| 出品公司: | -- |
|---|---|
| 载体名称: | pset152 |
| 质粒类型: | 链霉菌整合载体，链霉菌基因过表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无启动子，需要自己加入相应启动子 |
| 载体大小: | 5.7 kb |
| 5' 测序引物及序列: | M13 (-21) Forward：TGTAAAACGACGGCCAGT 或者M13 (-40) Forword：GTTTTCCCAGTCACGAC |
| 3' 测序引物及序列: | M13-R:CAGGAAACAGCTATGAC |
| 载体标签: | -- |
| 载体抗性: | 安普霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 链霉菌，放线菌等 |
| 备注: | pSET'152载体能够将外源基因整合进入链霉菌基因组中，是链霉菌基因组高表达特定基因时常用的基因导入载体。 |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pSET152载体 pSET152链霉菌整合质粒基本信息
pSET152质粒图谱 pSET152链霉菌整合载体图谱和pSET152载体序列 pSET152链霉菌整合质粒序列多克隆位点信息
pSET152质粒 pSET152链霉菌整合载体简介
pSET152质粒序列 pSET152链霉菌整合载体序列
LOCUS pSET152 5715 bp DNA circular SYN 14-NOV-2006
DEFINITION Cloning vector PSET152.
ACCESSION AJ414670
VERSION AJ414670.1 GI:17974209
KEYWORDS aac(3)IV gene; apramycin resistance gene; int gene; integrase; lacZ
alpha peptide; lacZa gene.
SOURCE Cloning vector pSET152
ORGANISM Cloning vector pSET152
other sequences; artificial sequences; vectors.
REFERENCE 1
AUTHORS Wilkinson,C.J., Hughes-Thomas,Z.A., Martin,C.J., Bohm,I.,
Mironenko,T., Deacon,M., Wheatcroft,M., Wirtz,G., Staunton,J. and
Leadlay,P.F.
TITLE Increasing the efficiency of heterologous promoters in
actinomycetes
JOURNAL J. Mol. Microbiol. Biotechnol. 4 (4), 417-426 (2002)
PUBMED 12125822
REFERENCE 2 (bases 1 to 5715)
AUTHORS Wilkinson,C.J.
TITLE Direct Submission
JOURNAL Submitted (28-SEP-2001) Wilkinson C.J., Department of Biochemistry,
University of Cambridge, 80 Tennis Court Rd., Cambridge, CB2 1GA,
UNITED KINGDOM
COMMENT Related sequences V00635, J02451, M24524, M21778.
FEATURES Location/Qualifiers
source 1..5715
/organism="Cloning vector pSET152"
/mol_type="other DNA"
/host="Streptomyces sp."
/db_xref="taxon:173260"
/lab_host="Escherichia coli"
gene complement(163..507)
/gene="lacZa"
CDS complement(163..507)
/gene="lacZa"
/function="blue-white screening"
/codon_start=1
/transl_table=11
/product="lacZ alpha peptide"
/protein_id="CAC93946.1"
/db_xref="GI:17974210"
variation complement(508..509)
/label="pUC18"
/replace="ggt"
gene 1729..2505
/gene="aac(3)IV"
CDS 1729..2505
/gene="aac(3)IV"
/function="apramycin resistance"
/codon_start=1
/transl_table=11
/product="apramycin resistance gene"
/protein_id="CAC93947.1"
/db_xref="GI:17974211"
rep_origin 3340..3451
/label="RP4 origin of single-stranded DNA transfer"
/direction=RIGHT
misc_feature 3799..3837
/label="phiC31 attP site"
gene 3854..5695
/gene="int"
CDS 3854..5695
/gene="int"
/function="phiC31 integrase"
/codon_start=1
/transl_table=11
/product="integrase"
/protein_id="CAC93948.1"
/db_xref="GI:17974212"
ORIGIN
1 atctacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
61 gctctcgcag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
121 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttg atcggcactt
181 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattta tgcggtgtga
241 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
301 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
361 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
421 ttgtaaaacg acggccagtg ccaagcttgg gctgcaggtc gactctagag gatccgcggc
481 cgcgcgcgat atcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
541 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
601 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
661 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
721 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
781 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
841 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
901 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
961 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
1021 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
1081 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
1141 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
1201 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
1261 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
1321 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
1381 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
1441 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
1501 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
1561 gattttggtc atgagattat caaaaaggat cttcacctag atccttttgg ttcatgtgca
1621 gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt
1681 tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa
1741 tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg
1801 ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg
1861 atcgaggccc tgcgtgctgc gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca
