| 质粒名称: | pAc-Neo-OVA |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 22533 |
| 存储实验室: | Michael Bevan |
|
载体骨架基本信息 |
|
| 载体名称: | pHBetaAPr-1-neo |
| 载体出品公司: | Kedes Lab |
| 空载体大小: | 10200 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | OVA |
| 物种来源: | G. gallus (chicken) |
| 插入大小: | 1700 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | HindIII(未破坏) |
| 3’克隆位点: | BamHI(未破坏) |
| 5’测序引物 | -- |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pAc-Neo-OVA was a gift from Michael Bevan (Addgene plasmid # 22533 ; http://n2t.net/addgene:22533 ; RRID:Addgene_22533) |
| 参考文献部分: | Introduction of soluble protein into the class I pathway of antigen processing and presentation. Moore MW, Carbone FR, Bevan MJ. Cell. 1988 Sep 9. 54(6):777-85. 10.1016/S0092-8674(88)91043-4 PubMed 3261634 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAc-Neo-OVA 12268 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAc-Neo-OVA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12268)
AUTHORS Moore MW, Carbone FR, Bevan MJ
TITLE Introduction of soluble protein into the class I pathway of antigen
processing and presentation.
JOURNAL Cell. 1988 Sep 9. 54(6):777-85.
PUBMED 3261634
REFERENCE 2 (bases 1 to 12268)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12268
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 4384..4440
/label=MCS
/note="pUC18/19 multiple cloning site"
CDS 4466..5626
/codon_start=1
/product="chicken ovalbumin"
/label=ovalbumin
/translation="MGSIGAASMEFCFDVFKELKVHHANENIFYCPIAIMSALAMVYLG
AKDSTRTQINKVVRFDKLPGFGDSIEAQCGTSVNVHSSLRDILNQITKPNDVYSFSLAS
RLYAEERYPILPEYLQCVKELYRGGLEPINFQTAADQARELINSWVESQTNGIIRNVLQ
PSSVDSQTAMVLVNAIVFKGLWEKAFKDEDTQAMPFRVTEQESKPVQMMYQIGLFRVAS
MASEKMKILELPFASGTMSMLVLLPDEVSGLEQLESIINFEKLTEWTSSNVMEERKIKV
YLPRMKMEEKYNLTSVLMAMGITDVFSSSANLSGISSAESLKISQAVHAAHAEINEAGR
EVVGSAEAGVDAASVSEEFRADHPFLFCIKHIATNAVLFFGRCVSP"
polyA_signal 6323..6457
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6600..6622)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6833..6850)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7004..7592)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7084..7103)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7763..8623)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8386..8405
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8624..8728)
/gene="bla"
/label=AmpR promoter
primer_bind 8796..8814
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(8834..8854)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8839..9168
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9019..9154
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9081..9100
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 9529..10323
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(9583..9602)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 10193..10212
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
intron 10743..10808
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 10938..10958
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 11383..11517
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 aattccgact ttcaaacagc ctttggactc aggccttggg gacattccag gggacccttt
61 accagggagg ggcagtgtga ggcaggaggc agggctgccg cctcagggac cctgagccta
121 gagtccttcc caccgcattt ggggacttga gcacttcacc agtcctgact cagtttccta
181 gtctgtgaat gggggtgtgt ctcctggaat gttgcccccc ctcattcatt cattctcctc
241 tcactgagca cctaccgtgt tcagggtccc tgtcctccta gagctaactc aagtcttgga
301 gggaagctct gtctcagttc cttcctctac aaaatggagc ttgaggacgg gcacggtggc
361 tcatgcctgt aatcccagga ctttgggagg ctgaggtggg aggatcgctt gagccccccg
421 gagctggaga ccagcctggg caacatggtg agaagccata cctctacaga taatttaaaa
481 attagccagg cttggaagtg tgtgcctata gtcccagcta ctcgtgaagg ctttgagccc
541 aggaggtcga ggctgcagtg agctgtgatc gcactccagc ctgggcatca cagcaagacc
601 ttgtatcaaa aatagtaata ataataaaaa ggaggttgga ttccctcctg gcaggatagg
661 gagggcgctg cagtgcccag ggcaaggtgg ctgggtggtt gttttgcggg agggccaagg
721 agtggtccct gggtctgcgc tgtaagagtt ggttgcctgg aggccttgca gggtgggggt
781 gtcaccaaag caaaggcttg gaggggaaaa aacaaaagtc ccagccaggc aggtggagtc
841 cccttggctg agttcaaccg agggttctcc gggggctgcg tgcgtgcccc agtgacagct
