pMCP-RecT

LOCUS       pMCP-RecT               10549 bp ds-DNA     circular SYN 05-MAY-2022
DEFINITION  pLenti-EF1A-MCP-EXTEN-RecT-NLS.
ACCESSION   .
VERSION     .
KEYWORDS    pMCP-RecT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Wang C, Qu Y, Cheng JKW, Hughes NW, Zhang Q, Wang M, Cong L
  TITLE     dCas9-based gene editing for cleavage-free genomic knock-in of long 
            sequences.
  JOURNAL   Nat Cell Biol. 2022 Feb;24(2):268-278. doi: 
            10.1038/s41556-021-00836-1. Epub 2022 Feb 10.
  PUBMED    35145221
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 5, 2022 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10549
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2682..3860
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2913..3851
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3808..3828
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3867..3876
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3876..4262
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             5193..5213
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5241..5829
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5294..5314)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5712..5723)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5832..5848)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5833..5849)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6354..6534
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6560..6577)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6566..6790
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6836..7264
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6923..6942)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7133..7154
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7273..7293)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7278..7607
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7458..7593
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7520..7539
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7655..7702
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7721..8095
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8225..8346
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8262..8281)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8316..8335
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8395..8411)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8395..8411)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8408..8430)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8419..8435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8443..8473)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8488..8509
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8626..8643)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8797..9385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8877..8896)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9556..10416)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10179..10198
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10417..10521)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt
     2641 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     2701 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2761 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2821 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2881 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2941 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     3001 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     3061 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     3121 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     3181 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     3241 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     3301 gtgcgcccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     3361 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     3421 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3481 ggccgcttcc cggccctgct gcagggagct caaaatgaag gacgcggcgc tcgggagagc
     3541 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3601 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3661 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3721 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3781 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3841 ttccatttca ggtgtcgtga cgtacggcca ccatggcttc aaactttact cagttcgtgc
     3901 tcgtggacaa tggtgggaca ggggatgtga cagtggctcc ttctaatttc gctaatgggg
     3961 tggcagagtg gatcagctcc aactcacgga gccaggccta caaggtgaca tgcagcgtca
     4021 ggcagtctag tgcccagaag agaaagtata ccatcaaggt ggaggtcccc aaagtggcta
     4081 cccagacagt gggcggagtc gaactgcctg tcgccgcttg gaggtcctac ctgaacatgg
     4141 agctcactat cccaattttc gctaccaatt ctgactgtga actcatcgtg aaggcaatgc
     4201 aggggctcct caaagacggt aatcctatcc cttccgccat cgccgctaac tcaggtatct
     4261 acagcgcttc cggaggatct agcggaggct cctctggctc tgagacacct ggcacaagcg
     4321 agagcgcaac acctgaaagc agcgggggca gcagcggggg gtcaggagga tccatgacaa
     4381 aacaacctcc aatagcaaaa gctgatctgc agaaaaccca gggcaataga gcccctgctg
     4441 ctgtgaagaa tagtgacgtg attagcttca tcaaccagcc tagcatgaag gagcagctgg
     4501 ccgccgccct gcctcgccac atgaccgcag agcggatgat tcggatcgct acaaccgaga
     4561 tcagaaaagt gcccgctctg gggaactgtg ataccatgtc tttcgtgagc gccattgtgc
     4621 agtgtagcca gctgggcctg gagcccggaa gtgccctggg ccacgcctac ctgctgccct
     4681 tcggcaacaa gaacgagaaa tccggtaaaa agaacgtgca gctgatcatc ggttatagag
     4741 gtatgatcga tctggctcgg cggtccggcc aaatcgccag cctgtctgcc agggtggtga
     4801 gagagggcga cgagttcagc tttgagtttg gcctggacga aaagttgatc catcggcccg
     4861 gagagaacga ggacgcccct gtcacccacg tgtatgccgt ggctagactg aaggacggcg
     4921 gtacacagtt cgaagttatg acccggaagc agatcgaact ggtgaggtcc ctgtccaagg
     4981 ccggcaataa cgggccctgg gtgacccact gggaagaaat ggccaagaaa accgccatta
     5041 gacgcctgtt caagtacctg cccgtgtcca ttgagattca gagggccgtg tctatggatg
     5101 agaaagagcc cctgaccatc gaccctgctg acagcagcgt gctgaccggc gagtactctg
     5161 tgattgacaa ttctgaggag tctggtggtt ctcctaagaa aaagaggaag gtgtgagaat
     5221 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     5281 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5341 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5401 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5461 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5521 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5581 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5641 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5701 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5761 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5821 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     5881 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     5941 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     6001 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6061 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     6121 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     6181 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     6241 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     6301 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     6361 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6421 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6481 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     6541 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6601 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     6661 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6721 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     6781 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     6841 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6901 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6961 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7021 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7081 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7141 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7201 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7261 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     7321 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     7381 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     7441 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7501 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     7561 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     7621 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     7681 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     7741 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     7801 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     7861 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     7921 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     7981 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     8041 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     8101 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8161 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8221 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8281 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8341 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     8401 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8461 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8521 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8581 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8641 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8701 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8761 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8821 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8881 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8941 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9001 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9061 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9121 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9181 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9241 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9301 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9361 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9421 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9481 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9541 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9601 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9661 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9721 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9781 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9841 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9901 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9961 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10021 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10081 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10141 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10201 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10261 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10321 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10381 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10441 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10501 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//