| 出品公司: | EMD Biosciences(Novagen) |
|---|---|
| 别名: | pET42b, pet 42b, pET-42b(+) |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5929bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | N-GST, N-His, C-His |
| 载体抗性: | Kanamycin |
| 备注: |
含有GST融合蛋白;N端Thrombin蛋白酶切位点; 含有N端Factor Xa蛋白酶切位点 ; pET 42 a,b,c 的差异仅在多克隆位点区域。
|
| 产品目录号: | 70562-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET-42b(+)载体 pet42b质粒基本信息
pET-42b(+)质粒图谱 pet42b载体图谱和pET-42b(+)载体序列 pet42b质粒序列多克隆位点信息
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 120 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| S15 | 350 | 306 | |
| 6xHIS | 410 | 393 | |
| GST (variant) | 1091 | 441 | |
| T7_transl_en_RBS | 1116 | 1100 | |
| lacO | 1161 | 1134 | |
| T7_promoter | 1179 | 1161 | |
| tet (300 - 563) | 1215 | 1478 | |
| pBRrevBam_primer | 1286 | 1267 | |
| lacI | 1561 | 2652 | |
| ROP | 3224 | 3415 | |
| pGEX_3_primer | 3431 | 3409 | |
| pBR322_origin | 4449 | 3830 | |
| KanR2 | 4555 | 5370 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 3 | 1091 | 147 | |
| ORF frame 3 | 1092 | 1820 | |
| ORF frame 1 | 1693 | 2652 | |
| ORF frame 1 | 4555 | 5370 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 174 |
| NotI | 182 |
| EagI | 182 |
| HindIII | 189 |
| SalI | 195 |
| SacI | 206 |
| PstI | 214 |
| AscI | 216 |
| StuI | 226 |
| EcoRI | 235 |
| BamHI | 241 |
| EcoRV | 251 |
| NcoI | 254 |
| AgeI | 288 |
| KpnI | 295 |
| BglII | 298 |
| SacII | 389 |
| SpeI | 420 |
| MscI | 883 |
| NdeI | 1090 |
| XbaI | 1128 |
| ApaI | 2131 |
| HpaI | 2426 |
| NruI | 4643 |
| SmaI | 4860 |
| XmaI | 4858 |
pET-42b(+)质粒 pet42b载体简介
pET-42a-c系列载体是带GST蛋白标签。本载体带有N-Trx标签和His标签，S标签。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。
T7 RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。质粒的F1复制子是被定向的，所以在T7噬菌体聚合酶的作用下，包含有蛋白编码序列的病毒 粒子能够产生，并启动蛋白表达，同时蛋白表达将被T7终止子序列(Cat. No. 69337-3)的作用下终止蛋白翻译。
T7 RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。质粒的F1复制子是被定向的，所以在T7噬菌体聚合酶的作用下，包含有蛋白编码序列的病毒 粒子能够产生，并启动蛋白表达，同时蛋白表达将被T7终止子序列(Cat. No. 69337-3)的作用下终止蛋白翻译。
pET-42b(+)质粒序列 pet42b载体序列
LOCUS pET-42b(+) 5929 bp DNA circular SYN DEFINITION pET-42b(+), pet42b ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT VNTAUTHORNAME|Biofeng.com| COMMENT VNTOAUTHORAD1|Novagen Inc.| FEATURES Location/Qualifiers source 1..5929 /organism="pET-42 b(+)" /mol_type="other DNA" terminator complement(1..120) /label="T7_terminator" misc_feature 69..87 /label="T7_Terminal_primer" CDS complement(147..1091) /label="ORF frame 3" misc_feature complement(306..350) /label="S15" misc_feature complement(393..410) /label="6xHIS" gene complement(441..1091) /label="GST (variant)" /gene="GST (variant)" CDS 1092..1820 /label="ORF frame 3" misc_feature complement(1100..1116) /label="T7_transl_en_RBS" misc_feature complement(1134..1161) /label="lacO" promoter complement(1161..1179) /label="T7_promoter" gene 1215..1478 /label="tet (300 - 563)" /gene="tet (300 - 563)" misc_feature complement(1267..1286) /label="pBRrevBam_primer" misc_feature 1561..2652 /label="lacI" CDS 1693..2652 /label="ORF frame 1" misc_feature 3224..3415 /label="ROP" misc_feature complement(3409..3431) /label="pGEX_3_primer" rep_origin complement(3830..4449) /label="pBR322_origin" gene 4555..5370 /label="KanR2" /gene="KanR2" CDS 4555..5370 /label="ORF frame 1" ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt 181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc 241 ggatccccga tatccatgga cccgcgtccc tcaataccgg agccaccacc ggtacccaga 301 tctgggctgt ccatgtgctg gcgttcgaat ttagcagcag cggtttcttt cataccaatt 361 gcagtactac cgcgtggcac cagacccgcg gagtgatggt gatggtgatg accagaacca 421 ctagttgaac catccgattt tggaggatgg tcgccaccac caaacgtggc ttgccagccc 481 tgcaaaggcc atgctatata cttgctggat ttcaagtact tatcaatttg tgggatagct 541 tcaatacgtt ttttaaaaca aactaatttt gggaacgcat ccaggcacat tgggtccatg 601 tataaaacaa catcaagagc gtcatacaac atgaagtcag gatgggttac atgatcacca 661 tttaaatatg ttttatgaca taaacgatct tcgaacattt tcagcatttc aggtagcttg 721 ctaagaaaat caactttgag agtttcaaag tctttactat atgcaattct cgaaacaccg 781 tatctaatat ccaaaaccgc tccttcaagc attgaaatct ctgcacgctc ttttggacaa 841 ccacccaaca tgttgtgctt gtcagctata taacgtatga tggccataga ctgtgttaat 901 ttaacatcac catcaatata ataaggaaga ttgggaaact ccaaacccaa ttcaaacttt 961 ttgtttcgcc atttatcacc ttcatcgcgc tcatacaaat gctcttcata tttttcttca 1021 agatattcca aaagaagtcg agtgggttgc acaaggccct taattttcca ataacctagt 1081 ataggggaca tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat 1141 tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatcga 1201 tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg 1261 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca 1321 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg 1381 