| 出品人: | Fodor,B.D.(2004) |
|---|---|
| 载体名称: | pMHE3Tc |
| 质粒类型: | 广宿主载体；细菌表达载体;体外表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | T7/lac |
| 载体大小: | 6384 bp |
| 5' 测序引物及序列: | T7 promoter 5’TAATACGACTCACTATAGGG 3’ |
| 3' 测序引物及序列: | T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’ |
| 载体标签: | T7 tag（N-ter）；6xHis tag (C-ter) |
| 载体抗性: | 四环素 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 多个菌属 |
| 备注: | -- |
| 产品目录号: | -- |
| 组成型/诱导型: | 诱导型 |
买家导航
pMHE3Tc载体质粒基本信息
pMHE3Tc质粒图谱载体图谱和pMHE3Tc载体序列质粒序列多克隆位点信息
pMHE3Tc质粒载体简介
pMHE3Tc质粒序列载体序列
LOCUS AY299695 6384 bp DNA circular SYN 10-FEB-2004
DEFINITION Broad host range expression vector pMHE3Tc, complete sequence.
ACCESSION AY299695
VERSION AY299695.1 GI:34334144
AUTHORS Fodor,B.D., Kovacs,A.T., Csaki,R., Hunyadi-Gulyas,E., Klement,E.,
Maroti,G., Meszaros,L.S., Medzihradszky,K.F., Rakhely,G. and
Kovacs,K.L.
TITLE Modular broad-host-range expression vectors for single-protein and
protein complex purification
JOURNAL Appl. Environ. Microbiol. 70 (2), 712-721 (2004)
PUBMED 14766546
JOURNAL Submitted (16-MAY-2003) Biophysics, BRC Hungarian Academy of
Sciences, Temesvari krt., Szeged, Csongrad 6701, Hungary
FEATURES Location/Qualifiers
source 1..6384
/organism="Broad host range expression vector pMHE3Tc"
/mol_type="genomic DNA"
/db_xref="taxon:244053"
primer_bind 198..216
/note="T7 promoter primer"
regulatory 198..214
/regulatory_class="promoter"
/note="T7 promoter"
misc_feature 215..240
/note="lac operator"
regulatory 271..277
/regulatory_class="ribosome_binding_site"
misc_feature 286..318
/note="T7-tag"
misc_feature 367..384
/note="His-tag"
primer_bind complement(437..454)
/note="T7 terminator primer"
regulatory 450..497
/regulatory_class="terminator"
/note="T7 terminator"
rep_origin 1523..2185
/note="pBBR1 oriV"
misc_feature 3179..4172
/note="RP4 mob; oriT"
regulatory complement(4262..4377)
/regulatory_class="terminator"
/note="T4 terminator"
gene complement(4683..5873)
/gene="tet"
CDS complement(4683..5873)
/gene="tet"
/codon_start=1
/transl_table=11
/product="tetracycline resistance protein"
/protein_id="AAQ64666.1"
/db_xref="GI:34334145"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
regulatory 6258..6373
/regulatory_class="terminator"
/note="T4 terminator"
ORIGIN
1 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
61 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
121 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
181 tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc
241 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatggc tagcatgact
301 ggtggacagc aaatgggtcg ggatccgaat tcgagctccg tcgacaagct tgcggccgca
361 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
421 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
481 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
541 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
601 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
661 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
721 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
781 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
841 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
901 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
961 attttaacaa aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
1021 taaccaatag gccgactgcg atgagtggca gggcggggcg taattttttt aaggcagtta
1081 ttggtgccct taaacgcctg gtgctacgcc tgaataagtg ataataagcg gatgaatggc
1141 agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg
1201 cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct
1261 tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata
1321 tgatcattta ttctgcctcc cagagcctga taaaaacggt gaatccgtta gcgaggtgcc
1381 gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag
1441 gcagacaagg tatagggcgg cgaggcggct acagccgata gtctggaaca gcgcacttac
1501 gggttgctgc gcaacccaag tgctaccggc gcggcagcgt gacccgtgtc ggcggctcca
1561 acggctcgcc atcgtccaga aaacacggct catcgggcat cggcaggcgc tgctgcccgc
1621 gccgttccca ttcctccgtt tcggtcaagg ctggcaggtc tggttccatg cccggaatgc
1681 cgggctggct gggcggctcc tcgccggggc cggtcggtag ttgctgctcg cccggataca
1741 gggtcgggat gcggcgcagg tcgccatgcc ccaacagcga ttcgtcctgg tcgtcgtgat
1801 caaccaccac ggcggcactg aacaccgaca ggcgcaactg gtcgcggggc tggccccacg
1861 ccacgcggtc attgaccacg taggccgaca cggtgccggg gccgttgagc ttcacgacgg
1921 agatccagcg ctcggccacc aagtccttga ctgcgtattg gaccgtccgc aaagaacgtc
1981 cgatgagctt ggaaagtgtc ttctggctga ccaccacggc gttctggtgg cccatctgcg
2041 ccacgaggtg atgcagcagc attgccgccg tgggtttcct cgcaataagc ccggcccacg
2101 cctcatgcgc tttgcgttcc gtttgcaccc agtgaccggg cttgttcttg gcttgaatgc
2161 cgatttctct ggactgcgtg gccatgctta tctccatgcg gtagggtgcc gcacggttgc
