| 质粒名称: | AAV-P(Per2)-DIO-intron2-dLUC |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 110059 |
| 存储实验室: | Eric Erquan Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | aav2 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4171 bp |
| 完整质粒大小: | 7928 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，AAV，Cre/Lox |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Per2 promoter and luciferase,Per2 intron2 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | Per2 |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | AAV-P(Per2)-DIO-intron2-dLUC was a gift from Eric Erquan Zhang (Addgene plasmid # 110059 ; http://n2t.net/addgene:110059 ; RRID:Addgene_110059) |
| 参考文献部分: | Long-term in vivo recording of circadian rhythms in brains of freely moving mice. Mei L, Fan Y, Lv X, Welsh DK, Zhan C, Zhang EE. Proc Natl Acad Sci U S A. 2018 Apr 2. pii: 1717735115. doi: 10.1073/pnas.1717735115. 10.1073/pnas.1717735115 PubMed 29610316 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
-DIO-intron2-dLUC质粒图谱.png)
载体简介
载体序列
LOCUS AAV-P(Per2)-DIO-intron2-dLUC 7917 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Per2 transcription luciferase reporter.
ACCESSION .
VERSION .
KEYWORDS AAV-P(Per2)-DIO-intron2-dLUC
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7917)
AUTHORS Mei L, Fan Y, Lv X, Welsh DK, Zhan C, Zhang EE
TITLE Long-term in vivo recording of circadian rhythms in brains of freely
moving mice.
JOURNAL Proc Natl Acad Sci U S A. 2018 Apr 2. pii: 1717735115. doi:
10.1073/pnas.1717735115.
PUBMED 29610316
REFERENCE 2 (bases 1 to 7917)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7917
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
protein_bind 689..722
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites (Lee and
Saito, 1988)."
protein_bind 773..806
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS complement(812..931)
/codon_start=1
/product="PEST degradation sequence from mouse ornithine
decarboxylase"
/label=hPEST
/note="human codon-optimized"
/translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
CDS complement(935..982)
/codon_start=1
/product="non-ORF yeast peptide conferring
ubiquitin-dependent degradation"
/label=hCL1
/note="human codon-optimized"
/translation="ACKNWFSSLSHFVIHL"
CDS complement(989..2635)
/codon_start=1
/product="firefly luciferase"
/label=luciferase
/note="synthetic luc2 version of the luciferase gene"
/translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
CDS complement(2645..2665)
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQG"
CDS complement(2690..2734)
/codon_start=1
/product="peptide tag that allows for enzymatic
biotinylation"
/label=AviTag(TM)
/translation="GLNDIFEAQKIEWHE"
CDS complement(2756..2821)
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS complement(3548..3571)
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
regulatory 3571..3580
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
promoter complement(3618..3636)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(3619..3636)
/label=SP6
/note="SP6 promoter, forward primer"
promoter complement(3642..3660)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3703..3722)
/label=pMT2-F
/note="Synthetic intron, forward primer"
intron 3705..3837
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
protein_bind complement(3902..3935)
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites (Lee and
Saito, 1988)."
