| 质粒名称: | mCherry-TIS11B |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 121140 |
| 存储实验室: | Christine Mayr |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 完整质粒大小: | 6853bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | ZFP36L1 (TIS11B |
| 物种来源: | H. sapiens (human) |
|
文献引用方法 |
|
| 材料和方法部分: | mCherry-TIS11B was a gift from Christine Mayr (Addgene plasmid # 121140 ; http://n2t.net/addgene:121140 ; RRID:Addgene_121140) |
| 参考文献部分: | A Membraneless Organelle Associated with the Endoplasmic Reticulum Enables 3'UTR-Mediated Protein-Protein Interactions. Ma W, Mayr C. Cell. 2018 Oct 30. pii: S0092-8674(18)31315-1. doi: 10.1016/j.cell.2018.10.007. 10.1016/j.cell.2018.10.007 PubMed 30449617 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS mCherry-TIS11B 6853 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Mammalian expression of mCherry tagged TIS11B.
ACCESSION .
VERSION .
KEYWORDS mCherry-TIS11B
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6853)
AUTHORS Ma W, Mayr C
TITLE A Membraneless Organelle Associated with the Endoplasmic Reticulum
Enables 3'UTR-Mediated Protein-Protein Interactions.
JOURNAL Cell. 2018 Oct 30. pii: S0092-8674(18)31315-1. doi:
10.1016/j.cell.2018.10.007.
PUBMED 30449617
REFERENCE 2 (bases 1 to 6853)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6853
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 901..1608
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(1046..1064)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 1314..1333
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind complement(2693..2710)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2699..2923
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2969..3397
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3056..3075)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3266..3287
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3406..3426)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3411..3740
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3591..3726
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3653..3672
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3753..4352
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(3753..3772)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4249..4269
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
polyA_signal 4526..4647
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4563..4582)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4617..4636
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4696..4712)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4696..4712)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4709..4731)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4720..4736
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4744..4774)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4789..4810
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4927..4944)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5098..5686)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5178..5197)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5857..6717)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6480..6499
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6718..6822)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
961 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
1021 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
1081 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
1141 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
1201 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
1261 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
1321 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
1381 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
1441 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
1501 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
1561 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagac caccaccctc
1621 gtgtctgcca ccatcttcga cttgagcgaa gttttatgca agggtaacaa gatgctcaac
1681 tatagtgctc ccagtgcagg gggttgcctg ctggacagaa aggcagtggg cacccctgct
1741 ggtgggggct tccctcggag gcactcagtc accctgccca gctccaagtt ccaccagaac
1801 cagctcctca gcagcctcaa gggtgagcca gcccccgctc tgagctcgcg agacagccgc
1861 ttccgagacc gctccttctc ggaagggggc gagcggctgc tgcccaccca gaagcagccc
1921 gggggcggcc aggtcaactc cagccgctac aagacggagc tgtgccgccc ctttgaggaa
1981 aacggtgcct gtaagtacgg ggacaagtgc cagttcgcac acggcatcca cgagctccgc
2041 agcctgaccc gccaccccaa gtacaagacg gagctgtgcc gcaccttcca caccatcggc
2101 ttttgcccct acgggccccg ctgccacttc atccacaacg ctgaagagcg ccgtgccctg
2161 gccggggccc gggacctctc cgctgaccgt ccccgcctcc agcatagctt tagctttgct
2221 gggtttccca gtgccgctgc caccgccgct gccaccgggc tgctggacag ccccacgtcc
2281 atcaccccac cccctattct gagcgccgat gacctcctgg gctcacctac cctgcccgat
2341 ggcaccaata acccttttgc cttctccagc caggagctgg caagcctctt tgcccctagc
2401 atggggctgc ccgggggtgg ctccccgacc accttcctct tccggcccat gtccgagtcc
2461 cctcacatgt ttgactctcc ccccagccct caggattctc tctcggacca ggagggctac
2521 ctgagcagct ccagcagcag ccacagtggc tcagactccc cgaccttgga caactcaaga
2581 cgcctgccca tcttcagcag actttccatc tcagatgact aagaattctg cagatatcca
2641 gcacagtggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
2701 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
2761 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
2821 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
2881 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
2941 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
3001 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
3061 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
3121 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
3181 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
3241 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
3301 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
3361 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
3421 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
3481 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
3541 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
3601 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
3661 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
3721 gaggcctagg cttttgcaaa aagctcccaa ccatgaccga gtacaagccc acggtgcgcc
3781 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
3841 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
3901 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
3961 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
4021 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
4081 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
4141 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
4201 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
4261 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
4321 cctggtgcat gacccgcaag cccggtgcct gagcgggact ctggggttcg aaatgaccga
4381 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
4441 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
4501 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
4561 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
4621 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
4681 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
4741 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
4801 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
4861 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
4921 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
4981 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
5041 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
5101 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
5161 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
5221 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
5281 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
5341 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
5401 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
5461 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
5521 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
5581 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
5641 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
5701 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
5761 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
5821 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
5881 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
5941 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
6001 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
6061 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
6121 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
6181 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
6241 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
6301 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
6361 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
6421 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
6481 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
6541 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
6601 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
6661 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
6721 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
6781 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
6841 gccacctgac gtc
//
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