| 质粒名称: | pMSCV-hygro-STING |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 102598 |
| 存储实验室: | Nicolas Manel |
|
载体骨架基本信息 |
|
| 载体名称: | pMSCV-hygro |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | STING |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | MSCV |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMSCV-hygro-STING was a gift from Nicolas Manel (Addgene plasmid # 102598 ; http://n2t.net/addgene:102598 ; RRID:Addgene_102598) |
| 参考文献部分: | Intrinsic antiproliferative activity of the innate sensor STING in T lymphocytes. Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg MC, Picard C, Neven B, Fischer A, Amigorena S, Rieux-Laucat F, Manel N. J Exp Med. 2017 Jun 5;214(6):1769-1785. doi: 10.1084/jem.20161674. Epub 2017 May 8. 10.1084/jem.20161674 PubMed 28484079 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMSCV-hygro-STING 8118 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Express STING for IFN-beta luciferase reporter assay..
ACCESSION .
VERSION .
KEYWORDS pMSCV-hygro-STING
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8118)
AUTHORS Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg
MC, Picard C, Neven B, Fischer A, Amigorena S, Rieux-Laucat F, Manel
N
TITLE Intrinsic antiproliferative activity of the innate sensor STING in T
lymphocytes.
JOURNAL J Exp Med. 2017 Jun 5;214(6):1769-1785. doi: 10.1084/jem.20161674.
Epub 2017 May 8.
PUBMED 28484079
REFERENCE 2 (bases 1 to 8118)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8118
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 579..920
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 987..1403
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1333..1355
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1371..1387
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
promoter 2578..3077
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(2613..2636)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 3010..3029
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 3095..4120
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
LTR 4478..4992
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 5187..5203
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5211..5241)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5256..5277
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5394..5411)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5565..6153)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5645..5664)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6324..7184)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6947..6966
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7185..7289)
/gene="bla"
/label=AmpR promoter
primer_bind 7357..7375
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(7413..7435)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 7535..7554
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7748..7770
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 7898..7917
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
LTR 8117..515
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
ORIGIN
1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
1381 ccctcactcc ttctctaggc gccggaatta gatccatgcc ccactccagc ctgcatccat
1441 ccatcccgtg tcccaggggt cacggggccc agaaggcagc cttggttctg ctgagtgcct
1501 gcctggtgac cctttggggg ctaggagagc caccagagca cactctccgg tacctggtcc
1561 tccacctagc ctccctgcag ctgggactgc tgttaaacgg ggtctgcagc ctggctgagg
1621 agctgcgcca catccactcc aggtaccggg gcagctactg gaggactgtg cgggcctgcc
1681 tgggctgccc cctccgccgt ggggccctgt tgctgctgtc catctatttc tactactccc
1741 tcccaaatgc ggtcggcccg cccttcactt ggatgcttgc cctcctgggc ctctcgcagg
1801 cactgaacat cctcctgggc ctcaagggcc tggccccagc tgagatctct gcagtgtgtg
1861 aaaaagggaa tttcaacgtg gcccatgggc tggcatggtc atattacatc ggatatctgc
1921 ggctgatcct gccagagctc caggcccgga ttcgaactta caatcagcat tacaacaacc
1981 tgctacgggg tgcagtgagc cagcggctgt atattctcct cccattggac tgtggggtgc
2041 ctgataacct gagtatggct gaccccaaca ttcgcttcct ggataaactg ccccagcaga
2101 ccggtgaccg tgctggcatc aaggatcggg tttacagcaa cagcatctat gagcttctgg
2161 agaacgggca gcgggcgggc acctgtgtcc tggagtacgc cacccccttg cagactttgt
2221 ttgccatgtc acaatacagt caagctggct ttagccggga ggataggctt gagcaggcca
2281 aactcttctg ccggacactt gaggacatcc tggcagatgc ccctgagtct cagaacaact
2341 gccgcctcat tgcctaccag gaacctgcag atgacagcag cttctcgctg tcccaggagg
2401 ttctccggca cctgcggcag gaggaaaagg aagaggttac tgtgggcagc ttgaagacct
2461 cagcggtgcc cagtacctcc acgatgtccc aagagcctga gctcctcatc agtggaatgg
2521 aaaagcccct ccctctccgc acggatttct cttgactcga ggttaacgaa ttctaccggg
2581 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
2641 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
2701 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
2761 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
2821 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
2881 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
2941 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
3001 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
3061 catctccggg cctttcgacc tgcatcccgc caccatgaaa aagcctgaac tcaccgcgac
3121 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
3181 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
3241 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
3301 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
3361 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
3421 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
3481 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
3541 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
3601 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
3661 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
3721 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
3781 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
3841 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
3901 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
3961 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
4021 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
4081 aaaccgacgc cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgaacaa
4141 gagctgattt cgagaacgcc tcagccagca actcgcgcga gcctagcaag gcaaatgcga
4201 gagaacggcc ttacgcttgg tggcacagtt ctcgtccaca gttcgctaag ctcgctcggc
4261 tgggtcgcgg gagggccggt cgcagtgatt caggcccttc tggattgtgt tggtccccag
4321 ggcacgattg tcatgcccac gcactcgggt gatctgactg atcccgcaga ttggagatcg
4381 ccgcccgtgc ctgccgattg ggtgcagatc cgtcgacctg cagccaagct tatcgataaa
4441 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
4501 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaatacata actgagaata
4561 gagaagttca gatcaaggtt aggaacagag agacagcaga atatgggcca aacaggatat
4621 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
4681 cccgccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
4741 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
4801 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc
4861 cgatagactg cgtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
4921 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
4981 ggggtctttc atgggtaaca gtttcttgaa gttggagaac aacattctga gggtaggagt
5041 cgaatattaa gtaatcctga ctcaattagc cactgttttg aatccacata ctccaatact
5101 cctgaaatag ttcattatgg acagcgcaga aagagctgga kagttcatta tggacagcgc
5161 agaaagagct ggggagaatt gtgaaattgt tatccgctca caattccaca caacatacga
5221 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
5281 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
5341 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
5401 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
5461 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
5521 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
5581 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
5641 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
5701 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
5761 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
5821 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
5881 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
5941 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
6001 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
6061 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
6121 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
6181 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
6241 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
6301 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
6361 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
6421 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
6481 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
6541 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
6601 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
6661 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
6721 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
6781 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
6841 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
6901 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
6961 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
7021 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
7081 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
7141 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
7201 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
7261 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
7321 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
7381 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
7441 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
7501 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
7561 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
7621 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
7681 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
7741 gggttttccc agtcacgacg ttgtaaaacg acggcgcaag gaatggtgca tgcaaggaga
7801 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
7861 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
7921 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggcgat
7981 tagtccaatt tgttaaagac aggatatcag tggtccaggc tctagttttg actcaacaat
8041 atcaccagct gaagcctata gagtacgagc catagataaa ataaaagatt ttatttagtc
8101 tccagaaaaa ggggggaa
//
