| 质粒名称: | 3XMEF2-luc |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 32967 |
| 存储实验室: | Ron Prywes |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3-promoter modified |
| 载体出品公司: | Promega |
| 空载体大小: | 5000 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | MEF2 binding sites |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | MEF2 |
| 标签/融合蛋白: | Luciferase（主干上的C端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | RVprimer3 |
| 3’测序引物 | Luc_N_Rev |
|
文献引用方法 |
|
| 材料和方法部分: | 3XMEF2-luc was a gift from Ron Prywes (Addgene plasmid # 32967 ; http://n2t.net/addgene:32967 ; RRID:Addgene_32967) |
买家导航
3XMEF2luc载体基本信息
3XMEF2luc载体图谱质粒图谱和多克隆位点信息
3XMEF2luc载体简介
3XMEF2luc载体序列
LOCUS 3XMEF2-luc 5943 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a
c-fos minimal promoter.
ACCESSION .
VERSION .
KEYWORDS 3XMEF2-luc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5943)
TITLE Prywes Lab Plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5943)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5943
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 152..171
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
polyA_signal 188..322
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
polyA_signal 346..480
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(905..925)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
intron 1055..1120
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS complement(1371..3023)
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/label=luciferase
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
primer_bind 2939..2957
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 3248..3382
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
polyA_signal 3476..3610
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3954..3971)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4125..4713)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4205..4224)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4884..5744)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5507..5526
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5745..5849)
/gene="bla"
/label=AmpR promoter
primer_bind 5917..5935
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
61 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
181 caccatagat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
241 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
301 tataagctgc aataaacaag ttccgggtac cgggcccccc ctcgagatcc agacatgata
361 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
421 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
481 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
541 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac
601 tatacatcaa atattcctta ttaacccctt tacaaattaa aaagctaaag gtacacaatt
661 tttgagcata gttattaata gcagacactc tatgcctgtg tggagtaaga aaaaacagta
721 tgttatgatt ataactgtta tgcctactta taaaggttac agaatatttt tccataattt
781 tcttgtatag cagtgcagct ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc
841 tattactaaa cacagcatga ctcaaaaaac ttagcaattc tgaaggaaag tccttggggt
901 cttctacctt tctcttcttt tttggaggag tagaatgttg agagtcagca gtagcctcat
961 catcactaga tggcatttct tctgagcaaa acaggttttc ctcattaaag gcattccacc
1021 actgctccca ttcatcagtt ccataggttg gaatctaaaa tacacaaaca attagaatca
1081 gtagtttaac acattataca cttaaaaatt ttatatttac cttagagctt taaatctctg
1141 taggtagttt gtccaattat gtcacaccac agaagtaagg ttccttcaca aagatcctct
1201 agagcggccg ccaccgcggt ggagctcgaa ttccttttta gttacctata cctgatttta
1261 ttattcgtta aaatagtaca gtgacccaca attacaaaat taccattcat caatttgcat
1321 ataatattac aatagctaag aatttcgtca tcgctgaata cagttacatt ttacaatttg
1381 gactttccgc ccttcttggc ctttatgagg atctctctga tttttcttgc gtcgagtttt
1441 ccggtaagac ctttcggtac ttcgtccaca aacacaactc ctccgcgcaa ctttttcgcg
1501 gttgttactt gactggcgac gtaatccacg atctcttttt ccgtcatcgt ctttccgtgc
1561 tccaaaacaa caacggcggc gggaagttca ccggcgtcat cgtcgggaag acctgccacg
1621 cccgcgtcga agatgttggg gtgttgtaac aatatcgatt ccaattcagc gggggccacc
1681 tgatatcctt tgtatttaat taaagacttc aagcggtcaa ctatgaagaa gtgttcgtct
1741 tcgtcccagt aagctatgtc tccagaatgt agccatccat ccttgtcaat caaggcgttg
1801 gtcgcttccg gattgtttac ataaccggac ataatcatag gtcctctgac acataattcg
1861 cctctctgat taacgcccag cgttttcccg gtatccagat ccacaacctt cgcttcaaaa
1921 aatggaacaa ctttaccgac cgcgcccggt ttatcatccc cctcgggtgt aatcagaata
1981 gctgatgtag tctcagtgag cccatatcct tgtcgtatcc ctggaagatg gaagcgtttt
2041 gcaaccgctt ccccgacttc tttcgaaaga ggtgcgcccc cagaagcaat ttcgtgtaaa
2101 ttagataaat cgtatttgtc aatcagagtg cttttggcga agaatgaaaa tagggttggt
2161 actagcaacg cactttgaat tttgtaatcc tgaagggatc gtaaaaacag ctcttcttca
2221 aatctataca ttaagacgac tcgaaatcca catatcaaat atccgagtgt agtaaacatt
2281 ccaaaaccgt gatggaatgg aacaacactt aaaatcgcag tatccggaat gatttgattg
2341 ccaaaaatag gatctctggc atgcgagaat ctgacgcagg cagttctatg cggaagggcc
2401 acacccttag gtaacccagt agatccagag gaattcatta tcagtgcaat tgttttgtca
2461 cgatcaaagg actctggtac aaaatcgtat tcattaaaac cgggaggtag atgagatgtg
2521 acgaacgtgt acatcgactg aaatccctgg taatccgttt tagaatccat gataataatt
2581 ttctggatta ttggtaattt tttttgcacg ttcaaaattt tttgcaaccc ctttttggaa
2641 acaaacacta cggtaggctg cgaaatgttc atactgttga gcaattcacg ttcattataa
2701 atgtcgttcg cgggcgcaac tgcaactccg ataaataacg cgcccaacac cggcataaag
2761 aattgaagag agttttcact gcatacgacg attctgtgat ttgtattcag cccatatcgt
2821 ttcatagctt ctgccaaccg aacggacatt tcgaagtatt ccgcgtacgt gatgttcacc
2881 tcgatatgtg catctgtaaa agcaattgtt ccaggaacca gggcgtatct cttcatagcc
2941 ttatgcagtt gctctccagc ggttccatcc tctagaggat agaatggcgc cgggcctttc
3001 tttatgtttt tggcgtcttc catggagatc ctctagagtc gactctagag gccgccggct
3061 cagtcttggc ttctcagttg ctcgctgcag atgcggttgg agtacgaggc gccgcagcca
3121 ctgcttttat aacaagcgtt ttatgaatga gtgcagatct cgagggctat ttttagggcc
3181 tcgagggcta tttttagggc ctcgagggct atttttagca agcttgggct gcaggtcgac
3241 tctagaggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
3301 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
3361 tataagctgc aataaacaag ttaacaacaa ttgcattcat tttatgtttc aggttcaggg
3421 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtgata tggctgatcc
3481 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
3541 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
3601 taaacaagtt aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
3661 ggttttttaa agcaagtaaa acctctacaa atgtgatatg gctgatcctg cctcgcgcgt
3721 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
3781 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
3841 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatct gcattaatga
3901 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
3961 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
4021 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
4081 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
4141 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
4201 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
4261 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
4321 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
4381 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
4441 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
4501 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
4561 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
4621 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
4681 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
4741 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
4801 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
4861 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
4921 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
4981 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
5041 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
5101 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
5161 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
5221 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
5281 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
5341 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
5401 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
5461 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
5521 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
5581 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
5641 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
5701 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
5761 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
5821 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
5881 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
5941 cgt
//
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