| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.83 ; pGL4.83[hRlucP/Puro] |
| 质粒类型: | 信号通路报告载体；哺乳动物载体；海肾荧光素酶报告载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 4815 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | hRlucP |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素(Puromycin) |
| 克隆菌株: | TOP10等常规菌株 |
| 宿主细胞（系）: | HEK293等 |
| 备注: | pGL4.83[hRlucP/Puro]载体是信号通路报告载体；无启动子；含海肾荧光素酶报告基因hRlucP。 |
| 产品目录号: | E7511 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pGL4.83[hRlucP/Puro]载体pGL4.83质粒基本信息
pGL4.83[hRlucP/Puro]质粒图谱pGL4.83载体图谱和pGL4.83[hRlucP/Puro]载体序列pGL4.83质粒序列多克隆位点信息
pGL4.83[hRlucP/Puro]质粒pGL4.83载体简介
The pGL4.83[hRlucP/Puro] Vector encodes the luciferase reporter gene hRlucP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for puromycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. It has also been engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.83[hRlucP/Puro] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice. The hRlucP reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by hRlucP responds more quickly and with a greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.
pGL4.83[hRlucP/Puro]质粒序列pGL4.83载体序列
LOCUS DQ188847 4815 bp DNA circular SYN 16-NOV-2005
DEFINITION Synthetic Renilla luciferase reporter vector pGL4.83[hRlucP/Puro],
complete sequence.
ACCESSION DQ188847
VERSION DQ188847.1 GI:76364318
KEYWORDS .
SOURCE Renilla luciferase reporter vector pGL4.83[hRlucP/Puro]
ORGANISM Renilla luciferase reporter vector pGL4.83[hRlucP/Puro]
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4815)
AUTHORS Stecha,P., Paguio,A. and Chauvin,F.
TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
Corporation
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4815)
AUTHORS Stecha,P., Paguio,A. and Chauvin,F.
TITLE Direct Submission
JOURNAL Submitted (31-AUG-2005) Scientific Communications, Promega
Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES Location/Qualifiers
source 1..4815
/organism="Renilla luciferase reporter vector
pGL4.83[hRlucP/Puro]"
/mol_type="other DNA"
/db_xref="taxon:346094"
misc_feature 1..70
/note="multiple cloning region"
CDS 100..1158
/note="synthetic Renilla luciferase; contains hPEST
destabilization sequence"
/codon_start=1
/product="hRlucP"
/protein_id="ABA41683.1"
/db_xref="GI:76364319"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSHGFPPEVEEQAAGTLPMSCAQ
ESGMDRHPAACASARINV"
regulatory 1198..1419
/regulatory_class="polyA_signal_sequence"
/note="SV40 late poly(A) signal"
regulatory 1467..1885
/regulatory_class="promoter"
/note="SV40 early enhancer/promoter"
CDS 1910..2509
/note="puromycin resistance"
/codon_start=1
/product="puromycin-N-acetyltransferase"
/protein_id="ABA41684.1"
/db_xref="GI:76364320"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
regulatory 2534..2582
/regulatory_class="polyA_signal_sequence"
/note="synthetic poly(A) signal"
primer_bind 2649..2668
/note="Reporter Vector primer 4 (RVprimer4) binding
region"
rep_origin 2906
/note="ColEI-derived plasmid replication origin"
gene complement(3697..4557)
/gene="bla"
CDS complement(3697..4557)
/gene="bla"
/note="ampicillin resistance"
/codon_start=1
/product="beta-lactamase"
/protein_id="ABA41685.1"
/db_xref="GI:76364321"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3697..4557
/regulatory_class="polyA_signal_sequence"
/note="synthetic poly(A) signal/transcriptional pause
site"
primer_bind 4764..4783
/note="Reporter Vector primer 3 (RVprimer3) binding
region"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
1021 aagaacgagc agaattctca cggcttccct cccgaggtgg aggagcaggc cgccggcacc
1081 ctgcccatga gctgcgccca ggagagcggc atggatagac accctgctgc ttgcgccagc
1141 gccaggatca acgtctaagg ccgcgactct agagtcgggg cggccggccg cttcgagcag
1201 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
1261 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
1321 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
1381 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtttg
1441 cgtattgggc gctcttccgc tgatctgcgc agcaccatgg cctgaaataa cctctgaaag
1501 aggaacttgg ttagctacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
1561 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
1621 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
1681 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
1741 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
1801 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
1861 aggcctaggc ttttgcaaaa agctcgattc ttctgacact agcgccacca tgaccgagta
1921 caagcctacc gtgcgcctgg ccactcgcga tgatgtgccc cgcgccgtcc gcactctggc
1981 cgccgctttc gccgactacc ccgctacccg gcacaccgtg gaccccgacc ggcacatcga
2041 gcgtgtgaca gagttgcagg agctgttcct gacccgcgtc gggctggaca tcggcaaggt
2101 gtgggtagcc gacgacggcg cggccgtggc cgtgtggact acccccgaga gcgttgaggc
2161 cggcgccgtg ttcgccgaga tcggcccccg aatggccgag ctgagcggca gccgcctggc
2221 cgcccagcag caaatggagg gcctgcttgc cccccatcgt cccaaggagc ctgcctggtt
2281 tctggccact gtaggagtga gccccgacca ccagggcaag ggcttgggca gcgccgtcgt
2341 gttgcccggc gtagaggccg ccgaacgcgc cggtgtgccc gcctttctcg aaacaagcgc
2401 accaagaaac cttccattct acgagcgcct gggcttcacc gtgaccgccg atgtcgaggt
2461 gcccgaggga cctaggacct ggtgtatgac acgaaaacct ggcgcctaat gatctagaac
2521 cggtcatggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
2581 tgttcgaact agatgctgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
2641 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
2701 actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct
2761 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
2821 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
2881 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
2941 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
3001 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
3061 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
3121 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
3181 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
3241 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
3301 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
3361 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
3421 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
3481 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
3541 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
3601 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
3661 ctgacagcgg ccgcaaatgc taaaccactg cagtggttac cagtgcttga tcagtgaggc
3721 accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta
3781 gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga tgccgcgaga
3841 gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga gggccgagcg
3901 aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct gtcgtgatgc
3961 tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg ctactggcat
4021 cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc agcggtcaag
4081 ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag ggcctccgat
4141 cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa
4201 ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt actcaaccaa
4261 gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt ctatacggga
4321 caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc gttcttcggg
4381 gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc ccactcttgc
4441 acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg caaaaacagg
4501 caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga tgctcatact
4561 cgtccttttt caatattatt gaagcattta tcagggttac tagtacgtct ctcaaggata
4621 agtaagtaat attaaggtac gggaggtatt ggacaggccg caataaaata tctttatttt
4681 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
4741 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
4801 ccagaacatt tctct
//
