| 质粒名称: | pGM1192 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 115683 |
| 存储实验室: | Günther Muth |
|
载体骨架基本信息 |
|
| 载体名称: | pSG5 |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 7393 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 载体抗性: | 阿普霉素，25μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | mCherry, aac(3)IV, tsr |
| 物种来源: | 合成的链霉菌属 |
| 插入大小: | -- |
| 基因突变: | 链霉菌的mCherry密码子优化 |
| GenBank编号 | -- |
| 启动子: | SF14* |
| 标签/融合蛋白: | mCherry上的Strep-tagII，密码子优化（插入物上的N末端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | M13 Reverse |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGM1192 was a gift from Günther Muth (Addgene plasmid # 115683 ; http://n2t.net/addgene:115683 ; RRID:Addgene_115683) |
| 参考文献部分: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGM1192 7393 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION E. coli-Streptomyces shuttle vector for gene expression.
ACCESSION .
VERSION .
KEYWORDS pGM1192
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7393)
TITLE E.coli-Streptomyces shuttle vectors
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7393)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7393
/organism="synthetic DNA construct"
/mol_type="other DNA"
oriT 466..575
/note="incP origin of transfer"
CDS 891..1691
/codon_start=1
/gene="aac(3)IV"
/product="aminoglycoside 3-N-acetyltransferase type IV
"
/label=ApmR
/note="confers resistance to apramycin (Paget and Davies,
1996)"
/translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDSFWRLPNV
KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
LAELLAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
rep_origin 1993..2581
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2482..2501
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2735..2752
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2869..2890
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2905..2935
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2943..2959
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2948..2970
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2967..2983
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2967..2983
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 3075..3098
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
primer_bind 3709..3732
/label=DsRed1-C
/note="DsRed1, forward primer"
terminator 3992..4040
/label=fd terminator
/note="central terminator from bacteriophage fd (Otsuka and
Kunisawa, 1982)"
CDS 5755..7197
/codon_start=1
/gene="rep"
/product="replication initiator protein from the
Streptomyces ghanaensis plasmid pSG5"
/label=pSG5 Rep
/translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
APVLQPWGQHPGDCLRVDTAVA"
ORIGIN
1 ggccgtcgac gacgacgcgt ccgcctgcct gcttttggcc ggcgggccgg gccgcggttg
61 tccgccgggt cggaccggtc ccttggtcga ccgatgcctc ggccacgacg atgctgggcc
121 cgaagcggta cgcgacccgt gccgggccgc agcggatcgc ggcagcagga gctccccgcc
181 cgccacggct ggctggatgg tcgcctcacg cgcccgcagg ggtgacggcg tgggtgcggc
241 ggtcagcccc gtgatcgggc gtcagacggc cgctgaggga ccgcgaccac ccctcccttc
301 ccgcctgccg tctttcaccg cccggccggt ccgcagacgc cgtacggcca ggccacggag
361 gattacgggc agatggcgcc cacccggcac ccgggctggg gcaccctgga cagcacgaag
421 gtccgcccca ctcaccgggg cgggccttcg cgttgagcta gagttcccgc cagcctcgca
481 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
541 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
601 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata
661 ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaat gcagctcacg
721 gtaactgatg ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa
781 atgccggcct ttgaatgggt tcatgtgcag ctccataagc aaaaggggat gataagttta
841 tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta tgatcgactg atgtcatcag
