| 质粒名称: | GFP_CD47_LU |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 65474 |
| 存储实验室: | Christine Mayr |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5372 bp |
| 完整质粒大小: | 11262 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Signal peptide of CD47 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 60 bp |
| 基因突变: | -- |
| GenBank编号 | NM_001777.3 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | BamHI（销毁后未知） |
| 5' 测序引物 | T7 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | GFP_CD47_LU was a gift from Christine Mayr (Addgene plasmid # 65474 ; http://n2t.net/addgene:65474 ; RRID:Addgene_65474) |
| 参考文献部分: | Alternative 3' UTRs act as scaffolds to regulate membrane protein localization. Berkovits BD, Mayr C. Nature. 2015 Apr 20. doi: 10.1038/nature14321. 10.1038/nature14321 PubMed 25896326 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS GFP_CD47_LU 11265 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION GFP tagged CD47 with its own long 3'UTR.
ACCESSION .
VERSION .
KEYWORDS GFP_CD47_LU
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11265)
AUTHORS Berkovits BD, Mayr C
TITLE Alternative 3' UTRs act as scaffolds to regulate membrane protein
localization.
JOURNAL Nature. 2015 Apr 20. doi: 10.1038/nature14321.
PUBMED 25896326
REFERENCE 2 (bases 1 to 11265)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11265
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 995..1711
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1040..1061)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1301..1320)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1648..1669
/label=EGFP-C
/note="EGFP, forward primer"
RBS 3406..3417
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
primer_bind complement(6859..6876)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6865..7089
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 7135..7563
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7222..7241)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7432..7453
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7572..7592)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7577..7906
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7757..7892
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7819..7838
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 7973..8767
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(8027..8046)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 8637..8656
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 8941..9062
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8978..8997)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9032..9051
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(9111..9127)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9111..9127)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9124..9146)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9135..9151
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9159..9189)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9204..9225
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9342..9359)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9513..10098)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9593..9612)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10269..11129)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10892..10911
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11130..11234)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccatgtgg cccctggtag cggcgctgtt
961 gctgggctcg gcgtgctgcg gatcagctgg atccatggtg agcaagggcg aggagctgtt
1021 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
1081 cgtgtctggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
1141 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
1201 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
1261 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
1321 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
1381 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
1441 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg
1501 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
1561 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
1621 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
1681 gatcactctc ggcatggacg agctgtacaa gcagctacta tttaataaaa caaaatctgt
1741 agaattcacg ttttgtaatg acactgtcgt cattccatgc tttgttacta atatggaggc
1801 acaaaacact actgaagtat acgtaaagtg gaaatttaaa ggaagagata tttacacctt
1861 tgatggagct ctaaacaagt ccactgtccc cactgacttt agtagtgcaa aaattgaagt
1921 ctcacaatta ctaaaaggag atgcctcttt gaagatggat aagagtgatg ctgtctcaca
1981 cacaggaaac tacacttgtg aagtaacaga attaaccaga gaaggtgaaa cgatcatcga
2041 gctaaaatat cgtgttgttt catggttttc tccaaatgaa aatattctta ttgttatttt
2101 cccaattttt gctatactcc tgttctgggg acagtttggt attaaaacac ttaaatatag
2161 atccggtggt atggatgaga aaacaattgc tttacttgtt gctggactag tgatcactgt
