| 质粒名称: | pNK9 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31044 |
| 存储实验室: | Adam Bogdanove, Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pTC14 |
| 载体抗性: | 四环素 |
| 空载体大小: | 2989 bp |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | repeat NK9 |
| 物种来源: | Xanthamonas oryzae |
| 插入大小: | 118 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XbaI（未销毁） |
| 3' 克隆位点: | XhoI（未销毁） |
| 5' 测序引物 | pTC14_F2 (caagcctgattgggagaaaa) |
|
文献引用方法 |
|
| 材料和方法部分: | pNK9 was a gift from Adam Bogdanove & Daniel Voytas (Addgene plasmid # 31044 ; http://n2t.net/addgene:31044 ; RRID:Addgene_31044) |
| 参考文献部分: | Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pNK9 3113 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pNK9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3113)
AUTHORS Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller
JA, Somia NV, Bogdanove AJ, Voytas DF
TITLE Efficient design and assembly of custom TALEN and other TAL
effector-based constructs for DNA targeting.
JOURNAL Nucleic Acids Res. 2011 Apr 14. ():.
PUBMED 21493687
REFERENCE 2 (bases 1 to 3113)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3113
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 196..224
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS 272..1462
/codon_start=1
/gene="tet"
/product="tetracycline efflux protein"
/label=TcR
/note="confers resistance to tetracycline"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
primer_bind complement(624..643)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(1636..1655)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(1637..1655)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1660..1676)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1660..1676)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
rep_origin 1962..2550
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2451..2470
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
terminator 2880..2907
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 2999..3085
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
ORIGIN
1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca
241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg
601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa
781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg
841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga
1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
1741 tgcctcctct agaggtctcc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga
1801 ccatggcctg accccggacc aagtggtggc tatcgccagc aacaagggcg gcaagcaagc
1861 gctcggagac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
1921 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
1981 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
2041 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
2101 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
2161 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
2221 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
2281 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
2341 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
2401 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
2461 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
2521 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
2581 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
2641 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
2701 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
2761 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
2821 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
2881 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
2941 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
3001 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
3061 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gtt
//