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac 1441 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc 1501 gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg 1561 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat 1621 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa 1681 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg 1741 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg 1801 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg 1861 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa 1921 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa 1981 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac 2041 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg 2101 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt 2161 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa 2221 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc 2281 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt 2341 accgagtccg ggctgcgcgt tggtgcggac atctcggtag tgggatacga cgataccgaa 2401 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg 2461 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag 2521 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc 2581 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 2641 agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg caccgggatc 2701 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat 2761 gactagcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac 2821 tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac 2881 gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga 2941 aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg 3001 gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat 3061 ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa 3121 ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat 3181 cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa 3241 aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact 3301 caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga 3361 tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 3421 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 3481 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag 3541 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg 3601 caccatatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 3661 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 3721 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 3781 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 3841 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 3901 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 3961 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 4021 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 4081 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 4141 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 4201 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 4261 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 4321 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 4381 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 4441 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 4501 tggtcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc 4561 catattcaac gggaaacgtc ttgctctagg ccgcgattaa attccaacat ggatgctgat 4621 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga 4681 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc 4741 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg 4801 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc 4861 gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat 4921 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac 4981 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat 5041 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg 5101 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat 5161 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc 5221 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca 5281 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag 5341 tttcatttga tgctcgatga gtttttctaa gaattaattc atgagcggat acatatttga 5401 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 5461 tgaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 5521 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 5581 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 5641 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 5701 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 5761 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 5821 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 5881 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgcca //
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pET-42b(+)质粒 pet42b载体应用举例
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