pCEP4-YFP

LOCUS       pCEP4-YFP               10902 bp DNA     circular SYN 12-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10902)
  AUTHORS   Biofeng
  TITLE     Direct Submission
  JOURNAL   Exported Jul 12, 2023 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      626..635
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             632..1351
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    1388..1522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2245..4034
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4336..6261)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     promoter        6782..6886
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6887..7747
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7918..8506
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        8937..9082
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9125..10162
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     polyA_signal    10204..10252
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gtaccgccac catggtgagc aagggcgagg agctgttcac
      661 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      721 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      781 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca
      841 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      901 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      961 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1021 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1081 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     1141 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1201 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
     1261 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1321 cactctcggc atggacgagc tgtacaagta aaagcttgct agcggccgct cgaggccggc
     1381 aaggccggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     1441 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     1501 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     1561 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     1621 ttatgatccg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     1681 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     1741 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga ggtcgactct agaggatcga
     1801 tgccccgccc cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca
     1861 gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt
     1921 gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat
     1981 ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt
     2041 ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc
     2101 tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat
     2161 cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt
     2221 tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata
     2281 ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg
     2341 aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca
     2401 cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg
     2461 gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc
     2521 attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga
     2581 ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca
     2641 gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg caataaatac
     2701 tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca
     2761 agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc
     2821 tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga
     2881 accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg
     2941 actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc
     3001 cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca
     3061 gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga
     3121 ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc
     3181 cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct
     3241 ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata
     3301 ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca
     3361 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     3421 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     3481 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     3541 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata
     3601 gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata
     3661 tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     3721 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     3781 tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata
     3841 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta
     3901 tccgggtagc atatgctatc ctcatgcata tacagtcagc atatgatacc cagtagtaga
     3961 gtgggagtgc tatcctttgc atatgccgcc acctcccaag ggggcgtgaa ttttcgctgc
     4021 ttgtcctttt cctgctggtt gctcccattc ttaggtgaat ttaaggaggc caggctaaag
     4081 ccgtcgcatg tctgattgct caccaggtaa atgtcgctaa tgttttccaa cgcgagaagg
     4141 tgttgagcgc ggagctgagt gacgtgacaa catgggtatg cccaattgcc ccatgttggg
     4201 aggacgaaaa tggtgacaag acagatggcc agaaatacac caacagcacg catgatgtct
     4261 actggggatt tattctttag tgcgggggaa tacacggctt ttaatacgat tgagggcgtc
     4321 tcctaacaag ttacatcact cctgcccttc ctcaccctca tctccatcac ctccttcatc
     4381 tccgtcatct ccgtcatcac cctccgcggc agccccttcc accataggtg gaaaccaggg
     4441 aggcaaatct actccatcgt caaagctgca cacagtcacc ctgatattgc aggtaggagc
     4501 gggctttgtc ataacaaggt ccttaatcgc atccttcaaa acctcagcaa atatatgagt
     4561 ttgtaaaaag accatgaaat aacagacaat ggactccctt agcgggccag gttgtgggcc
     4621 gggtccaggg gccattccaa aggggagacg actcaatggt gtaagacgac attgtggaat
     4681 agcaagggca gttcctcgcc ttaggttgta aagggaggtc ttactacctc catatacgaa
     4741 cacaccggcg acccaagttc cttcgtcggt agtcctttct acgtgactcc tagccaggag
     4801 agctcttaaa ccttctgcaa tgttctcaaa tttcgggttg gaacctcctt gaccacgatg
     4861 ctttccaaac caccctcctt ttttgcgcct gcctccatca ccctgacccc ggggtccagt
     4921 gcttgggcct tctcctgggt catctgcggg gccctgctct atcgctcccg ggggcacgtc
     4981 aggctcacca tctgggccac cttcttggtg gtattcaaaa taatcggctt cccctacagg
     5041 gtggaaaaat ggccttctac ctggaggggg cctgcgcggt ggagacccgg atgatgatga
     5101 ctgactactg ggactcctgg gcctcttttc tccacgtcca cgacctctcc ccctggctct
     5161 ttcacgactt ccccccctgg ctctttcacg tcctctaccc cggcggcctc cactacctcc