2221 ggcaccatgc gcaatcagct gcaacttttc ggcagcgcga caacaattat gcgttgcgta
2281 aaagtggcag tcaattacag attttcttta acctacgcaa tgagctattg cggggggtgc
2341 cgcaatgagc tgttgcgtac cccccttttt taagttgttg atttttaagt ctttcgcatt
2401 tcgccctata tctagttctt tggtgcccaa agaagggcac ccctgcgggg ttcccccacg
2461 ccttcggcgc ggctccccct ccggcaaaaa gtggcccctc cggggcttgt tgatcgactg
2521 cgcggccttc ggccttgccc aaggtggcgc tgcccccttg gaacccccgc actcgccgcc
2581 gtgaggctcg gggggcaggc gggcgggctt cgccttcgac tgcccccact cgcataggct
2641 tgggtcgttc caggcgcgtc aaggccaagc cgctgcgcgg tcgctgcgcg agccttgacc
2701 cgccttccac ttggtgtcca accggcaagc gaagcgcgca ggccgcaggc cggaggcttt
2761 tccccagaga aaattaaaaa aattgatggg gcaaggccgc aggccgcgca gttggagccg
2821 gtgggtatgt ggtcgaaggc tgggtagccg gtgggcaatc cctgtggtca agctcgtggg
2881 caggcgcagc ctgtccatca gcttgtccag cagggttgtc cacgggccga gcgaagcgag
2941 ccagccggtg gccgctcgcg gccatcgtcc acatatccac gggctggcaa gggagcgcag
3001 cgaccgcgca gggcgaagcc cggagagcaa gcccgtaggg cgccgcagcc gccgtaggcg
3061 gtcacgactt tgcgaagcaa agtctagtga gtatactcaa gcattgagtg gcccgccgga
3121 ggcaccgcct tgcgctgccc ccgtcgagcc ggttggacac caaaagggag gggcaggcat
3181 ggcggcatac gcgatcatgc gatgcaagaa gctggcgaaa atgggcaacg tggcggccag
3241 tctcaagcac gcctaccgcg agcgcgagac gcccaacgct gacgccagca ggacgccaga
3301 gaacgagcac tgggcggcca gcagcaccga tgaagcgatg ggccgactgc gcgagttgct
3361 gccagagaag cggcgcaagg acgctgtgtt ggcggtcgag tacgtcatga cggccagccc
3421 ggaatggtgg aagtcggcca gccaagaaca gcaggcggcg ttcttcgaga aggcgcacaa
3481 gtggctggcg gacaagtacg gggcggatcg catcgtgacg gccagcatcc accgtgacga
3541 aaccagcccg cacatgaccg cgttcgtggt gccgctgacg caggacggca ggctgtcggc
3601 caaggagttc atcggcaaca aagcgcagat gacccgcgac cagaccacgt ttgcggccgc
3661 tgtggccgat ctagggctgc aacggggcat cgagggcagc aaggcacgtc acacgcgcat
3721 tcaggcgttc tacgaggccc tggagcggcc accagtgggc cacgtcacca tcagcccgca
3781 agcggtcgag ccacgcgcct atgcaccgca gggattggcc gaaaagctgg gaatctcaaa
3841 gcgcgttgag acgccggaag ccgtggccga ccggctgaca aaagcggttc ggcaggggta
3901 tgagcctgcc ctacaggccg ccgcaggagc gcgtgagatg cgcaagaagg ccgatcaagc
3961 ccaagagacg gcccgagacc ttcgggagcg cctgaagccc gttctggacg ccctggggcc
4021 gttgaatcgg gatatgcagg ccaaggccgc cgcgatcatc aaggccgtgg gcgaaaagct
4081 gctgacggaa cagcgggaag tccagcgcca gaaacaggcc cagcgccagc aggaacgcgg
4141 gcgcgcacat ttccccgaaa agtgccacct ggcggcgttg tgacaattta ccgaacaact
4201 ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta cttgggtcga
4261 tagctttatg cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct
4321 agggtcccca attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg
4381 gatcaattcc cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga
4441 tccttggagc ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg
4501 acccgcagtt gcaaaccctc actgatccgc cgagatgcgc cgcgtgcggc tgctggagat
4561 ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa
4621 gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc
4681 attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg
4741 tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa
4801 atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat
4861 ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg
4921 ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc
4981 gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc
5041 tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc
5101 aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc
5161 tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag
5221 acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg
5281 ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag
5341 cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag
5401 gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa
5461 gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag
5521 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg
5581 atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa
5641 gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat
5701 agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg
5761 gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca
5821 gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt
5881 gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctc gcgcagatca
5941 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
6001 tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
6061 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
6121 aagcgtgcat aataagccct acacaaattg ggagatatat catgaaaggc tggctttttc
6181 ttgttatcgc aatagttggc gaagtaatcg caacatccgc attaaaatct agcgagggct
6241 ttactaagct gatccggtgg atgacctttt gaatgacctt taatagatta tattactaat
6301 taattgggga ccctagaggt cccctttttt attttaaaaa ttttttcaca aaacggttta
6361 caagcataaa gctcaaggag atgg
//