1921 ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt
1981 gtctctgaca cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt
2041 gcggcagcgg ggccacaggc agagcagatc atctctgatc cattgcccct gccacctcac
2101 tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc
2161 gtgggacacg atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat
2221 ggggtgccga gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc
2281 gagaatgacc actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc
2341 cttcagaagg aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt
2401 gtggcgacag ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag
2461 gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga
2521 acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg tggagcggat
2581 cggggattgt ctttcttcag ctcgctgatg atatgctgac gctcaatgcc gtttggcctc
2641 cgactaacga aaatcccgca tttggacggc tgatccgatt ggcacggcgg acggcgaatg
2701 gcggagcaga cgctcgtccg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
2761 tatcgggccc tggccagcta gctagagtcg acctgcaggt ccccggggat cggtcttgcc
2821 ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg ctcttcttga tggagcgcat
2881 ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg
2941 gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc tgcttctctt
3001 cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt
3061 cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg atgcgggcca
3121 gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg ccgacggcca
3181 gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt
3241 cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc ttggtttcat
3301 cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag
3361 gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg
3421 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt
3481 ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa
3541 aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagatccgt cgacctgcag
3601 gcatgcaagc tctagcgatt ccagacgtcc cgaaggcgtg gcgcggcttc cccgtgccgg
3661 agcaatcgcc ctgggtgggt tacacgacgc ccctctatgg cccgtactga cggacacacc
3721 gaagccccgg cggcaaccct cagcggatgc cccggggctt cacgttttcc caggtcagaa
3781 gcggttttcg ggagtagtgc cccaactggg gtaacctttg agttctctca gttgggggcg
3841 tagggtcgcc gacatgacac aaggggttgt gaccggggtg gacacgtacg cgggtgctta
3901 cgaccgtcag tcgcgcgagc gcgagaattc gagcgcagca agcccagcga cacagcgtag
3961 cgccaacgaa gacaaggcgg ccgaccttca gcgcgaagtc gagcgcgacg ggggccggtt
4021 caggttcgtc gggcatttca gcgaagcgcc gggcacgtcg gcgttcggga cggcggagcg
4081 cccggagttc gaacgcatcc tgaacgaatg ccgcgccggg cggctcaaca tgatcattgt
4141 ctatgacgtg tcgcgcttct cgcgcctgaa ggtcatggac gcgattccga ttgtctcgga
4201 attgctcgcc ctgggcgtga cgattgtttc cactcaggaa ggcgtcttcc ggcagggaaa
4261 cgtcatggac ctgattcacc tgattatgcg gctcgacgcg tcgcacaaag aatcttcgct
4321 gaagtcggcg aagattctcg acacgaagaa ccttcagcgc gaattgggcg ggtacgtcgg
4381 cgggaaggcg ccttacggct tcgagcttgt ttcggagacg aaggagatca cgcgcaacgg
4441 ccgaatggtc aatgtcgtca tcaacaagct tgcgcactcg accactcccc ttaccggacc
4501 cttcgagttc gagcccgacg taatccggtg gtggtggcgt gagatcaaga cgcacaaaca
4561 ccttcccttc aagccgggca gtcaagccgc cattcacccg ggcagcatca cggggctttg
4621 taagcgcatg gacgctgacg ccgtgccgac ccggggcgag acgattggga agaagaccgc
4681 ttcaagcgcc tgggacccgg caaccgttat gcgaatcctt cgggacccgc gtattgcggg
4741 cttcgccgct gaggtgatct acaagaagaa gccggacggc acgccgacca cgaagattga
4801 gggttaccgc attcagcgcg acccgatcac gctccggccg gtcgagcttg attgcggacc
4861 gatcatcgag cccgctgagt ggtatgagct tcaggcgtgg ttggacggca gggggcgcgg
4921 caaggggctt tcccgggggc aagccattct gtccgccatg gacaagctgt actgcgagtg
4981 tggcgccgtc atgacttcga agcgcgggga agaatcgatc aaggactctt accgctgccg
5041 tcgccggaag gtggtcgacc cgtccgcacc tgggcagcac gaaggcacgt gcaacgtcag
5101 catggcggca ctcgacaagt tcgttgcgga acgcatcttc aacaagatca ggcacgccga
5161 aggcgacgaa gagacgttgg cgcttctgtg ggaagccgcc cgacgcttcg gcaagctcac
5221 tgaggcgcct gagaagagcg gcgaacgggc gaaccttgtt gcggagcgcg ccgacgccct
5281 gaacgccctt gaagagctgt acgaagaccg cgcggcaggc gcgtacgacg gacccgttgg
5341 caggaagcac ttccggaagc aacaggcagc gctgacgctc cggcagcaag gggcggaaga
5401 gcggcttgcc gaacttgaag ccgccgaagc cccgaagctt ccccttgacc aatggttccc
5461 cgaagacgcc gacgctgacc cgaccggccc taagtcgtgg tgggggcgcg cgtcagtaga
5521 cgacaagcgc gtgttcgtcg ggctcttcgt agacaagatc gttgtcacga agtcgactac
5581 gggcaggggg cagggaacgc ccatcgagaa gcgcgcttcg atcacgtggg cgaagccgcc
5641 gaccgacgac gacgaagacg acgcccagga cggcacggaa gacgtagcgg cgtagcgaga
5701 cacccgggaa gcctg
//
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pSET152质粒 pSET152链霉菌整合载体应用举例
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