     5221 tcgaccccgg cctccactac ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc
     5281 tccacctcct gctcctgccc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc
     5341 ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc
     5401 tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     5461 tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc
     5521 tgctcctgcc cctcctgctc ctgcccctcc tgctcctgcc cctcctgctc ctgcccctcc
     5581 tgcccctcct gcccctcctc ctgctcctgc ccctcctgct cctgcccctc ctgcccctcc
     5641 tgcccctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc
     5701 tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc
     5761 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc
     5821 ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
     5881 tcctgctcct gcccctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     5941 tcctcctgct cctgcccctc ctgctcctgc ccctcccgct cctgctcctg ctcctgttcc
     6001 accgtgggtc cctttgcagc caatgcaact tggacgtttt tggggtctcc ggacaccatc
     6061 tctatgtctt ggccctgatc ctgagccgcc cggggctcct ggtcttccgc ctcctcgtcc
     6121 tcgtcctctt ccccgtcctc gtccatggtt atcaccccct cttctttgag gtccactgcc
     6181 gccggagcct tctggtccag atgtgtctcc cttctctcct aggccatttc caggtcctgt
     6241 acctggcccc tcgtcagaca tgattcacac taaaagagat caatagacat ctttattaga
     6301 cgacgctcag tgaatacagg gagtgcagac tcctgccccc tccaacagcc cccccaccct
     6361 catccccttc atggtcgctg tcagacagat ccaggtctga aaattcccca tcctccgaac
     6421 catcctcgtc ctcatcacca attactcgca gcccggaaaa ctcccgctga acatcctcaa
     6481 gatttgcgtc ctgagcctca agccaggcct caaattcctc gtcccccttt ttgctggacg
     6541 gtagggatgg ggattctcgg gacccctcct cttcctcttc aaggtcacca gacagagatg
     6601 ctactggggc aacggaagaa aagctgggtg cggcctgtga ggatcagctt atcgatgata
     6661 agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     6721 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     6781 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6841 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6901 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6961 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7021 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7081 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     7141 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7201 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7261 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7321 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7381 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc
     7441 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7501 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7561 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7621 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7681 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7741 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7801 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7861 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7921 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7981 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8041 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     8101 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8161 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8221 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8281 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8341 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8401 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8461 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8521 ggccttttta cggttcctgg ccttttgctg gccttgaagc tgtccctgat ggtcgtcatc
     8581 tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag
     8641 aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag
     8701 cgcgtcggcc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg
     8761 ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt
     8821 cttggagtgg tgaatccgtt agcgaggtgc cgccctgctt catccccgtg gcccgttgct
     8881 cgcgtttgct ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt tctatgatga
     8941 cacaaacccc gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc
     9001 ggcgcggtcc gaggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc
     9061 gaccctgcag cgacccgctt aacagcgtca acagcgtgcc gcagatcccg gggggcaatg
     9121 agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     9181 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     9241 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     9301 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     9361 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     9421 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     9481 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     9541 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     9601 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     9661 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     9721 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     9781 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     9841 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     9901 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     9961 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
    10021 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
    10081 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccggatcg
    10141 ggagatgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
    10201 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc
    10261 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
    10321 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
    10381 ctcgcagcca acgtcggggc ggcaggccct gccatagcca ctggccccgt gggttaggga
    10441 cggggtcccc catggggaat ggtttatggt tcgtgggggt tattattttg ggcgttgcgt
    10501 ggggtcaggt ccacgactgg actgagcaga cagacccatg gtttttggat ggcctgggca
    10561 tggaccgcat gtactggcgc gacacgaaca ccgggcgtct gtggctgcca aacacccccg
    10621 acccccaaaa accaccgcgc ggatttctgg cgtgccaagc tagtcgacca attctcatgt
    10681 ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag gcgcagaact
    10741 ggtaggtatg gaagatctat acattgaatc aatattggca attagccata ttagtcattg
    10801 gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata
    10861 atatgtacat ttatattggc tcatgtccaa tatgaccgcc at
//