| 质粒名称: | phage UbiC NLS HA stdMCP stdHalo |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 104999 |
| 存储实验室: | Jeffrey Chao |
|
载体骨架基本信息 |
|
| 载体名称: | pHAGE-UbiC |
| 完整质粒大小: | 8881 bp |
| 载体类型: | 哺乳细胞表达\Bacterial Expression, Lentiviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | stdMCP-stdHalo |
| 物种来源: | Synthetic |
| 插入大小: | 2628bp |
| 基因突变: | T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T, N290Y relative to RpBphP2 |
| GenBank编号 | JN247409 |
| 启动子: | UbiC |
| 标签/融合蛋白: |
NLS (N terminal on insert)
HA tag (N terminal on insert)
Two synonymous copies of the MS2 coat protein (N terminal on insert)
Two synonymous copies of the Halo-tag
|
| 克隆方法: | 未知 |
| 5’测序引物 | GTGTGGCACAGCTAGTTCC |
|
文献引用方法 |
|
| 材料和方法部分: | phage UbiC NLS HA stdMCP stdHalo was a gift from Jeffrey Chao (Addgene plasmid # 104999 ; http://n2t.net/addgene:104999 ; RRID:Addgene_104999) |
| 参考文献部分: | Single-Molecule Quantification of Translation-Dependent Association of mRNAs with the Endoplasmic Reticulum. Voigt F, Zhang H, Cui XA, Triebold D, Liu AX, Eglinger J, Lee ES, Chao JA, Palazzo AF. Cell Rep. 2017 Dec 26;21(13):3740-3753. doi: 10.1016/j.celrep.2017.12.008. 10.1016/j.celrep.2017.12.008 PubMed 29281824 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS phage UbiC NLS HA stdMCP stdHalo 8881 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral vector expressing synonymous tandem MS2 coat protein
fused to synonymous tandem Halo-tag for mRNA labeling.
ACCESSION .
VERSION .
KEYWORDS phage UbiC NLS HA stdMCP stdHalo
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8881)
AUTHORS Voigt F, Zhang H, Cui XA, Triebold D, Liu AX, Eglinger J, Lee ES,
Chao JA, Palazzo AF
TITLE Single-Molecule Quantification of Translation-Dependent Association
of mRNAs with the Endoplasmic Reticulum.
JOURNAL Cell Rep. 2017 Dec 26;21(13):3740-3753. doi:
10.1016/j.celrep.2017.12.008.
PUBMED 29281824
REFERENCE 2 (bases 1 to 8881)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8881
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..399
/label=UbC promoter
/note="human ubiquitin C promoter"
regulatory 407..416
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 419..439
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 443..469
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 470..493
/codon_start=1
/product="recognition and cleavage site for human
rhinovirus 3C and PreScission proteases"
/label=HRV 3C site
/translation="LEVLFQGP"
regulatory 1226..1235
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1232..2119
/codon_start=1
/product="modified bacterial dehalogenase that forms
covalent bonds with chloroalkane derivatives"
/label=HaloTag(R)
/translation="MEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
GSEIARWLSTLEISG"
CDS 2153..3037
/codon_start=1
/product="modified bacterial dehalogenase that forms
covalent bonds with chloroalkane derivatives"
/label=HaloTag(R)
/translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
SEIARWLSTLEISG"
misc_feature 3054..3642
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3107..3127)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3525..3536)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 3717..3950
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(4069..4087)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4155..4259
/gene="bla"
/label=AmpR promoter
CDS 4260..5120
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4478..4497)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5291..5879
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5780..5799
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6033..6050
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 6142..6161
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 6290..6311
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6326..6356
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6364..6380
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6369..6391
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 6388..6404
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6388..6404
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 6454..6473
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 6665..7298
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 7345..7470
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 7967..8200
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 8385..8429
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 8722..8839
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
ORIGIN
1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggc ggccgcgcca ccatgggccc
421 aaaaaagaaa agaaaagttg gctaccccta cgacgtgccc gactacgccc tggaagttct
481 gttccagggg cccctgttat taattaacgc ttctaacttt actcagttcg ttctcgtcga
541 caatggcgga actggcgacg tgactgtcgc cccaagcaac ttcgctaacg ggatcgctga
601 atggatcagc tctaactcgc gttcacaggc ttacaaagta acctgtagcg ttcgtcagag
661 ctctgcgcag aatcgcaaat acaccatcaa agtcgaggtg cctaaaggcg cctggcgttc
721 gtacttaaat atggaactaa ccattccaat tttcgccacg aattccgact gcgagcttat
781 tgttaaggca atgcaaggtc tcctaaaaga tggaaacccg attccctcag caatcgcagc
841 aaactccggc atctacgcca tggccagcaa cttcacccag ttcgtgctgg tggacaacgg
901 cggcaccggc gacgtgaccg tggcccccag caacttcgcc aacggcatcg ccgagtggat
961 cagcagcaac agcagaagcc aggcctacaa ggtgacctgc agcgtgagac agagcagcgc
1021 ccagaacaga aagtacacca tcaaggtgga ggtgcccaag ggcgcctgga gaagctacct
1081 gaacatggag ctgaccatcc ccatcttcgc caccaacagc gactgcgagc tgatcgtgaa
1141 ggccatgcag ggcctgctga aggacggcaa ccccatcccc agcgccatcg ccgccaacag
1201 cggcatctac gccgacccac cggtcgccac catggaaatc ggtactggct ttccattcga
1261 cccccattat gtggaagtcc tgggcgagcg catgcactac gtcgatgttg gtccgcgcga
1321 tggcacccct gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt ggcgcaacat
1381 catcccgcat gttgcaccga cccatcgctg cattgctcca gacctgatcg gtatgggcaa
1441 atccgacaaa ccagacctgg gttatttctt cgacgaccac gtccgcttca tggatgcctt
1501 catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt cacgactggg gctccgctct
1561 gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat ttatggagtt
1621 catccgccct atcccgacct gggacgaatg gccagaattt gcccgcgaga ccttccaggc
1681 cttccgcacc accgacgtcg gccgcaagct gatcatcgat cagaacgttt ttatcgaggg
1741 tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc attaccgcga
1801 gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg agctgccaat
1861 cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact ggctgcacca
1921 gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc caccggccga
1981 agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg gcccgggtct
2041 gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc gctggctgtc
2101 tactctggag atttccggtg ggagtggcgg ttctggcggt agcggagggg gtgagatcgg
2161 caccggcttc ccctttgacc ctcactacgt ggaggtgctg ggcgagagga tgcactatgt