901 cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc cgagctcatc ggtcagcttc
961 tcaaccttgg ggttaccccc ggcggtgtgc tgctggtcca cagctccttc cgtagcgtcc
1021 ggcccctcga agatgggcca cttggactga tcgaggccct gcgtgctgcg ctgggtccgg
1081 gagggacgct cgtcatgccc tcgtggtcag gtctggacga cgagccgttc gatcctgcca
1141 cgtcgcccgt tacaccggac cttggagttg tctctgactc attctggcgc ctgccaaatg
1201 taaagcgcag cgcccatcca tttgcctttg cggcagcggg gccacaggca gagcagatca
1261 tctctgatcc attgcccctg ccacctcact cgcctgcaag cccggtcgcc cgtgtccatg
1321 aactcgatgg gcaggtactt ctcctcggcg tgggacacga tgccaacacg acgctgcatc
1381 ttgccgagtt gttggcaaag gttccctatg gggtgccgag acactgcacc attcttcagg
1441 atggcaagtt ggtacgcgtc gattatctcg agaatgacca ctgctgtgag cgctttgcct
1501 tggcggacag gtggctcaag gagaagagcc ttcagaagga aggtccagtc ggtcatgcct
1561 ttgctcggtt gatccgctcc cgcgacattg tggcgacagc cctgggtcaa ctgggccgag
1621 atccgttgat cttcctgcat ccgccagagg cgggatgcga agaatgcgat gccgctcgcc
1681 agtcgattgg ctgagctcat aagttcctat tccgaagttc ctattcgaaa tgaccgacca
1741 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
1801 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
1861 gctggagttc ttcgcccacc ccaaaaggat ctaggtgaag atcctttttg ataatctcat
1921 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
1981 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
2041 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
2101 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
2161 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
2221 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
2281 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
2341 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
2401 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
2461 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
2521 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
2581 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
2641 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
2701 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
2761 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
2821 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
2881 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
2941 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaattgtac
3001 gtagacctac gccttgacct tgatgaggcg cgtgagctac aatcaatact cgattagaag
3061 ggaggtgaca tatgtggagc cacccgcagt tcgagaaggg atccgtctcc aagggcgagg
3121 aggacaacat ggccatcatc aaggagttca tgcgcttcaa ggtccacatg gagggctccg
3181 tcaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccgtac gagggcaccc
3241 agaccgccaa gctgaaggtc accaagggcg gcccgctgcc gttcgcctgg gacatcctgt
3301 ccccgcagtt catgtacggc tccaaggcct acgtcaagca cccggccgac atcccggact
3361 acctgaagct gtccttcccg gagggcttca agtgggagcg cgtcatgaac ttcgaggacg
3421 gcggcgtcgt caccgtcacc caggactcct ccctgcagga cggcgagttc atctacaagg
3481 tcaagctgcg cggcaccaac ttcccgtccg acggcccggt catgcagaag aagaccatgg
3541 gctgggaggc ctcctccgag cgcatgtacc cggaggacgg cgccctgaag ggcgagatca
3601 agcagcgcct gaagctgaag gacggcggcc actacgacgc cgaggtcaag accacctaca
3661 aggccaagaa gccggtccag ctgccgggcg cctacaacgt caacatcaag ctggacatca
3721 cctcccacaa cgaggactac accatcgtcg agcagtacga gcgcgccgag ggccgccact
3781 ccaccggcgg catggacgag ctgtacaagt gaagcttaga tccccgggta ccgagctcga
3841 attccccaga tctaaagttt tgtcgtcttt ccagacgtta gtaaatgaat tttctgtatg
3901 aggttttgct aaacaacttt caacagtttc agcggagtga gaatagaaag gaacaactaa
3961 aggaattgcg aataataatt ttttcacgtt gaaaatctcc aaaaaaaaag gctccaaaag
4021 gagcctttaa ttgtatcggt ttatcagctt gctttcgagg tgaatttctt aaacagcttg
4081 ataccgatag ttgcgccgac aatgacaaca accatcgccc acgcataacc gatatattcg
4141 gtcgctgagg cttgcaggga gtcaaaggcc gcttttgcgg gatctcgtcg aaggcactag
4201 aggcctatat gcggggatcg accgcgcggg tcccggacgg ggaagagcgg ggagctttgc