901 ccgaaagctc ccttacaggg caaagttccc aagcacagaa gagaacctgt tcacttctcc
961 cctgctcggc ccgccccctg gccaggcacc tctacttcct cttttcctgc tccgctgctt
1021 gctttctctc ttcagctcct ccctgcccct caccccaggc tgctcggcca cctccaacct
1081 gccacctgag gacacccagg cagtcactca ttcaacagcg aggagccctg gggtgggtgt
1141 agtgggaagg agtgggggtg acggagaccc tgggagggct cgcagcctgg tggctgaggc
1201 ccagttctaa atgccagctg caagccttgg tctgaggtag ggaggaaggc gtggctgcag
1261 aggctaaaac gcttccccaa agaggggctt tctgggatgg gacttgaagg gtgcatagga
1321 gagcactagg aagtggccgc tgcagacaga gggaaccaca agccaggagg acaggccagg
1381 aatgctgcag cccggggcgg ggtggggctg gagctcctgt ctcttggcca gctgaatgga
1441 ggcccagcgg caacacaggt cctgcctggg gatcaggtct gctctgcacc ccaccttgct
1501 gcctggagcc gcccacctga caacctctca tccctgctct gtagatccgg tcccatcccc
1561 actgcccacc ccaccccccc agcactccac ccagttcaac gttccacgaa cccccagaac
1621 cagccctcat caacaggcag caagaagggc cccccgccca tcgccccaca acgccagccg
1681 ggtgaactgt agcgttggca ggtcctgagg cagctggcaa gacgcctgca gctgaaagat
1741 acaaggccag ggacaggaca gtcccatccc caggaggcag ggagtataca ggctggggaa
1801 gtttgccctt gcgtggggtg gtgatggagg aggctcagca agtcttctgg actgtgaacc
1861 tgtgtctgcc actgtgtgct gggtggtggt catctttccc accaggctgt ggcctctgca
1921 accttcaagg gaggagcagg tcccattggc tgagcacagc cttgtaccgt gaactggaac
1981 aagcagcctc cttcctggcc acaggttcca tgtccttata tggactcatc tttgcctatt
2041 gcgacacaca ctcaatgaac acctactacg cgctgcaaag agccccgcag gcctgaggtg
2101 cccccacctc accactcttc ctatttttgt gtaaaaatcc agcttcttgt caccacctcc
2161 aaggaggggg aggaggagga aggcaggttc ctctaggctg agccgaatgc ccctctgtgg
2221 tcccacgcca ctgatcgctg catgcccacc acctgggtac acacagtctg tgattcccgg
2281 agcagaacgg accctgccca cccggtcttg tgtgctactc agtggacaga cccaaggcaa
2341 gaaagggtga caaggacagg gtcttcccag gctggctttg agttcctagc accgccccgc
2401 ccccaatcct ctgtggcaca tggagtcttg gtccccagag tcccccagcg gcctccagat
2461 ggtctgggag ggcagttcag ctgtggctgc gcatagcaga catacaacgg acggtgggcc
2521 cagacccagg ctgtgtagac ccagcccccc cgccccgcag tgcctaggtc acccactaac
2581 gccccaggcc tggtcttggc tgggcgtgac tgttaccctc aaaagcaggc agctccaggg
2641 taaaaggtgc cctgccctgt agagcccacc ttccttccca gggctgcggc tgggtaggtt
2701 tgtagccttc atcacgggcc acctccagcc actggaccgc tggcccctgc cctgtcctgg
2761 ggagtgtggt cctgcgactt ctaagtggcc gcaagccacc tgactccccc aacaccacac
2821 tctacctctc aagcccaggt ctctccctag tgacccaccc agcacattta gctagctgag
2881 ccccacagcc agaggtcctc aggccctgct ttcagggcag ttgctctgaa gtcggcaagg
2941 gggagtgact gcctggccac tccatgccct ccaagagctc cttctgcagg agcgtacaga
3001 acccagggcc ctggcacccg tgcagaccct ggcccacccc acctgggcgc tcagtgccca
3061 agagatgtcc acacctagga tgtcccgcgg tgggtggggg gcccgagaga cgggcaggcc
3121 gggggcaggc ctggccatgc ggggccgaac cgggcactgc ccagcgtggg gcgcgggggc
3181 cacggcgcgc gcccccagcc cccgggccca gcaccccaag gcggccaacg ccaaaactct
3241 ccctcctcct cttcctcaat ctcgctctcg ctcttttttt ttttcgcaaa aggaggggag
3301 agggggtaaa aaaatgctgc actgtgcggc gaagccggtg agtgagcggc gcggggccaa
3361 tcagcgtgcg ccgttccgaa agttgccttt tatggctcga gcggccgcgg cggcgcccta
3421 taaaacccag cggcgcgacg cgccaccacc gccgagaccg cgtccgcccc gcgagcacag
3481 agcctcgcct ttgccgatcc gccgcccgtc cacacccgcc gccaggtaag cccggccagc
3541 cgaccggggc atgcggccgc ggccccttcg cccgtgcaga gccgccgtct gggccgcagc
3601 ggggggcgca tgggggggga accggaccgc cgtggggggc gcgggagaag cccctgggcc
3661 tccggagatg ggggacaccc cacgccagtt cggaggcgcg aggccgcgct cgggaggcgc
3721 gctccggggg tgccgctctc ggggcggggg caaccggcgg ggtctttgtc tgagccgggc
3781 tcttgccaat ggggatcgca gggtgggcgc ggcgtagccc ccgccaggcc cggtgggggc
3841 tggggcgcca ttgcgcgtgc gcgctggtcc tttgggcgct aactgcgtgc gcgctgggaa
3901 ttggcgctaa ttgcgcgtgc gcgctgggac tcaaggcgct aattgcgcgt gcgttctggg
3961 gcccggggtg ccgcggcctg ggctggggcg aaggcgggct cggccggaag gggtggggtc
4021 gccgcggctc ccgggcgctt gcgcgcactt cctgcccgag ccgctggccg cccgagggtg
4081 tggccgctgc gtgcgcgcgc gccgacccgg cgctgtttga accgggcgga ggcggggctg
4141 gcgcccggtt gggagggggt tggggcctgg cttcctgccg cgcgccgcgg ggacgcctcc
4201 gaccagtgtt tgccttttat ggtaataacg cggccggccc ggcttccttt gtccccaatc
4261 tgggcgcgcg ccggcgcccc ctggcggcct aaggactcgg cgcgccggaa gtggccaggg
4321 cgggggcgac ttcggctcac agcgcgcccg gctattctcg caggatcagt cgacctgcag
4381 cccaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc
4441 ctcgaaagac aactcagagt tcaccatggg ctccatcggt gcagcaagca tggaattttg