2221 cattgtcatt gttggagcca ttcttttcgt cccaggtgaa tattcattaa agaatgctac
2281 tggccttggt ttaattgtga cttctacagg gatattaata ttacttcact actatgtgtt
2341 tagtacagcg attggattaa cctccttcgt cattgccata ttggttattc aggtgatagc
2401 ctatatcctc gctgtggttg gactgagtct ctgtattgcg gcgtgtatac caatgcatgg
2461 ccctcttctg atttcaggtt tgagtatctt agctctagca caattacttg gactagttta
2521 tatgaaattt gtggcttcca atcagaagac tatacaacct cctaggaaag ctgtagagga
2581 accccttaat gcattcaaag aatcaaaagg aatgatgaat gatgaataag cggccgcagt
2641 gaagtgatgg actccgattt ggagagtagt aagacgtgaa aggaatacac ttgtgtttaa
2701 gcaccatggc cttgatgatt cactgttggg gagaagaaac aagaaaagta actggttgtc
2761 acctatgaga cccttacgtg attgttagtt aagtttttat tcaaagcagc tgtaatttag
2821 ttactcaaat aattatgatc tatgttgttt gcccaattga gatccagttt tttgttgtta
2881 tttttaatca attaggggca atagtagaat ggacaatttc caagaatgat gcctttcagg
2941 tcctagggcc tctggcctct aggtaaccag tttaaattgg ttcagggtga taactactta
3001 gcactgccct ggtgattacc cagagatatc tatgaaaacc agtggcttcc atcaaacctt
3061 tgccaactca ggttcacagc agctttgggc agttatggca gtatggcatt agctgagagg
3121 tgtctgccac ttctgggtca atggaataat aaattaagta caggcaggaa tttggttggg
3181 agcatcttgt atgatctccg tatgatgtga tattgatgga gatagtggtc ctcattcttg
3241 ggggttgcca ttcccacatt cccccttcaa caaacagtgt aacaggtcct tcccagattt
3301 agggtacttt tattgatgga tatgttttcc ttttattcac ataacccctt gaaaccctgt
3361 cttgtcctcc tgttacttgc ttctgctgta caagatgtag caccttttct cctctttgaa
3421 catggtctag tgacacggta gcaccagttg caggaaggag ccagacttgt tctcagagca
3481 ctgtgttcac acttttcagc aaaaatagct atggttgtaa catatgtatt cccttcctct
3541 gatttgaagg caaaaatcta cagtgtttct tcacttcttt tctgatctgg ggcatgaaaa
3601 aagcaagatt gaaatttgaa ctatgagtct cctgcatggc aacaaaatgt gtgtcaccat
3661 caggccaaca ggccagccct tgaatgggga tttattactg ttgtatctat gttgcatgat
3721 aaacattcat caccttcctc ctgtagtcct gcctcgtact ccccttcccc tatgattgaa
3781 aagtaaacaa aacccacatt tcctatcctg gttagaagaa aattaatgtt ctgacagttg
3841 tgatcgcctg gagtactttt agacttttag cattcgtttt ttacctgttt gtggatgtgt
3901 gtttgtatgt gcatacgtat gagataggca catgcatctt ctgtatggac aaaggtgggg
3961 tacctacagg agagcaaagg ttaattttgt gcttttagta aaaacattta aatacaaagt
4021 tctttattgg gtggaattat atttgatgca aatatttgat cacttaaaac ttttaaaact
4081 tctaggtaat ttgccacgct ttttgactgc tcaccaatac cctgtaaaaa tacgtaattc
4141 ttcctgtttg tgtaataaga tattcatatt tgtagttgca ttaataatag ttatttctta
4201 gtccatcaga tgttcccgtg tgcctctttt atgccaaatt gattgtcata tttcatgttg
4261 ggaccaagta gtttgcccat ggcaaaccta aatttatgac ctgctgaggc ctctcagaaa
4321 actgagcata ctagcaagac agctcttctt gaaaaaaaaa atatgtatac acaaatatat
4381 acgtatatct atatatacgt atgtatatac acacatgtat attcttcctt gattgtgtag
4441 ctgtccaaaa taataacata tatagaggga gctgtattcc tttatacaaa tctgatggct
4501 cctgcagcac tttttccttc tgaaaatatt tacattttgc taacctagtt tgttacttta
4561 aaaatcagtt ttgatgaaag gagggaaaag cagatggact tgaaaaagat ccaagctcct
4621 attagaaaag gtatgaaaat ctttatagta aaatttttta taaactaaag ttgtaccttt
4681 taatatgtag taaactctca tttatttggg gttcgctctt ggatctcatc catccattgt
4741 gttctcttta atgctgcctg ccttttgagg cattcactgc cctagacaat gccaccagag
4801 atagtggggg aaatgccaga tgaaaccaac tcttgctctc actagttgtc agcttctctg
4861 gataagtgac cacagaagca ggagtcctcc tgcttgggca tcattgggcc agttccttct
4921 ctttaaatca gatttgtaat ggctcccaaa ttccatcaca tcacatttaa attgcagaca
4981 gtgttttgca catcatgtat ctgttttgtc ccataatatg ctttttactc cctgatccca
5041 gtttctgctg ttgactcttc cattcagttt tatttattgt gtgttctcac agtgacacca
5101 tttgtccttt tctgcaacaa cctttccagc tacttttgcc aaattctatt tgtcttctcc
5161 ttcaaaacat tctcctttgc agttcctctt catctgtgta gctgctcttt tgtctcttaa
5221 cttaccattc ctatagtact ttatgcatct ctgcttagtt ctattagttt tttggccttg
5281 ctcttctcct tgattttaaa attccttcta tagctagagc ttttctttct ttcattctct
5341 cttcctgcag tgttttgcat acatcagaag ctaggtacat aagttaaatg attgagagtt
5401 ggctgtattt agatttatca ctttttaata gggtgagctt gagagttttc tttctttctg
5461 tttttttttt ttgttttttt tttttttttt tttttttttt tttttgacta atttcacatg
5521 ctctaaaaac cttcaaaggt gattattttt ctcctggaaa ctccaggtcc attctgttta
5581 aatccctaag aatgtcagaa ttaaaataac agggctatcc cgtaattgga aatatttctt
5641 ttttcaggat gctatagtca atttagtaag tgaccaccaa attgttattt gcactaacaa
5701 agctcaaaac acgataagtt tactcctcca tctcagtaat aaaaattaag ctgtaatcaa
5761 ccttctaggt ttctcttgtc ttaaaatggg tattcaaaaa tggggatctg tggtgtatgt
5821 atggaaacac atactcctta atttacctgt tgttggaaac tggagaaatg attgtcgggc
5881 aaccgtttat tttttattgt attttatttg gttgagggat ttttttataa acagttttac
5941 ttgtgtcata ttttaaaatt actaactgcc atcacctgct ggggtccttt gttaggtcat
6001 tttcagtgac taatagggat aatccaggta actttgaaga gatgagcagt gagtgaccag
6061 gcagtttttc tgcctttagc tttgacagtt cttaattaag atcattgaag accagctttc
6121 tcataaattt ctctttttga aaaaaagaaa gcatttgtac taagctcctc tgtaagacaa
6181 catcttaaat cttaaaagtg ttgttatcat gactggtgag agaagaaaac attttgtttt
6241 tattaaatgg agcattattt acaaaaagcc attgttgaga attagatccc acatcgtata
6301 aatatctatt aaccattcta aataaagaga actccagtgt tgctatgtgc aagatcctct
6361 cttggagctt ttttgcatag caattaaagg tgtgctattt gtcagtagcc atttttttgc
