| 出品公司: | EMD Biosciences (Novagen) |
|---|---|
| 别名: | pET41a, pET 41a |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5933 bp |
| 5' 测序引物: | pGEX-5': 5'-GGGCTGGCAAGCCACGTTTGGTG-3' |
| 3' 测序引物: | T7t: 5'-GCTAGTTATTGCTCAGCGG-3' |
| 载体标签: | N-GST, N-His, N-Thrombin, N-EK, N-S |
| 载体抗性: | 卡那 |
| 备注: |
载体带有GST蛋白纯化标签，pET41 a,b,c 的差异是在于多克隆位点。
|
| 产品目录号: | 70556-3 |
| 稳定性: | 瞬时表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3)、C43(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET-41a(+)载体 pET41a质粒基本信息
pET-41a(+)质粒图谱 pET41a载体图谱和pET-41a(+)载体序列 pET41a质粒序列多克隆位点信息
pET-41a(+)质粒 pET41a载体简介
pET41系列载体是设计用来克隆和高水平表达蛋白质的载体，载体融合表达一个含有220个氨基酸的GST标签蛋白纯化片段。pET41a载体的单一的多克隆位点见上面的环状质粒图谱。注意：载体序列是以pBR322质粒的编码规矩进行编码的，所以T7蛋白表达区在质粒图谱上面是反向的。
T7 RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。质粒的F1复制子是被定向的，所以在T7噬菌体聚合酶的作用下，包含有蛋白编码序列的病毒 粒子能够产生，并启动蛋白表达，同时蛋白表达将被T7终止子序列(Cat. No. 69337-3)的作用下终止蛋白翻译。
pET-41a载体上面含有一个EK蛋白酶切位点，当将目的基因使用载体上面的PshAI限制性内切酶位点插入进去时，可以通过EK蛋白酶将载体上的融合的所有氨基酸序列包括GST标签序列，全部切除掉。
pET-41a(+)质粒序列 pET41a载体序列
LOCUS pET-41 a(+) 5933 bp DNA Circular SYN
DEFINITION pET-41 a(+)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..5933
/organism="pET-41 a(+)"
/mol_type="other DNA"
terminator complement(1..120)
/label="T7_terminator"
misc_feature 69..87
/label="T7_Terminal_primer"
CDS complement(139..1095)
/label="ORF frame 3"
misc_feature complement(265..279)
/label="EK"
misc_feature complement(310..354)
/label="S15"
misc_feature complement(397..414)
/label="6xHIS"
gene complement(445..1095)
/label="GST (variant)"
/gene="GST (variant)"
CDS 1096..1824
/label="ORF frame 1"
misc_feature complement(1104..1120)
/label="T7_transl_en_RBS"
misc_feature complement(1138..1165)
/label="lacO"
promoter complement(1165..1183)
/label="T7_promoter"
gene 1219..1482
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
misc_feature complement(1271..1290)
/label="pBRrevBam_primer"
misc_feature 1565..2656
/label="lacI"
CDS 1697..2656
/label="ORF frame 2"
misc_feature 3228..3419
/label="ROP"
misc_feature complement(3413..3435)
/label="pGEX_3_primer"
rep_origin complement(3834..4453)
/label="pBR322_origin"
gene 4559..5374
/label="KanR2"
/gene="KanR2"
CDS 4559..5374
/label="ORF frame 2"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt
181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc
241 ggatccccga tatcccatgg gactcttgtc gtcgtcatca ccggagccac caccggtacc
301 cagatctggg ctgtccatgt gctggcgttc gaatttagca gcagcggttt ctttcatacc
361 aattgcagta ctaccgcgtg gcaccagacc cgcggagtga tggtgatggt gatgaccaga
421 accactagtt gaaccatccg attttggagg atggtcgcca ccaccaaacg tggcttgcca
481 gccctgcaaa ggccatgcta tatacttgct ggatttcaag tacttatcaa tttgtgggat
541 agcttcaata cgttttttaa aacaaactaa ttttgggaac gcatccaggc acattgggtc
601 catgtataaa acaacatcaa gagcgtcata caacatgaag tcaggatggg ttacatgatc
661 accatttaaa tatgttttat gacataaacg atcttcgaac attttcagca tttcaggtag
721 cttgctaaga aaatcaactt tgagagtttc aaagtcttta ctatatgcaa ttctcgaaac
781 accgtatcta atatccaaaa ccgctccttc aagcattgaa atctctgcac gctcttttgg
841 acaaccaccc aacatgttgt gcttgtcagc tatataacgt atgatggcca tagactgtgt
901 taatttaaca tcaccatcaa tataataagg aagattggga aactccaaac ccaattcaaa
961 ctttttgttt cgccatttat caccttcatc gcgctcatac aaatgctctt catatttttc
1021 ttcaagatat tccaaaagaa gtcgagtggg ttgcacaagg cccttaattt tccaataacc
1081 tagtataggg gacatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg
1141 gaattgttat ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga
1201 tcgatctcga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
1261 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
1321 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
1381 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
1441 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaga tcccggacac
1501 catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
1561 cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
1621 ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga
1681 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
1741 ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
1801 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
1861 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
1921 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt
1981 ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat
2041 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
2101 attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct
2161 gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
2221 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
2281 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
2341 cattaccgag tccgggctgc gcgttggtgc ggacatctcg gtagtgggat acgacgatac
2401 cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
2461 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
2521 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac
2581 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
2641 ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta gctcactcat taggcaccgg
2701 gatctcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
2761 gcatgactag catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
2821 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
2881 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
2941 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct
3001 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
3061 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
3121 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
3181 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg
3241 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga
3301 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg
3361 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
3421 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
3481 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
3541 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
3601 agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
3661 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
3721 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
3781 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
3841 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
3901 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
3961 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
4021 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
4081 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
4141 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
4201 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
4261 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
4321 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
4381 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
4441 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
4501 attttggtca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
4561 gagccatatt caacgggaaa cgtcttgctc taggccgcga ttaaattcca acatggatgc
4621 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
4681 tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
4741 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
4801 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
4861 ccccgggaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
4921 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
4981 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
5041 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
5101 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
5161 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
5221 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
5281 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
5341 gcagtttcat ttgatgctcg atgagttttt ctaagaatta attcatgagc ggatacatat
5401 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
5461 cacctgaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
5521 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
5581 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
5641 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
5701 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
5761 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
5821 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
5881 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg cca
//
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pET-41a(+)质粒 pET41a载体应用举例
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