4501 ttttgatgta ttcaaggagc tcaaagtcca ccatgccaat gagaacatct tctactgccc
4561 cattgccatc atgtcagctc tagccatggt atacctgggt gcaaaagaca gcaccaggac
4621 acaaataaat aaggttgttc gctttgataa acttccagga ttcggagaca gtattgaagc
4681 tcagtgtggc acatctgtaa acgttcactc ttcacttaga gacatcctca accaaatcac
4741 caaaccaaat gatgtttatt cgttcagcct tgccagtaga ctttatgctg aagagagata
4801 cccaatcctg ccagaatact tgcagtgtgt gaaggaactg tatagaggag gcttggaacc
4861 tatcaacttt caaacagctg cagatcaagc cagagagctc atcaattcct gggtagaaag
4921 tcagacaaat ggaattatca gaaatgtcct tcagccaagc tccgtggatt ctcaaactgc
4981 aatggttctg gttaatgcca ttgtcttcaa aggactgtgg gagaaagcat ttaaggatga
5041 agacacacaa gcaatgcctt tcagagtgac tgagcaagaa agcaaacctg tgcagatgat
5101 gtaccagatt ggtttattta gagtggcatc aatggcttct gagaaaatga agatcctgga
5161 gcttccattt gccagtggga caatgagcat gttggtgctg ttgcctgatg aagtctcagg
5221 ccttgagcag cttgagagta taatcaactt tgaaaaactg actgaatgga ccagttctaa
5281 tgttatggaa gagaggaaga tcaaagtgta cttacctcgc atgaagatgg aggaaaaata
5341 caacctcaca tctgtcttaa tggctatggg cattactgac gtgtttagct cttcagccaa
5401 tctgtctggc atctcctcag cagagagcct gaagatatct caagctgtcc atgcagcaca
5461 tgcagaaatc aatgaagcag gcagagaggt ggtagggtca gcagaggctg gagtggatgc
5521 tgcaagcgtc tctgaagaat ttagggctga ccatccattc ctcttctgta tcaagcacat
5581 cgcaaccaac gccgttctct tctttggcag atgtgtttcc ccttaaaaag aagaaagctg
5641 aaaaactctg tcccttccaa caagacccag agcactgtag tatcaggggt aaaatgaaaa
5701 gtatgttatc tgctgcatcc agacttcata aaagctggag cttaatctag aaaaaaaatc
5761 agaaagaaat tacactgtga gaacaggtgc aattcacttt tcctttacac agagtaatac
5821 tggtaactca tggatgaagg cttaagggaa tgaaattgga ctcacagtac tgagtcatca
5881 cactgaaaaa tgcaacctga tacatcagca gaaggtttat gggggaaaaa tgcagccttc
5941 caattaagcc agatatctgt atgaccaagc tgctccagaa ttagtcactc aaaatctctc
6001 agattaaatt atcaactgtc accaaccatt cctatgctga caaggcaatt gcttgttctc
6061 tgtgttcctg atactacaag gctcttcctg acttcctaaa gatgcattat aaaaatctta
6121 taattcacat ttctccctaa actttgactc aatcatggta tgttggcaaa tatggtatat
6181 tactattcaa attgttttcc ttgtacccat atgtaatggg tcttgtgaat gtgctctttt
6241 gttcctttaa tcataataaa aacatgttta agcaaaaaaa aaaaaaaaaa aaaaaaaaag
6301 gaattcgccc atgggcgccc cagatccaga catgataaga tacattgatg agtttggaca
6361 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
6421 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
6481 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
6541 atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
6601 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
6661 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
6721 cagtcacgta gcgggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
6781 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
6841 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
6901 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
6961 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
7021 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
7081 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
7141 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
7201 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
7261 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
7321 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
7381 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
7441 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
7501 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
7561 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
7621 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
7681 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
7741 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
7801 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
7861 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
7921 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
7981 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
8041 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
8101 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
8161 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
8221 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
8281 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
