa ggactttcag
3661 ttttataagg tgagagagat caacaattac caccatgcgc atgatgccta cctgaatgca
3721 gtggtaggca ctgcacttat caaaaaatat cccaagcttg aatctgaatt tgtttacgga
3781 gactataaag tgtacgatgt taggaaaatg atcgcaaagt ctgagcagga aataggcaag
3841 gccaccgcta agtacttctt ttacagcaat attatgaatt ttttcaagac cgagattaca
3901 ctggccaatg gagagattcg gaagcgacca cttatcgaaa caaacggaga aacaggagaa
3961 atcgtgtggg acaagggtag ggatttcgcg acagtccgga aggtcctgtc catgccgcag
4021 gtgaacatcg ttaaaaagac cgaagtacag accggaggct tctccaagga aagtatcctc
4081 ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag attgggaccc caagaaatac
4141 ggcggattcg attctcctac agtcgcttac agtgtactgg ttgtggccaa agtggagaaa
4201 gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg gcatcacaat catggagcga
4261 tcaagcttcg aaaaaaaccc catcgacttt ctcgaggcga aaggatataa agaggtcaaa
4321 aaagacctca tcattaagct tcccaagtac tctctctttg agcttgaaaa cggccggaaa
4381 cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg agctggcact gccctctaaa
4441 tacgttaatt tcttgtatct ggccagccac tatgaaaagc tcaaagggtc tcccgaagat
4501 aatgagcaga agcagctgtt cgtggaacaa cacaaacact accttgatga gatcatcgag
4561 caaataagcg aattctccaa aagagtgatc ctcgccgacg ctaacctcga taaggtgctt
4621 tctgcttaca ataagcacag ggataagccc atcagggagc aggcagaaaa cattatccac
4681 ttgtttactc tgaccaactt gggcgcgcct gcagccttca agtacttcga caccaccata
4741 gacagaaagc ggtacacctc tacaaaggag gtcctggacg ccacactgat tcatcagtca
4801 attacggggc tctatgaaac aagaatcgac ctctctcagc tcggtggaga cagcagggct
4861 gaccccaaga agaagaggaa ggtgagtggt ggaggaagtg gcgggtcagg gtcgatggac
4921 gcgaaatcac ttacggcatg gtcgagaaca ctggttacgt tcaaggacgt gtttgtggac
4981 tttacacgtg aggagtggaa attgctggat actgcgcaac aaattgtgta tcgaaatgtc
5041 atgcttgaga attacaagaa cctcgtcagt ctcggatacc agttgacgaa accggatgtg
5101 atccttaggc tcgaaaaggg ggaagaacct tggctggtat cgggaggtgg ttcgggtggc
5161 tctggatcaa gcccaaagaa gaaacggaag gtggaagcct cagtgcaggt gaaaagggtg
5221 ctggaaaaat cccccggcaa actcctcgtg aagatgccct tccaggcttc ccctggcgga
5281 aaaggtgaag ggggtggcgc aaccacatct gcccaggtca tggtcatcaa gcgacctgga
5341 aggaaaagaa aggccgaggc tgaccctcag gccattccaa agaaacgggg acgcaagcca
5401 gggtccgtgg tcgcagctgc agcagctgag gctaagaaaa aggcagtgaa ggaaagctcc
5461 atccgcagtg tgcaggagac tgtcctgccc atcaagaaga ggaagactag ggagaccgtg
5521 tccatcgagg tcaaagaagt ggtcaagccc ctgctcgtgt ccaccctggg cgaaaaatct
5581 ggaaaggggc tcaaaacatg caagtcacct ggacggaaaa gcaaggagtc tagtccaaag
5641 gggcgctcaa gctccgcttc tagtccccct aaaaaggaac accatcacca tcaccatcac
5701 gccgagtctc ctaaggctcc tatgccactg ctcccaccac ctccaccacc tgagccacag
5761 tcaagcgaag accccatcag cccacccgag cctcaggatc tgtcctctag tatttgcaaa
5821 gaggaaaaga tgcccagagc aggcagcctg gagagtgatg gctgtccaaa agaacccgcc
5881 aagacccagc ctatggtggc agccgctgca actaccacca caaccacaac taccacagtg
5941 gccgaaaaat acaagcatcg cggcgagggc gaacgaaagg acattgtgtc aagctccatg
6001 cccagaccta accgggagga accagtcgat agtaggacac ccgtgactga gagagtctca
6061 tagaagaatt cgatccctac cggttagtaa tgagtttaaa cgggggaggc taactgaaac
6121 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
6181 aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
6241 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
6301 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
6361 gccctccata gtcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
6421 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
6481 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
6541 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
6601 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
6661 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
6721 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
6781 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
6841 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
6901 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
6961 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
7021 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
7081 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
7141 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
7201 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
7261 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
7321 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
7381 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
7441 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
7501 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
7561 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
7621 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
7681 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
7741 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
7801 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
7861 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
7921 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
7981 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
8041 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
8101 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
8161 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
8221 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
8281 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
8341 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
8401 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
8461 g
//
