| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pDONR221-P5P4 |
| 质粒类型: | Mltisite-Gateway载体；DONR载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | 无 |
| 克隆方法: | Gateway；BP反应 |
| 载体大小: | 4726 bp |
| 5' 测序引物及序列: | M13/pUC fwd 5'CCCAGTCACGACGTTGTAAAACG 3' |
| 3' 测序引物及序列: | M13/pUC rev 5' AGCGGATAACAATTTCACACAGG 3' |
| 载体标签: | 无 |
| 载体抗性: | 卡那霉素，氯霉素（仅空载体） |
| 真核筛选标记: | 无 |
| 克隆菌株: | 阳性筛选用DB3.1 ，阴性筛选用OmniMAX2-T1 |
| 备注: |
pDONR221-P5P2载体是MultiSite Gateway载体， 目标载体如 pAd/PL-DEST、pcDNA6/V5-PL-DEST载体，是研究启动子的理想工具。 |
| 产品目录号: | 12537-102 |
| 病毒/非病毒: | 非病毒 |
买家导航
pDONR221-P5P4载体质粒基本信息
pDONR221-P5P4质粒图谱载体图谱和pDONR221-P5P4载体序列质粒序列多克隆位点信息
pDONR221-P5P4质粒载体简介
pDONR221-P5P4质粒序列载体序列
LOCUS Exported 4726 bp ds-DNA circular SYN 10-AUG-2016
DEFINITION synthetic circular DNA
KEYWORDS pDONR221-P5P4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4726)
AUTHORS Biofeng Lab.
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..4726
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 268..295
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 387..473
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 537..553
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 570..801
/gene="mutant version of attP"
/bound_moiety="BP Clonase(TM)"
/note="attP5"
/note="recombination site for the Gateway(R) BP reaction"
CDS complement(1149..1454)
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/note="ccdB"
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
CDS complement(1804..2457)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG"
promoter complement(2458..2560)
/note="cat promoter"
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
protein_bind complement(2705..2936)
/gene="mutant version of attP"
/bound_moiety="BP Clonase(TM)"
/note="attP4"
/note="recombination site for the Gateway(R) BP reaction"
promoter complement(2968..2986)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2991..3007)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 3120..3929
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 4076..4664
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaaa
661 agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
721 aaaacagact acataatact gtaaaacaca acatatccag tcactatgaa tcaactactt
781 agatggtatt agtgacctgt agtcgaccga cagccttcca aatgttcttc tcaaacggaa
841 tcgtcgtatc cagcctactc gctattgtcc tcaatgccgt attaaatcat aaaaagaaat
901 aagaaaaaga ggtgcgagcc tcttttttgt gtgacaaaat aaaaacatct acctattcat
961 atacgctagt gtcatagtcc tgaaaatcat ctgcatcaag aacaatttca caactcttat
1021 acttttctct tacaagtcgt tcggcttcat ctggattttc agcctctata cttactaaac
1081 gtgataaagt ttctgtaatt tctactgtat cgacctgcag actggctgtg tataagggag
1141 cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg
1201 tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg
1261 gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggagact
1321 ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca
1381 ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa
1441 accttaaact gcatttcacc agcccctgtt ctcgtcagca aaagagccgt tcatttcaat
1501 aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt cggcacgcag
1561 acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga catcatatat
1621 gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg cttcatagca
1681 tacctctttt tgacatactt cgggtataca tatcagtata tattcttata ccgcaaaaat
1741 cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gccggatcca cgcggcgttt
1801 acgccccccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
1861 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
1921 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
1981 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
2041 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
2101 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
2161 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
2221 gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa gaatgtgaat
2281 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
2341 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
2401 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
2461 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
2521 ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccaaaa
2581 gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt ctgcgaagtg
2641 atcttccgtc acaggtattt attcggcgca aagtgcgtcg ggtgatgctg ccaacttagt
2701 cgactacagg tcactaatac catctaagta gttgattcat agtgactgga tatgttgtgt
2761 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
2821 atcattttac gtttctcgtt caacttttct atacaaagtt ggcattataa gaaagcattg
2881 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
2941 tccagctgca gggcggccgc gatatcccct atagtgagtc gtattacatg gtcatagctg
3001 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
3061 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
3121 tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt
3181 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct
3241 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
3301 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
3361 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
3421 gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
3481 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
3541 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
3601 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
3661 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
3721 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
3781 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
3841 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
3901 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacactggc
3961 agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga
4021 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
4081 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
4141 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
4201 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
4261 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
4321 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
4381 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
4441 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
4501 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
4561 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
4621 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
4681 cctttttacg gttcctggcc ttttgctggc cttttgctca catgtt
//
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