6421 agtgatttga agaccaaagt tgttttacag ctgtgttacc gttaaaggtt ttttttttta
6481 tatgtattaa atcaatttat cactgtttaa agctttgaat atctgcaatc tttgccaagg
6541 tactttttta tttaaaaaaa aacataactt tgtaaatatt accctgtaat attatatata
6601 cttaataaaa cattttaagc tattttgttg ggctatttct attgctgcta cagcagacca
6661 caagcacatt tctgaaaaat ttaatttatt aatgtatttt taagttgctt atattctagg
6721 taacaatgta aagaatgatt taaaatatta attatgaatt ttttgagtat aatacccaat
6781 aagcttttaa ttagagcaga gttttaatta aaagttttaa atcagtctct agagggcccg
6841 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
6901 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
6961 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
7021 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
7081 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
7141 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
7201 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
7261 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
7321 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
7381 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
7441 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
7501 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
7561 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
7621 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
7681 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
7741 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
7801 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
7861 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
7921 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
7981 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
8041 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
8101 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
8161 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
8221 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
8281 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
8341 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
8401 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
8461 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
8521 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
8581 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
8641 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
8701 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
8761 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
8821 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
8881 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
8941 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
9001 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
9061 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
9121 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
9181 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
9241 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
9301 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
9361 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
9421 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
9481 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
9541 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
9601 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
9661 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
9721 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
9781 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
9841 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
9901 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
9961 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
10021 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc
10081 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
10141 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
10201 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
10261 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
10321 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
10381 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
10441 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
10501 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
10561 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
10621 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
10681 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
10741 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
10801 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
10861 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
10921 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
10981 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
11041 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
11101 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
11161 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
11221 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
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