protein_bind complement(3986..4019)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 4044..4632
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4097..4117)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4515..4526)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 4664..5140
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(4863..4882)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
repeat_region 5180..5320
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 5180..5309
/label=AAV2 ITR
rep_origin 5395..5850
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5482..5501)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5692..5713
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5867..5886)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5986..6008
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6046..6064)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6132..6236
/gene="bla"
/label=AmpR promoter
CDS 6237..7097
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6455..6474)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7268..7856
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7757..7776
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcggatt accgaggctg gtcacgtcgt cgcaggtgat
181 aggccggggg ccctggtctc tgccggctgt gagttgcgca gcggccaagc accattcccc
241 cgcgccgcag tggtacgcgc cactccgggg ctgcacgagc gggccaccgc cgtgccaggt
301 gaatggaagt cccgcaggcc ggaagtggac gagcctactc gcccgggcgc gggggggcgc
361 aagagcgcgc agcatcttca ttgaggaacc cgggcggcga acatggagtt ccatgtgcgt
421 cttatgtaaa gagagcgacg ggcgtctcca ccaattgacg agcgtagctc tcaggttccg
481 ccccgccagt atgcaaatga ggtggcactc cgaccaatgg cgcgcgcagg ggcgggctca
541 gcgcgcgcgg tcacgttttc cactatgtga cagcggaggg cgacgcggcg gcagcggcgc
601 tactgggact agcggctccg ggcggctgcg gcgcaggccg agcgcaccaa gtgacgggcc
661 gagcaaggga tctagagtcg actccggaat aacttcgtat aggatacttt atacgaagtt
721 atgcagaatg gtagctggat tgtagctgct attagcaata tgaaacctct taataacttc
781 gtatagcata cattatacga agttatggtt agacgttgat cctggcgctg gcgcaagcag
841 cagggtgtct atccatgccg ctctcctggg cgcagctcat gggcagggtg ccggcggcct
901 gctcctccac ctcgggaggg aagccgtggc tgttaaggtg gatcacaaag tggcttaagc
961 tactgaacca gttcttgcaa gcagaattca cggcgatctt gccgcccttc ttggccttaa
1021 tgagaatctc gcggatcttg cgggcgtcca acttgccggt cagtccttta ggcacctcgt
1081 ccacgaacac aacaccaccg cgcagcttct tggcggttgt aacctggctg gccacatagt
1141 ccacgatctc cttctcggtc atggttttac cgtgttccag cacgacgact gcggcgggca
1201 gctcgccggc atcgtcgtcg ggcaggccgg cgaccccggc gtcgaagatg ttggggtgtt
1261 gcagcaggat gctctccagt tcggctgggg ctacctggta gcccttgtat ttgatcaggc
1321 tcttcagccg gtccacgatg aagaagtgct cgtcctcgtc ccagtaggcg atgtcgccgc
1381 tgtgcagcca gccgtccttg tcgatgagag cgtttgtagc ctcggggttg ttaacgtagc
1441 cgctcatgat catggggcca cggacgcaca gctcgccgcg ctggttcaca cccagtgtct
1501 taccggtgtc caagtccacc accttagcct cgaagaaggg caccaccttg cctactgcgc
1561 caggcttgtc gtccccttcg ggggtgatca gaatggcgct ggttgtttct gtcaggccgt
1621 agccctggcg gatgcctggt aggtggaagc gtttggccac ggcctcacct acctccttgc
1681 tgagcggcgc cccgccgctg gcgatctcgt gcaagttgct taggtcgtac ttgtcgatga
1741 gagtgctctt agcgaagaag ctaaatagtg tgggcaccag cagggcagat tgaatcttat
1801 agtcttgcaa gctgcgcaag aatagctcct cctcgaagcg gtacatgagc acgacccgaa
1861 agccgcagat caagtagccc agcgtggtga acatgccgaa gccgtggtga aatggcacca
1921 cgctgaggat agcggtgtcg gggatgatct ggttgccgaa gatggggtcg cgggcatgac
1981 tgaatcggac acaagcggtg cggtgcggta gggctacgcc cttgggcaat ccggtactgc
2041 cactactgtt catgatcagg gcgatggttt tgtcccggtc gaagctctcg ggcacgaagt
2101 cgtactcgtt gaagccgggt ggcaaatggg aagtcacgaa ggtgtacatg ctttggaagc
2161 cctggtagtc ggtcttgcta tccatgatga tgatcttttg tatgatcggt agcttctttt
2221 gcacgttgag gatcttttgc agccctttct tgctcacgaa tacgacggtg ggctggctga
2281 tgcccatgct gttcagcagc tcgcgctcgt tgtagatgtc gttagctggg gccacagcca
2341 caccgatgaa cagggcaccc aacacgggca tgaagaactg caagctattc tcgctgcaca
2401 ccacgatccg atggtttgta ttcagcccat agcgcttcat agcttctgcc agccgaacgc
2461 tcatctcgaa gtactcggcg taggtaatgt ccacctcgat atgtgcgtcg gtaaaggcga
2521 tggtgccggg caccagggcg tagcgcttca tggctttgtg cagctgctcg ccggcggtcc
2581 cgtcttcgag tgggtagaat ggcgctgggc ccttcttaat gtttttggca tcttcacctg
2641 cagggccctg aaaatacagg ttttcggatc ctgaaccgcc tgccgatccc tcatgccact
2701 ctattttctg agcttcgaaa atatcgttca ggccgctagc tgcggccgct ccacccttat
2761 cgtcatcgtc cttgtagtcg atgtcatgat ctttataatc accgtcatgg tctttgtagt
2821 cctgcaaagg aagatagata gataagatct ccacacgcct cttcccccaa ccagccgata
2881 aaactgacca gggacatcac accagagatt ttaaagaaaa tattaccgta tgctgggtgg
2941 tggctttggg acaaaggact tgcctggagt gcctgagacc catagtatgg cctccatact
3001 tcccctcaaa ataaaagtca gagctggaga atcagctcaa tggtgaagag aagcacccac
3061 acggctgctt acacccatct gtaactcaca gttccagggg atccaatgcc ctcttctgtc
3121 ccctatggtc accaaacatg caagtggtgc accgacatac atacaggcaa aacacccata
3181 tactttaaag taaaaattta aaaaaggaaa agtcatgtta aaactgaaac ataagagctt