2221 ggacgtggga ccaagagatg gaacccccgt gctgttcctg cacggcaacc ccacaagctc
2281 ctacgtgtgg cggaatatca tcccacacgt ggcccccaca cacagatgca tcgccccaga
2341 tctgatcggc atgggcaagt ccgacaagcc cgatctgggc tatttctttg acgatcacgt
2401 gcggttcatg gacgccttta tcgaggccct gggcctggag gaggtggtgc tggtcatcca
2461 cgattggggc tctgccctgg gctttcactg ggccaagcgg aaccccgaga gagtgaaggg
2521 catcgccttc atggagttta tcagaccaat cccaacctgg gatgagtggc ctgagttcgc
2581 cagagagaca ttccaggcct ttcggaccac agacgtgggc agaaagctga tcatcgatca
2641 gaacgtgttt atcgaaggca ccctgcctat gggagtggtg aggccactga cagaggtgga
2701 gatggaccac taccgcgagc ctttcctgaa tccagtggat agggagcctc tgtggcgctt
2761 tcccaacgag ctgcctatcg ccggcgagcc agccaatatc gtggccctgg tggaggagta
2821 tatggactgg ctgcaccagt cccctgtgcc aaagctgctg ttctggggca ccccaggcgt
2881 gctgatccct cccgcagagg cagcaaggct ggccaagtct ctgcccaatt gtaaggccgt
2941 ggatatcggc cctggcctga acctgctgca ggaggacaat cctgatctga tcggcagcga
3001 gatcgcccgc tggctgtcta cactggagat cagcggctaa atcgatagat cctaatcaac
3061 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
3121 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
3181 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
3241 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
3301 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
3361 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
3421 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
3481 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
3541 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
3601 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc
3661 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
3721 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
3781 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
3841 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
3901 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
3961 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
4021 gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac
4081 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
4141 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
4201 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
4261 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
4321 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
4381 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
4441 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
4501 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
4561 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
4621 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
4681 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
4741 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
4801 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
4861 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
4921 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
4981 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
5041 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
5101 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
5161 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
5221 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
5281 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
5341 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
5401 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
5461 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
5521 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
5581 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
5641 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
5701 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
5761 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
5821 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
5881 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
5941 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
6001 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
6061 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
6121 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
6181 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
6241 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
6301 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
6361 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
6421 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
6481 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
6541 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
6601 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
6661 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
6721 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
6781 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
6841 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
6901 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
6961 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
7021 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
7081 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
7141 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
7201 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
7261 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
7321 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
7381 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
7441 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
7501 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
7561 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
7621 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
7681 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
7741 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
7801 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
7861 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
7921 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
7981 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
8041 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
8101 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
8161 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
8221 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
8281 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
8341 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
8401 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
8461 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
8521 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
8581 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
8641 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
8701 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
8761 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
8821 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
8881 t
//
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