4261 cagagagcga cgacttcccc ttgcgttggt gattgccggt cagggcagcc atccgccatc
4321 gtcgcgtagg gtgtcacacc ccaggaatcg cgtcactgaa cacagcagcc ggtaggacga
4381 ccatgactga gttggacacc atcgcaaatc cgtccgatcc cgcggtgcag cggatcatcg
4441 atgtcaccaa gccgtcgcga tccaacataa agacaacgtt gatcgaggac gtcgagcccc
4501 tcatgcacag catcgcggcc ggggtggagt tcatcgaggt ctacggcagc gacagcagtc
4561 cttttccatc tgagttgctg gatctgtgcg ggcggcagaa cataccggtc cgcctcatcg
4621 actcctcgat cgtcaaccag ttgttcaagg gggagcggaa ggccaagaca ttcggcatcg
4681 cccgcgtccc tcgcccggcc aggttcggcg atatcgcgag ccggcgtggg gacgtcgtcg
4741 ttctcgacgg ggtgaagatc gtcgggaaca tcggcgcgat agtacgcacg tcgctcgcgc
4801 tcggagcgtc ggggatcatc ctggtcgaca gtgacatcac cagcatcgcg gaccggcgtc
4861 tccaaagggc cagccgaggt tacgtcttct cccttcccgt cgttctctcc ggtcgcgagg
4921 aggccatcgc cttcattcgg gacagcggta tgcagctgat gacgctcaag gcggatggcg
4981 acatttccgt gaaggaactc ggggacaatc cggatcggct ggccttgctg ttcggcagcg
5041 aaaagggtgg gccttccgac ctgttcgagg aggcgtcttc cgcctcggtt tccatcccca
5101 tgatgagcca gaccgagtct ctcaacgttt ccgtttccct cggaatcgcg ctgcacgaga
5161 ggatcgacag gaatctcgcg gccaaccgat aagcgcctct gttcctcgga cgctcggttg
5221 ctcgacctcg attcgtcagt gatgatcaac aggacccaga aatggcacga gcccgggatt
5281 ggcctcccgg gctcgccgcc cccgaaccgg cgcagcatgc cgagtgagga cgtttgctct
5341 gtgacgagcg tacgagagaa cagcgcgtcg acaccaccgc gccccgccgg accgaagtgc
5401 cactgcggca ccccgatcgt ccacatcccc gggaagcgcc ccaaaatcta ctgctcggac
5461 gcctgccgaa agcgcgcaaa gcgtctcatt gcggaccagt cacgcagcgt agcaagcacg
5521 actacagcgg gtaatccgga cgcgggcttt agtagtaagg gaaaatctcc gtccactgca
5581 ggtcagagcg ctgtaactgg aacgggttct accccaaacg agggtgccgc gacagccggg
5641 cgggaggacc cgcgcgacga gcggttcgcc cggcgggacc ggcaccagac ggtgtcgctg
5701 aacgaggcgt tcaagggctg cgggacccgg ctcactgccg ggacggccga gctgatgtgg
5761 aagccgggcg aggcgacgtg gggcaacacg tgccgctgca acaacgtcca cacctgcccg
5821 tggtgcatga gccgcatcct ggccgtacgg ggctcgaacg tgcagctcgc cgccgacggt
5881 ctcgcggacg ccgggtacgg gctgcacctt ggcacgaaca cgttgcgcca cttcgagcgc
5941 atggcgttcg gcaccgtccg caagggaatg cggcacggcc tggtcgcggt cttgcacgac
6001 ggctggaagg gcgcgtacgg ctcgtccggc cgccgctggc gcacgatgcg cgacgacttc
6061 gggatcatcg gctacgagcg ggccttcgag gacaccttcg gctggggctc cggctggcac
6121 ctgcactggc acacgctctg ggtcacccgc gaggtgctcg gcccggacgc ccaggccgcg
6181 ttccgcgacg cactcgccgg agcctgggcc gccggggtcg aatccgcggg cggctacacc
6241 gtcagcgaga cgtgcgaccg gcccggttgc tcctgtgagg gcaagggcca cggcaccgac
6301 gtgcgcccac tcaacggcgc ggacgccgct gacggcgatg cgggcaagca ggcccgctac
6361 ctctacaagg acggcgacaa gaccaagggc ggcgtcgcca agatcggcct ggagctggcc
6421 ggacagaact tcaaggccgg tcgcggcgac gaccgcatgg gaccgctcga cctgggcgac
6481 gcggcggccg ccgagctgca gcggcttcgt cggcccggcc cgttcgtcga gaagtaccgc
6541 gagcgcgagt tcggcgtctt ccaggtccgc aagcactacc ggtcgcagaa cctgaaccgg
6601 ctgatcaagg aactcggcat ccagcaggac gtccgcaccg aggaggagat caccgacgac
6661 accgagggcc tggtcgccat cgcggtcatc ccggcctaca tctggtaccg atacatcgcc
6721 cgcgtcgccg gacgtcgcct cgacctcatc aaggtcgccg agacgtacgg cctaccgggc
6781 gtccggcggc tcgtcgagtc ctggggcctg gtgtggggca aggacgtcct ggacccgccc
6841 gccccggaag cgccggccgc gccgggcgac cttgacgctg accagatgag gttcgaggtc
6901 atgtccgagg aggaagctgc tttccgcgag gcgcgccgga aggccaacga ggcccgcacg
6961 gaggagctgg ccgcttcgct cgaccgagtg cgtcagccga agaaggaggc gatccgcccg
7021 acgatcagcc ttcggaagcg cctgaagccg aagccggtca ccgtcgacgt caagactccc
7081 ccgcccggcg ccgcgtcgcc cgtgtgccgc cgctgcaagg gcaagctcgc cccggtactc
7141 cagccctggg gccagcaccc cggagactgc ctccgcgtcg acacagcggt cgcgtagggc
7201 cgctgaggga ccgccatgag accgccaccc cggacatccg ggtggcggtc tcatggcggt
7261 cccggcgcga aagccgaggg tctgcctgcc gtgaggtggc cggcggacct gggttactgc
7321 tggaatcccg cgcccagtcc gagcgtccgc cggcgctttt cacctggctc cgggtgctcg
7381 tcgatgagca cca
//