3241 cagctattag gagacttgaa cactgagaga cagaaatgaa aaggaatatt ctgtgatcga
3301 atgcatgtgt gtgagcaggc tcctccgagg tcttactgga ctgtctgtta agtgcatcag
3361 atacaaaaat gcaaagatga cgtggtatcg ttatttggaa ggacaaagta caataaatac
3421 catctacagg attaagaagt actgccatcc tgcaagtcct ggtacttgct gcttgaagac
3481 gggcgacaaa ctatggaggg agccacccgt gtcctgtgtg cgagggcctg gcctgaggct
3541 gacctacctt gtcatcgtca tccttgtagt ccatggtggc ctctccttcg agtgcaaaaa
3601 gaacaagtag cttgtattct atagtgtcac ctaaatgcta gcctatagtg agtcgtatta
3661 agtactctag ccttaagagc tgtaattgaa ctgggagtgg acacctgtgg agagaaaggc
3721 aaagtggatg tcagtaagac caataggtgc ctatcagaaa cgcaagagtc ttctctgtct
3781 cgacaagccc agtttctatt ggtctcctta aacctgtctt gtaaccttga tacttacctg
3841 cccagtgcct cacgaccaac tttcgctacc ttaggaccgt tatagttact gcagcgctag
3901 cataacttcg tataaagtat cctatacgaa gttatttgcc ttaacccaga aattatcact
3961 gttattcttt agaatggtgc aaagaataac ttcgtataat gtatgctata cgaagttatg
4021 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
4081 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
4141 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
4201 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
4261 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
4321 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
4381 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
4441 catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct
4501 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
4561 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
4621 ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga
4681 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt
4741 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg
4801 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg
4861 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc
4921 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg
4981 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc
5041 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct
5101 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac
5161 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
5221 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
5281 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
5341 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
5401 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
5461 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
5521 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
5581 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
5641 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
5701 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
5761 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
5821 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
5881 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
5941 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
6001 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
6061 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
6121 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
6181 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
6241 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
6301 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
6361 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
6421 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
6481 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
6541 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
6601 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
6661 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
6721 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
6781 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
6841 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
6901 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
6961 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
7021 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
7081 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
7141 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
7201 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
7261 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
7321 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
7381 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
7441 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
7501 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
7561 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
7621 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
7681 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
7741 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
7801 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
7861 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
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