8341 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
8401 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
8461 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
8521 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
8581 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
8641 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
8701 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
8761 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
8821 ttatcatcgc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
8881 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
8941 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
9001 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
9061 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
9121 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctagcttca
9181 cgctgccgca agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc
9241 agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg
9301 gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta
9361 gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt
9421 aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg
9481 cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
9541 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
9601 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
9661 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
9721 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
9781 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
9841 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
9901 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
9961 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
10021 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
10081 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
10141 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
10201 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
10261 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
10321 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
10381 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
10441 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccgggc
10501 tcgatcccct cgcgagttgg ttcagctgct gcctgaggct ggacgacctc gcggagttct
10561 accggcagtg caaatccgtc ggcatccagg aaaccagcag cggctatccg cgcatccatg
10621 cccccgaact gcaggagtgg ggaggcacga tggccgcttt ggtccggatc tttgtgaagg
10681 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
10741 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
10801 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
10861 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
10921 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
10981 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
11041 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
11101 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
11161 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
11221 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
11281 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
11341 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
11401 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
11461 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcgat
11521 ccccaggaag ctcctctgtg tcctcataaa ccctaacctc ctctacttga gaggacattc
11581 caatcatagg ctgcccatcc accctctgtg tcctcctgtt aattaggtca cttaacaaaa
11641 aggaaattgg gtaggggttt ttcacagacc gctttctaag ggtaatttta aaatatctgg
11701 gaagtccctt ccactgctgt gttccagaag tgttggtaaa cagcccacaa atgtcaacag
11761 cagaaacata caagctgtca gctttgcaca agggcccaac accctgctca tcaagaagca
11821 ctgtggttgc tgtgttagta atgtgcaaaa caggaggcac attttcccca cctgtgtagg
11881 ttccaaaata tctagtgttt tcatttttac ttggatcagg aacccagcac tccactggat
11941 aagcattatc cttatccaaa acagccttgt ggtcagtgtt catctgctga ctgtcaactg
12001 tagcattttt tggggttaca gtttgagcag gatatttggt cctgtagttt gctaacacac
12061 cctgcagctc caaaggttcc ccaccaacag caaaaaaatg aaaatttgac ccttgaatgg
12121 gttttccagc accattttca tgagtttttt gtgtccctga atgcaagttt aacatagcag
12181 ttaccccaat aacctcagtt ttaacagtaa cagcttccca catcaaaata tttccacagg
12241 ttaagtcctc atttaaatta ggcaaagg
//
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